Analysis of Tembusu virus infection of human cell lines and human induced pluripotent stem cell derived hepatocytes

Ruangrung, Kanyarat; Chakritbudsabong, Warunya; Rungarunlert, Sasitorn; Smith, Duncan R.; Hongeng, Suradej; Sirinonthanawech, Naraporn; Boonarkart, Chompunuch; Pulmanausahakul, Rojjanaporn; Suptawiwat, Ornpreya; Auewarakul, Prasert

doi:10.1016/j.virusres.2020.198252

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…A recent investigation on TMUV cell tropism was carried out using various human cell lines. The authors pointed out the susceptibility of liver, kidney and nerve cell lines at various degrees, while the lung, muscle, B cells, T cells and monocytes were largely resistant to the infection [ 96 ]. All these reports indicate that TMUV is able to replicate in a wide range of vertebrate cells.…”

Section: Clinical Features and Pathogenicitymentioning

confidence: 99%

New Insights into the Biology of the Emerging Tembusu Virus

et al. 2021

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Reported for the first time in 1955 in Malaysia, Tembusu virus (TMUV) remained, for a long time, in the shadow of flaviviruses with human health importance such as dengue virus or Japanese encephalitis virus. However, since 2010 and the first large epidemic in duck farms in China, the threat of its emergence on a large scale in Asia or even its spillover into the human population is becoming more and more significant. This review aims to report current knowledge on TMUV from viral particle organization to the development of specific vaccines and therapeutics, with a particular focus on host–virus interactions.

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Section: Clinical Features and Pathogenicitymentioning

confidence: 99%

New Insights into the Biology of the Emerging Tembusu Virus

et al. 2021

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“…Capable of infecting nearly all species of ducks, it inflicts a mortality rate of 5 to 30% and can cause a drastic decrease in egg production, reaching up to 90%, resulting in substantial economic losses within the duck industry ( Lv et al, 2019 ). Intriguingly, TMUV exhibits the ability to infect mammalian cells, including human nerve and liver cell lines ( Ruangrung et al, 2021 ), and TMUV-specific antibodies have been detected in duck farm workers ( Tang et al, 2013 ), implying a potential threat to humans. Regrettably, there is currently no specific therapeutic treatment available for TMUV-induced diseases, highlighting the urgent need for preventive measures.…”

Section: Introductonmentioning

confidence: 99%

NS1: a promising novel target antigen with strong immunogenicity and protective efficacy for avian flavivirus vaccine development

Huang,

Wang,

et al. 2024

Poultry Science

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“…In 2010, TMUV caused the first epidemic in ducks in China and then rapidly spread to several Asian countries, resulting in huge economic losses to the poultry industry [9][10][11]. Moreover, TMUV was found to infect other species, such as chickens, sparrows, and geese [12,13], and replicate efficiently in both human nerve and liver cell lines [14]. A surveillance study showed that 71.9% of the investigated duck farm workers were seropositive to TMUV, and TMUV RNA was detected in 47.7% of oral swabs [11,15].…”

Section: Introductionmentioning

confidence: 99%

326K at E Protein Is Critical for Mammalian Adaption of TMUV

Liu,

Yan,

Peng

et al. 2023

Viruses

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Outbreaks of Tembusu virus (TMUV) infection have caused huge economic losses to the poultry industry in China since 2010. However, the potential threat of TMUV to mammals has not been well studied. In this study, a TMUV HB strain isolated from diseased ducks showed high virulence in BALB/c mice inoculated intranasally compared with the reference duck TMUV strain. Further studies revealed that the olfactory epithelium is one pathway for the TMUV HB strain to invade the central nervous system of mice. Genetic analysis revealed that the TMUV HB virus contains two unique residues in E and NS3 proteins (326K and 519T) compared with duck TMUV reference strains. K326E substitution weakens the neuroinvasiveness and neurovirulence of TMUV HB in mice. Remarkably, the TMUV HB strain induced significantly higher levels of IL-1β, IL-6, IL-8, and interferon (IFN)-α/β than mutant virus with K326E substitution in the brain tissue of the infected mice, which suggested that TMUV HB caused more severe inflammation in the mouse brains. Moreover, application of IFN-β to infected mouse brain exacerbated the disease, indicating that overstimulated IFN response in the brain is harmful to mice upon TMUV infection. Further studies showed that TMUV HB upregulated RIG-I and IRF7 more significantly than mutant virus containing the K326E mutation in mouse brain, which suggested that HB stimulated the IFN response through the RIG-I-IRF7 pathway. Our findings provide insights into the pathogenesis and potential risk of TMUV to mammals.

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Analysis of Tembusu virus infection of human cell lines and human induced pluripotent stem cell derived hepatocytes

Cited by 6 publications

References 21 publications

New Insights into the Biology of the Emerging Tembusu Virus

New Insights into the Biology of the Emerging Tembusu Virus

NS1: a promising novel target antigen with strong immunogenicity and protective efficacy for avian flavivirus vaccine development

326K at E Protein Is Critical for Mammalian Adaption of TMUV

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