Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana

Bala, Shashi; Uniyal, G. C.; Chattopadhyay, Sunil K.; Tripathi, V.; Sashidhara, Koneni V.; Kulshrestha, Manish; Sharma, Ram Prakash; Jain, S. P.; Kukreja, A. K.; Kumar, Sushil

doi:10.1016/s0021-9673(99)00841-9

Cited by 46 publications

(24 citation statements)

References 24 publications

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“…Analysis of extracts from Taxus species is usually made on C 18 (Ballero et al, 2003;Nadeem et al, 2002), C 8 (Lauren et al, 1995), phenyl (Theodoridis et al, 1998;Bala et al, 1999), biphenyl (Kethchum and Gibson, 1993) and cyano phases (Auriola et al, 1992). In most studies, C 18 columns were used to analyze samples.…”

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

“…Many detection wavelengths for taxoids have been reported, such as 227 nm (Lauren et al, 1995), 228 nm (Bala et al, 1999) and 250 nm (Aboul-Enein and Serignese, 1996). Considering the maximal absorption as criterion, we chose 234 nm as the optimal wavelength for the detection of the six taxoids.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

“…Many gradient conditions and alternative options were tested to optimize the HPLC separation, especially for the main taxoids in Taxus extracts, such as paclitaxel, 10-DAB III and cephalomannine (Theodoridis et al, 1998;Mroczek and Glowniak, 2001;Bala et al, 1999). In the present study, the effects of different mobile phase compositions, such as acetonitrile-water, methanol-water and methanol-acetonitrile-water on the separation performances of the six taxoids were studied and the effect of adding acetic acid or tetrahydrofuran to the mobile phase to regulate the pH value or polarity was also investigated.…”

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

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Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.

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Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

Section: Optimization Of Chromatographic Conditionsmentioning

confidence: 99%

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Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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“…Although there is sporadic reports on endophytic fungus growing on Taxus sp. and some other gymnosperms (Bala et al 1999;Saikia et al 2005) in NE India, yet its rich biodiversity needs to be explore for the benefit of the society. Considering the increasing demand of pharmaceutical sectors for novel drug and drug intermediate to control dreaded diseases like HIV, cancer and dominant infectious diseases, it was our endeavor to characterize the antimicrobial property of an endophytic fungus isolated from high altitude gymnosperms Taxus wallichiana, mostly known for its anticancer property.…”

Section: Introductionmentioning

confidence: 99%

Optimization of process parameters for improved production of bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India

Gogoi

Boruah

Saikia

et al. 2007

World J Microbiol Biotechnol

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“…The high demand for paclitaxel for clinical use has led to the intense study of other forms of production, including the sustainable harvesting of Taxus species, and partial synthesis from related compounds. Numerous related compounds have been identified, including those more commonly cited like 10-deacetylbaccatin III (10-DAB III), baccatin III, and cephalomannine [1,8].…”

Section: Introductionmentioning

confidence: 99%

Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

Fu²,

et al. 2006

J of Separation Science

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A method is described for the simultaneous determination of paclitaxel and three related taxoids, 10-deacetylbaccatin III (10-DAB III), baccatin III, and cephalomannine, in the extracts from the needles of three Chinese yew species, Taxus cuspidata, T. chinensis, and T. media. SPE was applied as the sample preparation technique and RP-HPLC with a photodiode array detector (PAD) was used for the analysis of extract samples. The crude extracts were treated with an improved SPE cartridge packed with a combination of 1-vinyl-pyrrolidin-2-one and divinyl-benzene. The eluent was 75% methanol. The following separation was achieved with a gradient program on an HIQ SIL C18W column in a system of ACN/water within 60 min. The samples were detected by PAD at wavelengths of 232.1 nm for 10-DAB III, 229.8 nm for baccatin III and paclitaxel, and 223.9 nm for cephalomannine. The content of 10-DAB III, baccatin III, cephalomannine, and paclitaxel varied from 0.0277 to 0.0875, 0.0254 to 0.0405, 0.0715 to 0.2486, and 0.0996 to 0.1301 mg/g in fresh needles of the above yew species, respectively. The assay achieved good resolution in the separation between the four compounds, and it can be used for quality control or purity determination for those in bulk and pharmceutical dosage forms.

show abstract

Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana

Cited by 46 publications

References 24 publications

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Optimization of process parameters for improved production of bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India

Rapid separation of four main taxoids in Taxus species by a combined LLP‐SPE‐HPLC (PAD) procedure

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