Optimization of process parameters for improved production of bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India

Gogoi, Dhrubajyoti; Boruah, Hari Prasanna Deka; Saikia, Ratul; Bora, Tarun C.

doi:10.1007/s11274-007-9442-3

Cited by 52 publications

(25 citation statements)

References 34 publications

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“…The pH of culture media is one of the very important determining parameters for the metabolism and hence for the biosynthesis of secondary metabolites. Permeability characteristics of the cell wall and membrane are also related to the cell environment pH value and thus have got the effect on either ion uptake or loss to the nutrient medium [33].…”

Section: Media Optimizationmentioning

confidence: 99%

Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root.

Verma¹,

Lal²,

Debnath³

2017

Asian J Pharm Clin Res

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Objective: To study the antimicrobial activity of crude ethyl acetate extract from endophytic fungus in Calotropis procera root.Methods: Endophytic fungus was screened for production of antimicrobial metabolites. Fermentation was carried out in 500 ml Erlenmeyer flask. Disc diffusion method was used to test antimicrobial activity of crude extract using chloramphenicol sulfate and fluconazole as a positive control.Results: A total of 14 endophytic fungi were isolated (CPR1-CPR14). Among these 14 isolates, CPR5 was found to show maximum antimicrobial activity, in compare to other isolates, against Gram-positive, Gram-negative bacteria and fungi. The antimicrobial activity was tested in opposition to Escherichia coli, Pseudomonas aeroginosa, Bacillus subtilis, Staphylococcus aureus, Ralstonia solanacearum, Xanthomonas oryzae, Penicillium chrysogenum, Candida albicans, Phoma exigua, Sclerotium rolfsii, and Sclerotinia scleratiourum. Minimum inhibitory concentration of crude extract against test microorganisms was determined. Fungus was identified as Aspergillus sp. production parameters (temperature, pH, carbon source, nitrogen source, and sodium chloride concentration) were optimized. Conclusion:Crud extract produced by the isolated endophytic fungus could be an important source of broad-spectrum antimicrobial metabolites.

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Section: Media Optimizationmentioning

confidence: 99%

Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root.

Verma¹,

Lal²,

Debnath³

2017

Asian J Pharm Clin Res

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“…On the basis of the results, sucrose and NaNO 3 were found to be significant, thus, endorsing the important role played by carbon and nitrogen source to regulate secondary metabolite production and the microbial growth [26]. …”

Section: Resultsmentioning

confidence: 99%

Optimization of Antioxidant Potential of Penicillium granulatum Bainier by Statistical Approaches

Chandra

Arora

2012

ISRN Microbiology

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A three-step optimization strategy which includes one-factor-at-a-time classical method and different statistical approaches (Plackett-Burman design and response surface methodology) that were applied to optimize the antioxidant potential of Penicillium granulatum. Antioxidant activity was assayed by different procedures and compared with total phenolic content. Primarily, different carbon and nitrogen sources were screened by classical methods, which revealed sucrose and NaNO3 to be the most suitable. In second step, Plackett-Burman design also supported sucrose and NaNO3 to be the most significant. In third step, response surface analysis showed 4.5% sucrose, 0.1% NaNO3, and incubation temperature of 25°C to be the optimal conditions. Under these conditions, the antioxidant potential assayed through different procedures was 78.2%, 70.1%, and 78.9% scavenging effect for DPPH radical, ferrous ion, and nitric oxide ion, respectively. The reducing power showed an absorbance of 1.6 with 68.5% activity for FRAP assay.

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“…The results got further support from the RSM observations where low concentration of NaNO 3 in the medium favours the antioxidant activity. This demonstrates the important role of the nitrogen source to regulate secondary metabolite production [20]. Sucrose is beneficial for the growth of fungi as well as for the production of secondary metabolites that are responsible for their antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Optimization of antioxidant potential of Aspergillus terreus through different statistical approaches

Arora

Chandra

2010

Biotech and App Biochem

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A three-step optimization strategy, which includes a one-factor-at-a-time classical method and different statistical approaches [Plackett-Burman design and RSM (response surface methodology)], were applied to optimize the antioxidant potential of Aspergillus terreus. Antioxidant activity was assayed by different procedures and compared with TPC (total phenolic content). Primarily, different carbon and nitrogen sources were screened by classical methods, which revealed sucrose and NaNO3 to be the most suitable. The significance of the components of Czapek Dox's medium with respect to antioxidant activity was evaluated with the Plackett-Burman design, which supported sucrose and NaNO3 to be the most significant. In a second step, sucrose and NaNO3 along with temperature were further taken as three variables for RSM to study their interaction. Response surface analysis showed 4% sucrose, 0.1% NaNO3 and an incubation temperature of 30 °C to be the optimal conditions. Under these conditions, the antioxidant potential assayed through the different procedures was 88.1, 74.9 and 70.2% scavenging effect for DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, ferrous ion and nitric oxide ion respectively. The reducing power showed an absorbance of 2.0 with 71.5% activity for the FRAP (ferric reducing antioxidant power) assay. TPC under different physio-chemical conditions and antioxidant potential under various assay procedures correlated positively.

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Optimization of process parameters for improved production of bioactive metabolite by a novel endophytic fungus Fusarium sp. DF2 isolated from Taxus wallichiana of North East India

Cited by 52 publications

References 34 publications

Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root.

Optimization of process parameters for production of antimicrobial metabolites by an endophytic fungus Aspergillus sp. CPR5 isolated from Calotropis procera root.

Optimization of Antioxidant Potential of Penicillium granulatum Bainier by Statistical Approaches

Optimization of antioxidant potential of Aspergillus terreus through different statistical approaches

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