Analysis of stylar self-incompatibility competence by use of heat induced inactivation

Abstract

Immersion of Lilium longiflorum pistils in 49 °C water for increasing durations of 1,2,3, or 4 minutes immediately prior to incompatible pollination resulted in a correspondingly progressive decrease in the stylar self-incompatibility competence, as determined from the lengths attained by pollen tubes during 48 hours growth in the styles at 24 °C. Neither pistils remaining on the plant nor those detached from the plant which were immersed after anthesis in 49 °C water for 5 minutes regained self-incompatibility competence during a 48 hour incubation at 24 °C prior to incompatible pollination. Heat treatment of detached pistils as early as 39 hours prior to bud anthesis also resulted in an inactivation of stylar self-incompatibility competence when incompatible pollination was made at 24 hours after anthesis. Experiments utilizing heat treatment of partial lengths of detached whole styles revealed that pollen tubes which have grown through as much at 45 millimeters of either a physiologically incompatible or compatible portion of the style are still capable of shifting to either a higher growth rate or lower growth rate upon entry into respectively either a physiologically compatible or incompatible portion of the style.

HIGH TEMPERATURE‐INDUCED INACTIVATION OF SPOROPHYTIC SELF‐INCOMPATIBILITY IN IPOMOEA FISTULOSA

“…But a 10 min treatment resulted in decreased tube growth, indicating it to be an overdose. Similar observations have been made in Oenothera rhombipetala (Bali, 1963} andLilium longiflorum (Hopper andPeloquin, 1976). Self-compatibility induced by high temperature (50 °C water treatment) in Oenothera organensis was associated with a decrease in the respiratory rate of the stigma (Hodgdon and Hecht, 1975), indicating a change in the physiology of treated pistils.…”

Ploidy manipulation of the gametophyte, endosperm and sporophyte in nature and for crop improvement: a tribute to Professor Stanley J. Peloquin (1921–2008)

“…Research by Dr Peloquin's students included the influence of age and temperature on incompatible and compatible pollen tube growth in Easter lily Peloquin, 1966a, b, 1970). They found inactivation of self-incompatibility following temperature pretreatments of styles (Hopper et al, 1967;Hopper and Peloquin, 1976), and X-ray inactivation of the stylar component of the self-incompatibility reaction (Hopper and Peloquin, 1968).…”