Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS

Wang, Peipei; Zhao, Xiaoliang; Lv, Yanna; Liu, Yanan; Lang, Yinzhi; Wu, Jiandong; Liu, Xin; Li, Miaomiao; Yu, Guangli

doi:10.1016/j.carbpol.2012.05.084

Cited by 51 publications

(17 citation statements)

References 22 publications

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“…Thus, the structures of these oligosaccharides were proved to be a linear sequence with repeating disaccharide units of alternating 1,4-linked GlcA and 1,2-linked Man, as -(4GlcAβ1→2Manα1) m -. This kind of structure was also found in other brown algae [27,28,32,33] and had been reported before.…”

Section: Sequence Analysis Of Heterogeneous Oligosaccharides Preparedsupporting

confidence: 85%

“…Therefore, we can confirm that our fucoidan SFKW was similar, composed of the backbone of a repeating disaccharide unit -(4GlcAβ1→2Manα1) m -with 2,4-O-disulfated and/or 4-O-sulfated fucose oligosaccharides as branches, and all the oligosaccharides were linked to the C3-OH of mannose residues on the backbone. Downloaded by [University of Otago] at 07:05 18 July 2015 It is worth mentioning that the backbone chain with the repeating disaccharide unit -(4GlcAβ1→2Manα1) m -was widely found in brown algae, such as H. fusiforme, [27] S. japonica, [28] and K. crassifolia. [33] Actually, carbohydrates with disaccharide repeating units are ubiquitous not only in animal glycosaminoglycans, such as heparin, heparin sulfate, hyaluronic acid, and chondroitin sulfate A, B, C, D, and E, but also in marine red algae, such as carrageenan and agarose.…”

Section: Sequence Analysis Of Heterogeneous Oligosaccharides Preparedmentioning

confidence: 99%

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Structural Study of Sulfated Fuco-Oligosaccharide Branched Glucuronomannan fromKjellmaniella crassifoliaby ESI-CID-MS/MS

Liu

et al. 2015

Journal of Carbohydrate Chemistry

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303Downloaded by [University of Otago] at 07:05 18 July 2015 304 J. Wu et al.A polysaccharide was purified from brown alga Kjellmaniella crassifolia, and its precise structure was deduced on the basis of sequence analysis of oligosaccharide fragments obtained from mildly and strongly acidic hydrolysis of the polysaccharide. Electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) was used to identify the structural features of the resultant oligosaccharides. The side chain oligosaccharides were composed of α-1,3-linked 4-O-sulfated fucose and α-1,3-linked 2,4-O-disulfated fucose, while the backbone oligosaccharides consisted of the disaccharide repeating unit of β-1,4-linked glucuronic acid and α-1,2-linked mannose. Thus, the fucoidan was determined to be a sulfated fuco-oligosaccharide branched glucuronomannan.

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Section: Sequence Analysis Of Heterogeneous Oligosaccharides Preparedsupporting

confidence: 85%

Section: Sequence Analysis Of Heterogeneous Oligosaccharides Preparedmentioning

confidence: 99%

Structural Study of Sulfated Fuco-Oligosaccharide Branched Glucuronomannan fromKjellmaniella crassifoliaby ESI-CID-MS/MS

Liu

et al. 2015

Journal of Carbohydrate Chemistry

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“…The edible brown alga, H. fusiforme, contains various bioactive compounds and has a long history as a food and medical ingredient. H. fusiforme is especially rich in sulfated polysaccharides, which have various health benefits (Wang et al 2012). Dobashi et al isolated sulfated polysaccharides from H. fusiforme and evaluated their anticoagulant activity (Dobashi et al 1989).…”

Section: Resultsmentioning

confidence: 99%

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

Wang

Yang

et al. 2019

Mar Life Sci Technol

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Our previous study evaluated the in vitro and in vivo antioxidant activities of sulfated polysaccharides from a Celluclastassisted extract of Hizikia fusiforme (HFPS). The results indicate that HFPS possesses potent antioxidant activity and suggest the potential use of HFPS to combat photoaging. In this study, we investigated the ultraviolet (UV) protective effect of HFPS in vitro in keratinocytes (HaCaT cells) and in vivo in zebrafish. The results indicate that HFPS significantly reduced the level of intracellular reactive oxygen species (ROS) and improved the viability of UVB-irradiated HaCaT cells. In addition, HFPS remarkably decreased apoptosis formation in UVB-irradiated HaCaT cells in a dose-dependent manner. The in vivo test results also demonstrate that HFPS significantly reduced intracellular ROS levels, cell death, NO production, and lipid peroxidation levels in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that HFPS possesses strong in vitro and in vivo UV-protective effects, making it a potential ingredient in the cosmeceutical industry.

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“…The fucoidan KW was extracted from brown algae Kjellmaniella crassifolia following the methods described previously21. The average molecular weight of KW determined by gel filtration chromatography was about 536 kDa (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Influenza A Virus Infection by Fucoidan Targeting Viral Neuraminidase and Cellular EGFR Pathway

Wang

Zhang

et al. 2017

Sci Rep

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Development of novel anti-influenza A virus (IAV) drugs with high efficiency and low toxicity is critical for preparedness against influenza outbreaks. Herein, we investigated the anti-IAV activities and mechanisms of fucoidan in vitro and in vivo. The results showed that a fucoidan KW derived from brown algae Kjellmaniella crassifolia effectively blocked IAV infection in vitro with low toxicity. KW possessed broad anti-IAV spectrum and low tendency of induction of viral resistance, superior to the anti-IAV drug amantadine. KW was capable of inactivating virus particles before infection and blocked some stages after adsorption. KW could bind to viral neuraminidase (NA) and inhibit the activity of NA to block the release of IAV. KW also interfered with the activation of EGFR, PKCα, NF-κB, and Akt, and inhibited both IAV endocytosis and EGFR internalization in IAV-infected cells, suggesting that KW may also inhibit cellular EGFR pathway. Moreover, intranasal administration of KW markedly improved survival and decreased viral titers in IAV-infected mice. Therefore, fucoidan KW has the potential to be developed into a novel nasal drop or spray for prevention and treatment of influenza in the future.

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Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS

Cited by 51 publications

References 22 publications

Structural Study of Sulfated Fuco-Oligosaccharide Branched Glucuronomannan fromKjellmaniella crassifoliaby ESI-CID-MS/MS

Structural Study of Sulfated Fuco-Oligosaccharide Branched Glucuronomannan fromKjellmaniella crassifoliaby ESI-CID-MS/MS

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

Inhibition of Influenza A Virus Infection by Fucoidan Targeting Viral Neuraminidase and Cellular EGFR Pathway

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