Inhibition of Influenza A Virus Infection by Fucoidan Targeting Viral Neuraminidase and Cellular EGFR Pathway

Wang, Wei; Wu, Jiandong; Zhang, Xiaoshuang; Hao, Cui; Zhao, Xiaoliang; Jiao, Guangling; Shan, Xindi; Tai, Wenjing; Yu, Guangli

doi:10.1038/srep40760

Cited by 105 publications

(87 citation statements)

References 46 publications

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“…To explore whether compound 5d had direct inhibitory effects on viral particles, plaque reduction assays were performed as described previously (Wang et al, ). As shown in Figure g,h, pre‐incubation of the HSV‐1 or HSV‐2 with compound 5d at concentrations of 1.25–10 μM markedly reduced the number of plaques, suggesting that compound 5d may be able to interact with viral particles directly.…”

Section: Resultsmentioning

confidence: 99%

Guanidine modifications enhance the anti‐herpes simplex virus activity of (E,E)‐4,6‐bis(styryl)‐pyrimidine derivatives in vitro and in vivo

Wang

Zhang

et al. 2020

British J Pharmacology

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Background and Purpose The worldwide prevalence of herpes simplex virus (HSV) and shortage of efficient therapeutic strategies to counteract it are global concerns. In terms of treatment, the widely utilized anti‐HSV drugs such as acyclovir have serious limitations, for example, drug resistance and side effects. Here, we have identified the guanidine‐modified (E,E)‐4,6‐bis(styryl)‐pyrimidine (BS‐pyrimidine) derivative compound 5d as an inhibitor of HSV and further elucidated the anti‐HSV mechanisms of compound 5d both in vitro and in vivo. Experimental Approach Cytopathic effect inhibition assay, plaque assay, and immunofluorescence assay were used to evaluate the anti‐HSV effects of compound 5d in vitro. Membrane fusion assays, immunofluorescence assays, Western blotting assays, and pull‐down assays were used to explore the anti‐HSV mechanisms of compound 5d. HSV‐1‐infected mice, combined with haematoxylin–eosin staining and quantitative RT‐PCR, were used to study the anti‐HSV effects of compound 5d in vivo. Key Results The guanidine‐modified compound 5d rather than the un‐modified compound 3a effectively inhibited both HSV‐1 and HSV‐2 multiplication in different cell lines, more effectively than acyclovir. Compound 5d may block virus binding and post‐binding processes such as membrane fusion, by targeting virus gB protein. In addition, compound 5d may also down‐regulate the cellular PI3K/Akt signalling pathway to interfere with HSV replication. Treatment with compound 5d also markedly improved survival and decreased viral titres in HSV‐infected mice. Conclusions and Implications Thus, the guanidine‐modified BS‐pyrimidine derivatives have the potential to be developed into novel anti‐HSV agents targeting both virus gB protein and cellular PI3K/Akt signalling pathways.

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Section: Resultsmentioning

confidence: 99%

Guanidine modifications enhance the anti‐herpes simplex virus activity of (E,E)‐4,6‐bis(styryl)‐pyrimidine derivatives in vitro and in vivo

Wang

Zhang

et al. 2020

British J Pharmacology

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“…The hemagglutination (HA) assay was performed as previously reported [17]. Standardized chicken red blood cell (cRBC) solutions were prepared according to the WHO manual.…”

Section: Hemagglutination (Ha) Assaymentioning

confidence: 99%

“…The influenza neuraminidase inhibitor detection kit was used to measure the inhibition of NA activity [17]. Briefly, inactivated PR8 virus supernatants was added to a 96-well plate and then mixed with different compounds (diluted in 33 mM MES buffer (pH 3.5), 4 mM CaCl 2 ) at 37°C for 30 min.…”

Section: Neuraminidase Inhibition Assaymentioning

confidence: 99%

“…Furthermore, the inhibition effects of PA on IAV infection was also examined over multiple cycles of infection using plaque reduction assay [17]. Briefly, MDCK cells were infected with PA pretreated virus (Vir09, NWS, and PR8) at an moi of 0.001 pfu for 1 h at 37°C, and then subjected to plaque assay.…”

Section: Inhibition Of Influenza a Virus Multiplication In Vitro By Pmentioning

confidence: 99%

“…Moreover, another time course study was also performed to explore which viral stage after adsorption is inhibited by PA as described previously [17]. Briefly, Vir09 (MOI = 1.0)-infected MDCK cells were treated with 25 μg/mL of PA for different time intervals, then the virus multiplication at 24 h p.i.…”

Section: Influence Of Different Treatment Conditions Of Pa On Iav Infmentioning

confidence: 99%

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Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways

Zhang

Wang

et al. 2019

Virol J

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BackgroundPatchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects.MethodsIn this study, the anti-influenza virus (IAV) activities and mechanisms were investigated both in vitro and in vivo. The inhibitory effects of PA against IAV in vitro were evaluated by plaque assay and immunofluorescence assay. The neuraminidase inhibition assay, hemagglutination inhibition (HI) assay, and western blot assay were used to explore the anti-viral mechanisms. The anti-IAV activities in vivo were determined by mice pneumonia model and HE staining.ResultsThe results showed that PA significantly inhibited different IAV strains multiplication in vitro, and may block IAV infection through inactivating virus particles directly and interfering with some early stages after virus adsorption. Cellular PI3K/Akt and ERK/MAPK signaling pathways may be involved in the anti-IAV actions of PA. Intranasal administration of PA markedly improved mice survival and attenuated pneumonia symptoms in IAV infected mice, comparable to the effects of Oseltamivir.ConclusionsTherefore, Patchouli alcohol has the potential to be developed into a novel anti-IAV agent in the future.

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Schisandra chinensis (Turcz.) Baill. polysaccharide inhibits influenza A virus in vitro and in vivo

Guo

et al. 2023

FEBS Open Bio

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Influenza virus is prone to seasonal spread and widespread outbreaks, which pose important challenges to public health security. Therefore, it is important to effectively prevent and treat influenza virus infection. Schisandra polysaccharide (SPJ) is a polysaccharide derived from the fruit of Schisandra chinensis (Turcz.) Baill. In this study, we evaluated the antiviral activity of SPJ in vitro and in vivo, especially against influenza A virus (IAV) infection. By analyzing SPJ structure and monosaccharide composition, the molecular weight of SPJ was determined to be 115.5 KD, and it is composed of galacturonic acid (89.4%), rhamnose (0.8%), galactose (4.4%), arabinose (3.8%) and glucose (1.7%). Immunofluorescence analysis showed that SPJ treatment reduced the positive rate of viral nucleoproteins in cells, indicating that the compound had an inhibitory effect on influenza virus replication. Furthermore, SPJ therapy improved the survival of infected mice. Lung virus titer assays indicated that SPJ treatment significantly reduced viral‐loading in the lung tissue of infected mice, and alleviated the pathological damage caused by influenza virus infection. Moreover, SPJ reduced cytokine expression during influenza virus challenge. In conclusion, SPJ has anti‐influenza virus effects, and may have potential as an anti‐influenza drug candidate in further clinical studies.

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Inhibition of Influenza A Virus Infection by Fucoidan Targeting Viral Neuraminidase and Cellular EGFR Pathway

Cited by 105 publications

References 46 publications

Guanidine modifications enhance the anti‐herpes simplex virus activity of (E,E)‐4,6‐bis(styryl)‐pyrimidine derivatives in vitro and in vivo

Guanidine modifications enhance the anti‐herpes simplex virus activity of (E,E)‐4,6‐bis(styryl)‐pyrimidine derivatives in vitro and in vivo

Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways

Schisandra chinensis (Turcz.) Baill. polysaccharide inhibits influenza A virus in vitro and in vivo

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