Analysis of steroid hormones in effluents of wastewater treatment plants by liquid chromatoraphy–tandem mass spectrometry

Ingrand, Valérie; Herry, Gaëla; Beausse, Johanne; Roubin, Marie-Renée de

doi:10.1016/s0021-9673(03)00770-2

Cited by 119 publications

(62 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The occurrence of these hormones in the environment is receiving considerable attention due to the fact that most of these compounds are endocrine disrupting chemicals (Christiansen et al, 2002;Cui et al, 2006;Zheng et al, 2013;Huang et al, 2016). Several studies have demonstrated that wastewater treatment systems are the main source of estrogens to aquatic environment (Ingrand et al, 2003;Swart & Pool, 2007;Yoon et al, 2012;Belhaj et al, 2014). A reliable analytical technique is necessary for separation, detection and quantification of the estrogens in the environmental matrix such as water.…”

Section: Introductionmentioning

confidence: 99%

Optimized HPLC -UV Method for Separation, Detection and Quantification of Endocrine Disrupting Estrogens in Low Quality Water

Msigala¹,

Mabiki²,

Styrishave³

et al. 2017

IJC

View full text Add to dashboard Cite

Endocrine disrupting estrogens are emerging contaminants in aquatic ecosystems and environment in general. There are no guidelines for routine monitoring of these chemicals, despite the existing evidences of their adverse health effect to living organisms at low concentrations. This study aimed at developing and validating an optimized HPLC-UV method for detection and quantification of estradiol and ethinylestradiol. Isocratic elution was used for separation and detection of ethinylestradiol and estradiol. The mobile phase was applied with A; water B; acetonitrile (50:50) at flow rate of 0.7mL/min and injection volume 10mL. The precision and accuracy of the method were within the acceptable range. Relative standard deviation of peak area for E2 ranged from 1.373 to 3.668%, and for EE2 ranged from 0.829 to 6.495 %. The percentage recovery for E2 ranged from 82.3 to 99.84 %, and for EE2 ranged from 84.6 to 103.52 %. Linearity of the method was realized at range of 2.5 to 50 ng/mL and 100 to 1000 ng/mL for both E2 and EE2. The linear regression coefficients were 0.9979 and 0.9973 for E2 whereas for EE2 were 0.9983 and 0.9976. Limit of detection were found to be 0.05 ng/mL and 0.08 ng/mL for E2 and EE2 respectively. The obtained limits of quantification were 0.18 and 0.28 ng/mL for E2 and EE2 respectively. In untreated sewage the concentrations of E2 and EE2 were 0.28 ng/ml and 0.18 ng/ml respectively. But in subsequent wastewater stabilization ponds the concentrations were below detection limit. Therefore, the optimized HPLC-UV method is suitable for detection and quantification of endocrine disrupting estrogens when a level of pollution is at least 0.15 ng/ml. At low extent of pollution would require use of the method in conjunction with ELISA technique.

show abstract

Section: Introductionmentioning

confidence: 99%

Optimized HPLC -UV Method for Separation, Detection and Quantification of Endocrine Disrupting Estrogens in Low Quality Water

Msigala¹,

Mabiki²,

Styrishave³

et al. 2017

IJC

View full text Add to dashboard Cite

show abstract

“…GC/MS, [10][11][12] LC/MS 12,13 and LC/MS/MS 12,14 are among the most reliable methods for the determination of estrogens at very low concentration although they may be slightly inferior in terms of sensitivity as compared with an immunoassay, and the instruments are very expensive. On the other hand, enzyme immunoassays are able to sensitively and selectively measure estrogens and can treat a great number of samples at the same time.…”

mentioning

confidence: 99%

Quantitative Analysis of 17β-Estradiol in River Water by Fluorometric Enzyme Immunoassay Using Biotinylated Estradiol

et al. 2005

View full text Add to dashboard Cite

show abstract

“…PPCPs completely from effluents of WWTPs (Ingrand et al, 2003;Esperanza et al, 2004;Sui et al, 2010;Lacey et al, 2011;Ryu et al, 2011). The typical rate of removal of analgesics, anti-inflammatories and beta-blockers are 30% to 40%.…”

Section: Why Do We Need To Monitor Them?mentioning

confidence: 99%

“…High performance liquid chromatography mass spectrometry has been shown as a valuable alternative for detection of PPCPs and EDCs to overcome the drawbacks of GC-MS (Gardinali and Zhao, 2002;Gentili et al, 2002;Ingrand et al, 2003;Cahill et al, 2004;Castiglioni et al, 2005;Schlüsener and Bester, 2005;Martnez Bueno et al, 2007;Gros et al, 2009;Laven et al, 2009;Huerta-Fontela et al, 2010;Jian-lin et al, 2010). Electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) are the three most common ionization techniques coupled with liquid chromatography (Marchi et al, 2009).…”

Section: Ionization Techniquesmentioning

confidence: 99%

“…Therefore, it is not surprising that most of the studies using ESI are focused on the most polar, easily ionizable pharmaceuticals. Only a handful of studies have tried to detect steroid hormones that are difficult to ionize by ESI or APCI with marginal results (Jeannot et al, 2002;Ingrand et al, 2003). Not surprisingly, there is abundant literature for compounds ameanable to ESI but reports are scarce for those that present an ionization challenge.…”

Section: Ionization Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of the Occurrence and Potential Effects of Pharmaceuticals and Personal Care Products in South Florida Waters and Sediments

Wang¹

View full text Add to dashboard Cite

All rights reserved.iv DEDICATION It is not easy to start pursuing a Ph.D and it becomes even harder towards the end. It is a kind of job that makes me start to question myself; it is a period of time in my life that I am desperate to call for help. But I am indeed blessed.I dedicate this dissertation to my parents, without their understanding and support and most of all love, it is impossible for me to walk so far on my way of pursuing my dream. They not only gave me life but also taught me how to become a better person.I dedicate this dissertation to my husband, without him, I will never know the colorful and beautiful part of life. He lets me feel peaceful and powerful when I face difficulties. An ASE-SPE-GC-MS method was developed and applied to the analysis of the sediment and soil area where reclaimed water was used for irrigation. Most analytes were below detection limits, even though some of analytes were detected in the reclaimed water at relatively high concentrations corroborating the fact that PPCPs do not significantly partition to mineral phases.

show abstract

Analysis of steroid hormones in effluents of wastewater treatment plants by liquid chromatoraphy–tandem mass spectrometry

Cited by 119 publications

References 10 publications

Optimized HPLC -UV Method for Separation, Detection and Quantification of Endocrine Disrupting Estrogens in Low Quality Water

Optimized HPLC -UV Method for Separation, Detection and Quantification of Endocrine Disrupting Estrogens in Low Quality Water

Quantitative Analysis of 17β-Estradiol in River Water by Fluorometric Enzyme Immunoassay Using Biotinylated Estradiol

Assessment of the Occurrence and Potential Effects of Pharmaceuticals and Personal Care Products in South Florida Waters and Sediments

Contact Info

Product

Resources

About