Analysis of splenic Gr‐1<sup>int</sup> immature myeloid cells in tumor‐bearing mice

Yamamoto, Yoshiko; Ishigaki, Hirohito; Ishida, Hideyuki; Itoh, Yasushi; Noda, Yoichi; Ogasawara, Kunio

doi:10.1111/j.1348-0421.2008.00009.x

Cited by 22 publications

(24 citation statements)

References 19 publications

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“…ϩ cells into antigen-presenting cells agrees with other results in tumor-bearing mice (73) and polymicrobial sepsis (19). This ex vivo preference appears to be due to the action of GM-CSF, and the cells do not survive in culture without the growth factor.…”

Section: Cd11bsupporting

confidence: 79%

Phenotypic, Morphological, and Functional Heterogeneity of Splenic Immature Myeloid Cells in the Host Response to Tularemia

et al. 2012

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ABSTRACTRecent studies have linked accumulation of the Gr-1+CD11b+cell phenotype with functional immunosuppression in diverse pathological conditions, including bacterial and parasitic infections and cancer. Gr-1+CD11b+cells were the largest population of cells present in the spleens of mice infected with sublethal doses of theFrancisella tularensislive vaccine strain (LVS). In contrast, the number of T cells present in the spleens of these mice did not increase during early infection. There was a significant delay in the kinetics of accumulation of Gr-1+CD11b+cells in the spleens of B-cell-deficient mice, indicating that B cells play a role in recruitment and maintenance of this population in the spleens of mice infected withF. tularensis. The splenic Gr-1+CD11b+cells in tularemia were a heterogeneous population that could be further subdivided into monocytic (mononuclear) and granulocytic (polymorphonuclear) cells using the Ly6C and Ly6G markers and differentiated into antigen-presenting cells followingex vivoculture. Monocytic, CD11b+Ly6ChiLy6G−cells but not granulocytic, CD11b+Ly6CintLy6G+cells purified from the spleens of mice infected withF. tularensissuppressed polyclonal T-cell proliferation via a nitric oxide-dependent pathway. Although the monocytic, CD11b+Ly6ChiLy6G−cells were able to suppress the proliferation of T cells, the large presence of Gr-1+CD11b+cells in mice that survivedF. tularensisinfection also suggests a potential role for these cells in the protective host response to tularemia.

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Section: Cd11bsupporting

confidence: 79%

Phenotypic, Morphological, and Functional Heterogeneity of Splenic Immature Myeloid Cells in the Host Response to Tularemia

et al. 2012

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“…In other models it was observed that the accumulation of CD11b + Gr-1 + in the spleen has been associated with tumor development. Yamamoto et al [19] evaluated CD11b + Gr-1 + expression in splenocytes of the BALB/c mice inoculated with colon carcinoma and sarcoma cell lines by the time the tumors reached 10 mm in diameter. It was found that the naive BALB/c mice presented 4.4% of CD11b + Gr-1 + in the spleen whereas tumor-bearing mice expressed more than 11% of this suppressor cell marker.…”

Section: Discussionmentioning

confidence: 99%

Identification of myeloid-derived suppressor cells and T regulatory cells in lung microenvironment after Urethane-induced lung tumor

Rosin

Pedregosa

Almeida

et al. 2011

International Immunopharmacology

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“…These two subpopulations diVer in their potential to suppress T-cell responses [14]. The application of Ly-6C-and Ly-6G-speciWc antibodies revealed two diVerent subpopulations of MDSC in spleens of tumor-bearing mice: CD11b + Ly6G + Ly6C low MDSC morphologically resembling polymorphonuclear granulocytes (PMN-MDSC) and CD11b + Ly6G -Ly6C high MDSC with monocytic phenotype (MO-MDSC) [15].…”

Section: Mdsc Surface Markers and Subsetsmentioning

confidence: 99%

Subsets, expansion and activation of myeloid-derived suppressor cells

Ribechini

Greifenberg

Sandwick

et al. 2010

Med Microbiol Immunol

158

142

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Tumor cells and microorganisms manipulate the immune system to minimize any counter response in order to survive. Myeloid-derived suppressor cells (MDSC) in the mouse represent activated Gr-1(+) CD11b(+) myeloid precursor cells. Activation may occur through endogenous or exogenous factors leading to the suppression of immune responses. Under steady state conditions the same precursors differentiate into dendritic cells, macrophages and neutrophils. Their linkage to tumor progression and several suppression mechanisms employing the arginine metabolism are well documented, but knowledge of their role in chronic infections, autoimmune diseases and graft-versus-host reactions is just emerging. Several factors have been described to promote MDSC expansion and activation in bone marrow, spleen and tumor sites. New evidence suggests that the Gr-1 antibody itself may differentially trigger myelopoiesis under steady state conditions or induce apoptosis in inflammatory situations after binding to a common epitope expressed on Ly-6C and Ly-6G molecules, respectively. Moreover, two subsets of neutrophil- and monocyte-related MDSC have been described in tumor-bearing and healthy mice. In the present review, we summarize some early work leading to recent findings on these two MDSC subsets, the factors supporting MDSC expansion and activation, as well as novel insights on Gr-1 antibody functions.

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Analysis of splenic Gr‐1^int immature myeloid cells in tumor‐bearing mice

Cited by 22 publications

References 19 publications

Phenotypic, Morphological, and Functional Heterogeneity of Splenic Immature Myeloid Cells in the Host Response to Tularemia

Phenotypic, Morphological, and Functional Heterogeneity of Splenic Immature Myeloid Cells in the Host Response to Tularemia

Identification of myeloid-derived suppressor cells and T regulatory cells in lung microenvironment after Urethane-induced lung tumor

Subsets, expansion and activation of myeloid-derived suppressor cells

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Analysis of splenic Gr‐1int immature myeloid cells in tumor‐bearing mice

Cited by 22 publications

References 19 publications

Phenotypic, Morphological, and Functional Heterogeneity of Splenic Immature Myeloid Cells in the Host Response to Tularemia

Phenotypic, Morphological, and Functional Heterogeneity of Splenic Immature Myeloid Cells in the Host Response to Tularemia

Identification of myeloid-derived suppressor cells and T regulatory cells in lung microenvironment after Urethane-induced lung tumor

Subsets, expansion and activation of myeloid-derived suppressor cells

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Analysis of splenic Gr‐1^int immature myeloid cells in tumor‐bearing mice