Analysis of Spermatozoa Quality Using Percoll Density Gradient Centrifugation Through the Administration of Phospholipid + Egta

Rumende, Rooije R.H.; Baideng, Eva L.; Rares, Fredine E. S.; Rares, Laya M.

doi:10.46909//journalalse-2021-026

Cited by 2 publications

(2 citation statements)

References 23 publications

(16 reference statements)

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“…The results of this study are supported by Rumande et al [52] and that an increase in membrane integrity will greatly affect motility. This increase in motility is due to the influence of fat contained in the plasma membrane in the form of ATP as an energy source.…”

Section: B Discussionsupporting

confidence: 87%

Optimizing Reproductive Performances of Garut Sheep as Superior Local Genetic Resources by Modification of Dietary Grass and Concentrate Balances

Rasad¹,

Ayuningsih²,

Solihati³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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High-quality Garut rams are important to be selected for Garut sheep development to include improving their semen qualities. The semen quality is highly affected by dietary nutrient intake. This research aimed to conclude the effect of different grass and concentrate balances on performance and semen qualities such as motility, abnormality, the integrity of plasma membrane, and acrosome cap sperms of Garut rams. This research was conducted from August 25th, 2020, to February 2nd, 2021, at Margawati Sheep Breeding Center. A Completely Randomized Design (CRD) was used to compare 3 different grass and concentrate balance treatments (T1 = 80% grass: 20% concentrate; T2 = 60% grass: 40% concentrate; and T3 = 40% grass: 60% concentrate) on performance and semen qualities of Garut rams using 6 replicates (n = 6). The data were analyzed using analysis of variance and Duncan's test. The results showed there was no different among the treatments on average daily gain (ADG, g/head/day) and dry matter intake (DMI, g/head/day) but increasing level of dietary concentrate resulted in increased motility (P<0.01), reduced abnormality (P<0.05), increased integrity of plasma membrane (P<0.001) and increased acrosome cap (P<0.001) of sperms of Garut Rams. The T2 treatment resulted in an optimal grass (60%) and concentrate (40%) balance. In conclusion, increased dietary concentrate did not significantly increase the daily weight gain, but it improved the Garut ram's semen quality.

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Section: B Discussionsupporting

confidence: 87%

Optimizing Reproductive Performances of Garut Sheep as Superior Local Genetic Resources by Modification of Dietary Grass and Concentrate Balances

Rasad¹,

Ayuningsih²,

Solihati³

et al. 2022

International Journal on Advanced Science, Engineering and Information Technology

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show abstract

“…Bagian T1 akan diterapkan perlakuan sentrifugasi dengan 2500rpm selama 5 menit, setelah sentrifugasi akan diambil menjadi tiga ulangan, setiap ulangan dengan 1 tetes sampel pada gelas objek yang akan ditetesi 1 tetes larutan eosin (pewarna) dan langsung diuji mikroskopis dibawa mikroskop dengan perbesaran 400x dan 1000x. Bagian T2 akan diterapkan perlakuan sentrifugasi dengan 2500rpm selama 5 menit dan penambahan kuning telur konsentrasi 5% (5% dari banyaknya sampel yang didapat, setelah sentrifugasi akan diambil menjadi tiga ulangan, setiap ulangan dengan 1 tetes sampel pada gelas objek yang akan ditetesi 1 tetes larutan eosin (pewarna) dan langsung diuji mikroskopis dibawa mikroskop dengan perbesaran 400x dan 1000x (Rumende, 2021).…”

Section: Pemeriksaan Mikroskopis Yang Meliputi Parameter Penelitianunclassified

Integritas Membran Spermatozoa Manusia pada Proses Sexing dengan Pemberian Kuning Telur

Suoth,

Rumende,

Papu

2023

JBL

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Sexing atau pemisahan spermatozoa adalah proses pemisahan spermatozoa yang membawa sifat kelamin jantan dan betina. Infertilitas merupakan satu kondisi dimana pasangan yang aktif secara seksual tanpa melakukan kontrasepsi tidak mampu mendapat kehamilan dalam kurun waktu selama 1 tahun. Sekitar 50-80 juta pasang suami istri di dunia (1 dari 7 pasutri) yang terkena masalah infertil dan pada setiap tahun muncul sekitar 2 juta pasangan suami istri infertil. Di Indonesia diperkirakan ada 4 juta sekian pasangan suami istri yang mengalami infertil atau ketidaksuburan. Solusi yang dapat dilakukan untuk mencegah dan menghindari kerusakan membran pada spermatozoa saat pemisahan spermatozoa adalah dengan menggunakan metode sentrifugasi dengan penambahan kuning telur. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis integritas membran spermatozoa pada proses sexing sebelum dan sesudah pemberian kuning telur. Metode yang digunakan adalah Rancangan Acak Lengkap dengan tiga perlakuan, yaitu kontrol, sentrifugasi, sentrifugasi + kuning telur. Hasil penelitian menunjukkan bahwa integritas membran yang termasuk destabilisasi membran dan disintegritas pada kontrol sebesar 18% dan 19%. Persentase perlakuan sentrifugasi yang termasuk destabilisasi membran dan disintegritas sebesar 57% dan 50%. Sedangkan persentase perlakuan sentrifugasi + kuning telur yang termasuk destabilisasi membran dan disintegritas sebesar 25% dan 31%. Perbedaan yang signifikan terlihat pada perlakuan sentrifugasi terhadap perlakuan yang lain.

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Analysis of Spermatozoa Quality Using Percoll Density Gradient Centrifugation Through the Administration of Phospholipid + Egta

Cited by 2 publications

References 23 publications

Optimizing Reproductive Performances of Garut Sheep as Superior Local Genetic Resources by Modification of Dietary Grass and Concentrate Balances

Optimizing Reproductive Performances of Garut Sheep as Superior Local Genetic Resources by Modification of Dietary Grass and Concentrate Balances

Integritas Membran Spermatozoa Manusia pada Proses Sexing dengan Pemberian Kuning Telur

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