Analysis of sperm function in Kartagener’s syndrome

Aitken, R. John; Ross, Allyson; Lees, M. M.

doi:10.1016/s0015-0282(16)47434-0

Cited by 74 publications

(28 citation statements)

References 7 publications

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“…Although no assessments of the quality of sperm movement were made in this study we have previously shown that the movement characteristics exhibited by human spermatozoa have little impact on the aspects of sperm function measured by the penetration test (Aitken et al, 1983a(Aitken et al, , 1985. The quality of sperm motility is clearly a major factor in determining the ability of human spermatozoa to penetrate the zona pellucida and cervical mucus, both of which are heavily dependent upon the shearing forces generated by sperm motility.…”

Section: Discussionmentioning

confidence: 75%

“…The quality of sperm motility is clearly a major factor in determining the ability of human spermatozoa to penetrate the zona pellucida and cervical mucus, both of which are heavily dependent upon the shearing forces generated by sperm motility. In contrast, fusion between the vitelline membrane of the hamster oocyte and the human spermatozoon is dependent on gamete contact (Aitken et al, 1983a) rather than any specific attribute of sperm motility.…”

Section: Discussionmentioning

confidence: 89%

“…Since this procedure does not examine certain aspects of sperm function, such as penetration of cervical mucus (Aitken, Sutton, Warner & Richardson, 1985) or the competence of human spermatozoa to interact with the zona pellucida (Gould et al, 1983), false positive results are to be expected (Overstreet et al, 1980;Aitken, Ross & Lees, 1983a). However, because the major property assessed by the penetration assay, sperm-oocyte fusion (Aitken et al, 1986), is a critical component of the fertilization process, poor or negative results should be of prognostic value (Aitken et al, , 1986.…”

Section: Discussionmentioning

confidence: 99%

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Application of Poisson distribution theory to the zona-free hamster oocyte penetration test to assess sperm function of men with asthenozoospermia

Aitken¹,

Elton²

1986

Reproduction

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Summary. Assessments of the penetrating potential of human spermatozoa were carried out using the zona-free hamster oocyte penetration test on 4 groups of subjects exhibiting (1) normal fertility, (2) idiopathic asthenozoospermia(< 40% motility), (3) asthenozoospermia associated with varicocoele and (4) oligoasthenozoospermia (<20 \m=x\106 spermatozoa/ml and <40% motility). When the Poisson model was used to correct the results of the in-vitro penetration experiments for differences in motile sperm concentration, significant differences were apparent between the normal fertile controls and all 3 categories of asthenozoospermic patient. Furthermore, the penetrating ability of the motile spermatozoa from patients presenting with a varicocoele or oligoasthenozoospermia was significantly less than that for the group in which asthenozoospermia was the only detectable defect. These results emphasize the practical significance of the Poisson model in the analysis of male fertility and demonstrate that the asthenozoospermic condition is associated with a significant reduction in the fertilizing potential of the motile spermatozoa.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Application of Poisson distribution theory to the zona-free hamster oocyte penetration test to assess sperm function of men with asthenozoospermia

Aitken¹,

Elton²

1986

Reproduction

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“…In contrast, it has been reported that the abnormal axoneme is induced by hereditary disease as in the Wobbler mouse and Kartagener's syndrome (LEESTMA and SEPSENWOL, 1980;AFZELIUS, 1981;AITKEN et al, 1983). Judging from extremely rare cases involving the abnormal axoneme, it is considered that the abnormal axoneme may be controlled by hereditary factors rather than other non-hereditary ones, while the periaxonemal components are controlled by other factors.…”

Section: Discussionmentioning

confidence: 99%

Flagellum Abnormalities of Spermatozoa in Seminiferous Tubules after a Short Term Vasectomy.

Hamasaki

Miyajima

Murakami

1991

Archives of Histology and Cytology

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“…Clinically, we have demonstrated that motility and the potential for fertilization are independent properties exhibited by spermatozoa, which may be differentially affected in cases of male infertility (Aitken, Ross & Lees, 1983b;Aitken et al, 1983d). As a result, Poisson analysis should find application in a variety of clinical conditions associated with asthenozoospermia, such as oligozoospermia or varicocoele, in permitting analysis of the penetrating ability of the spermatozoa in isolation from the defects in motility.…”

Section: Discussionmentioning

confidence: 99%

Significance of Poisson distribution theory in analysing the interaction between human spermatozoa and zona-free hamster oocytes

Aitken¹,

Elton²

1984

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Summary. The value of Poisson distribution theory in describing and predicting the nature of sperm\p=n-\egg interaction in vitro has been investigated using an interspecific i n\ x=r eq-\ vitro fertilization system, incorporating zona-free hamster oocytes and human spermatozoa. The frequency distribution of polyspermic oocyte penetrations in 72 experiments exhibited good agreement with the Poisson distribution at all levels of fertilization, indicating that each oocyte must be of equal penetrability and that there can be no block to polyspermy in this interspecific system. Poisson distribution theory also accurately described the relationship between oocyte penetration and sperm motility in 50 out of 54 separate experiments spread across 10 serial dilution curves. For each dilution series the shape of the fitted curve was fixed but its location along the x\ x=r eq-\ axis varied from donor to donor. The fixed nature of the relationship between sperm motility and egg penetration enables the results of such in-vitro fertilization experiments to be corrected for the number of motile spermatozoa in the incubation media. On the basis of these findings a protocol is described for assessing the results of the zona-free hamster oocyte penetration assay, which involves analysis of the degree of polyspermy followed by the application of Poisson distribution theory to correct the results to a standard concentration of motile spermatozoa. Changes in the penetrating ability of human spermatozoa after vasectomy and characterization of the degree of inter-ejaculate variation in penetrating potential are two clinical examples of such analyses given in the text. The statistical methods described in this paper should also be of general relevance to the study of fertilization mechanisms, in providing a rationale by which to analyse the quantitative nature of sperm\p=n-\egg interaction in vitro.

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Analysis of sperm function in Kartagener’s syndrome

Cited by 74 publications

References 7 publications

Application of Poisson distribution theory to the zona-free hamster oocyte penetration test to assess sperm function of men with asthenozoospermia

Application of Poisson distribution theory to the zona-free hamster oocyte penetration test to assess sperm function of men with asthenozoospermia

Flagellum Abnormalities of Spermatozoa in Seminiferous Tubules after a Short Term Vasectomy.

Significance of Poisson distribution theory in analysing the interaction between human spermatozoa and zona-free hamster oocytes

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