Analysis of Small Photographic Images of Line Targets

Gilmore, H. F.

doi:10.21236/ad0829216

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…review by GORINI and BECKWITH 1966). Similar results have also been obtained with eucaryotes; e.g., super-suppressors, or multisite suppressors, which have the ability to revert several loci simultaneously, have been known for some time in yeast (HAW-THORNE and MORTIMER 1963;MANNEY 1964;GILMORE 1967).…”

Section: Evidence For Suppression Of Arom Mutant M54 By the Suppressor Am Su22supporting

confidence: 70%

EVIDENCE FOR NONSENSE MUTATIONS IN THE AROM GENE CLUSTER OF NEUROSPORA CRASSA

Case¹,

Giles²

1968

Genetics

View full text Add to dashboard Cite

show abstract

Section: Evidence For Suppression Of Arom Mutant M54 By the Suppressor Am Su22supporting

confidence: 70%

EVIDENCE FOR NONSENSE MUTATIONS IN THE AROM GENE CLUSTER OF NEUROSPORA CRASSA

Case¹,

Giles²

1968

Genetics

View full text Add to dashboard Cite

show abstract

“…Other deleterious effects of yeast ochre suppressors have been reported. These are: the lethal effect of two suppressors in one cell (GILMORE 1967), and the effect of a cytoplasmic factor [psi+] on the class I and class I1 suppressors (Cox 1971). GILMORE and Cox have suggested that under the above conditions, high efficiency ochre suppression causes the translation of natural termination codons resulting in abnormal terminal additions to proteins and impairment of cellular functions.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ochre Suppression on Meiosis and Ascospore Formation in Saccharomyces

Rothstein

Esposito

1977

Genetics

View full text Add to dashboard Cite

The effect of altered tyrosyl-tRNAs on the developmental process of sporulation was examined. Mutations in eight independent loci resulting in tyrosine-inserting nonsense suppressors were tested for their effects on sporulation. Different levels of inhibition were found ranging from SUP3-o, which caused the greatest reduction of sporulation (7-17% of wild type), to SUP11-o which caused no reduction in sporulation. Since the SUP3-o mutation exhibited the greatest effect, it was studied in detail. Although SUP3-o is a dominant nonsense suppressor, its effect on sporulation is recessive. Expression of the sporulation deficiency is dependent upon the stage of transfer from glucose growth medium (i.e., log, early stationary, etc.) to sporulation medium. SUP3-o/SUP3-o diploid cells transferred from log or early stationary phase are capable of sporulation, whereas cells transferred after early stationary phase (i.e., after adaptation to respiration) exhibit poor sporulative ability. Sporulation events were examined under restrictive conditions to observe those events completed by SUP3-o/SUP3-o diploids. The early events of sporulation occur in these cells. Later events are completed by progressively fewer cells. Premeiotic DNA synthesis occurred in approximately 40% of the cells, nuclear segregation occurred in 20%, and finally, only 2% formed asci. The fact that fewer late-sporulation events occur under restrictive conditions can be explained by increased efficiency of suppression.

show abstract

“…In this case, it would not be the absence of an essential function but the oversuppression which is lethal to the haploid cell. Some effect on cell growth when suppression efficiency is enhanced was suggested by GILMORE (1967). By generating a very efficient suppressor mutation, it can be imagined that a diploid could be produced with a recessive lethal phenotype, yielding two live spores and two dead spores.…”

Section: Discussionmentioning

confidence: 99%

Recessive Lethal Amber Suppressors in Yeast

1975

View full text Add to dashboard Cite

Recessive lethal amber suppressor mutations have been isolated in a diploid strain of Saccharomyces cerevisiae. Diploids carrying these suppressors upon sporulation yield asci with only two live spores, both lacking the suppressor. At least two classes of recessive lethal suppressors exist. Aneuploid strains carrying one wild type and one suppressor locus have been isolated and used in mapping studies; one suppressor maps on chromosome III, the other does not.

show abstract

Analysis of Small Photographic Images of Line Targets

Cited by 7 publications

References 0 publications

EVIDENCE FOR NONSENSE MUTATIONS IN THE AROM GENE CLUSTER OF NEUROSPORA CRASSA

EVIDENCE FOR NONSENSE MUTATIONS IN THE AROM GENE CLUSTER OF NEUROSPORA CRASSA

The Effect of Ochre Suppression on Meiosis and Ascospore Formation in Saccharomyces

Recessive Lethal Amber Suppressors in Yeast

Contact Info

Product

Resources

About