EVIDENCE FOR NONSENSE MUTATIONS IN THE <i>AROM</i> GENE CLUSTER OF <i>NEUROSPORA CRASSA</i>

Case, Mary E.; Giles, Norman H.

doi:10.1093/genetics/60.1.49

Cited by 32 publications

(6 citation statements)

References 4 publications

(7 reference statements)

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“…Many mutant hunts have failed to provide such mutations, Alternatively, if transcription or translation were initiated from the me-7 end a nonsense or frame-shift mutation in the me-7 region should remove both functions. One me-7 allele (NM251) behaves like a nonsense mutant of the amber type since it is suppressible by a supersuppressor known to suppress specific CRM-less, non-complementing alleles at the am (amination) locus (Seale, 1968) and certain polar mutations within the arom (aromatic) cluster (Case & Giles, 1968).…”

Section: Methodsmentioning

confidence: 99%

Recombination events that span sites within neighbouring gene loci ofNeurospora

Murray

1970

Genet. Res.

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SUMMARYEvidence is given that me-7 and me-9 are separate but contiguous gene loci.The pattern of polarized recombination throughout the me-7 me-9 region indicates the location of a recombinational discontinuity between the known me-7 alleles and the known me-9 alleles.Recombination events may include sites in both genes but such events are not preferentially associated with parental combinations of flanking markers.Recombination events extending into both genes provide an extra criterion for the ordering of the sites within the me-7 locus. The order so obtained confirms that deduced from the flanking markers of me+ recombinants from me-7 × me-7 crosses.In me-7 × me-9 crosses but not in me-7 × me-7 crosses, the map order derived on the assumption that single exchanges are more frequent than apparent triple exchanges is the reverse of that derived from prototroph frequencies. It is concluded that the former criterion is more likely to have provided the correct order and the anomalous prototroph frequencies reflect the polarity of gene conversion within me-7.

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Section: Methodsmentioning

confidence: 99%

Recombination events that span sites within neighbouring gene loci ofNeurospora

Murray

1970

Genet. Res.

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“…Complementation between alleles that block the same single step has been detected only for aro-2 and aro-J (139,389). Polar mutants are divided into six classes (A through F) based chiefly on their complementation behav-ior (389); types D, E, and F are semicolonial and have yellowish-orange conidia (144). Singlefunction aro-9 mutants were obtained by selecting in a strain of genotype qa-1, which is noninducible for catabolic dehydroquinase activity (885).…”

Section: Aro: Aromaticmentioning

confidence: 99%

“…aro(p) indicates polar mutations in the aro cluster. Noncomplementing alleles M26, M1039, M1065, M1108, M1162, M1172, and Y306M54 (abbreviated M54) are suppressible by nonsense suppressors ("supersuppressors") (144,145,957). aro-1: aromatic-i Specifies shikimate kinase (389) (Fig.…”

Section: Aro: Aromaticmentioning

confidence: 99%

“…Inserts tyrosine in the site where the wild type has glutamate (956). Used to identify suppressible alleles of aro(p), trp-i, trp-2, trp-3 (953,144,183), and ad-3B (749). Although perhaps the most efficient of known ssu mutations, ssu-i restores only about 20%o of the wild-type amount of normal glutamate dehydrogenase in the double mutant with am allele (17) (reference 956).…”

Section: Downloaded Frommentioning

confidence: 99%

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Chromosomal loci of Neurospora crassa

Perkins¹,

Radford²,

Newmeyer³

et al. 1982

Microbiol Rev

259

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I I ' ~~~~T h Ñ CiOr(r r N z ADDITIONAL LOCI IN LINKAGE GROUP ILinked to mt (8 to 12%) Linked to mt, acr-3 (5%) Right of arg-i (5/29) Linked to so and aro-8 Right of un-18 Between ad-5 and the centromere Between arg-i (4%) and his-3 (I to 2%) Between In(H4250) and T(39311) Left of mt (23%) Linked to mt (2%) and aza-i (39%o) Left of nit-2 (30%) Left of mt (14%) Linked to un-i (9%), probably to the right Linked to hom (1%), probably to the right Linked to mt (17 to 22%) Between T(NM103) and al-2 (18%) Between T(4540) and cr-2Between thi-i (12 to 20%/6) and ad-9 (5%) Linked to ad-5 (1/54), probably to the left Linked to mt (6%) Linked to cys-5 (<1%), probably to the right Right of ad-9 (12%), linked to al (0/76) Right of his-3 (2%) Between the T(AR173) breakpoints Linked to al-2 (10%) and nic-i (1 to 5%) Linked to ad-9 (0/44); right of thi-i (2%) Linked to mt (5%), probably to the left Linked to al-I (10%) Right of os-i (3%) Between nit,i (5 to 19%) and al-I (6 to 19%io) Linked near arg-i Left of al-2 (25%o) Left of mt (16%) Right of nic-2 (6%) Linked to ad-9 (2%), probably to the right Left of cr-i (15%) Left of mt (10%) Probably between his-2 (3%) and cr-i (3%) Between T(NM103) and the right tip Between mt (20%) and arg-i (1%) Between ad-3 (6%) and al-i (16%) Between his-3 and nic-2 Between cyh-i (5%) and al-I (7%) Linked to cyh-i (18%), al-i (2%), and mb-2 (6%) Between arg-i (3%) and T(39311) Between tre (41%) and ad-9 Linked to mt (9%) Linked to mt (20%) Complex phenotype. Alternative requirements: 2-acetylaminofluorine, certain azo dyes, or certain single amino acids. Cold sensitive. Originated among progeny of a rib-i strain that had become tolerant to 2-acetylaminofluorine.

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“…SEALE (1968), however, identified one class of revertants from a m (1 7) in which restored GDH activity was due to suppressor mutation in a gene or genes unlinked to am. CASE and GILES (1968) showed that the first suppressor (ssu-I) isolated by SEALE would also suppress several pleiotropic mutants in the arom cluster and subsequent work (summarized by SEALE, CASE and BARRATT 1969;SEALE 1972) identified seven different suppressor genes, designated ssu-I to ssu-7, mutants of which would suppress a range of specific alleles in am, arom, his-3, trp-l and trp-2. Many but not all of these alleles are suppressed by each of the suppressors (CHALMERS and SEALE 1971 ;SEALE 1976).…”

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confidence: 99%

Amino Acid Replacements Resulting From Suppression and Missense Reversion of a Chain-Terminator Mutation in Neurospora

et al. 1977

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The Neurospora crassa super-suppressor mutation, ssu-1, suppresses the auxotrophic phenotype of the mutant am(17) by inserting tyrosine at residue 313 of NADP-specific glutamate dehydrogenase, a position occupied in the wild type by glutamate. Two classes of am(17) revertants due to further mutation within the am gene have, respectively, tyrosine and leucine at residue 313. These replacements are consistent with a chain-terminating codon in am(17) of either the amber (UAG) or the ochre type (UAA), but are inconsistent with UGA. The Leu313 and Tyr313 variants of the enzyme have effective activity but are grossly different from the wild type in Michaelis constants (especially for ammonium) and heat stabilities at two different pH values. They show smaller but significant differences in these respects from each other.

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EVIDENCE FOR NONSENSE MUTATIONS IN THE AROM GENE CLUSTER OF NEUROSPORA CRASSA

Cited by 32 publications

References 4 publications

Recombination events that span sites within neighbouring gene loci ofNeurospora

Recombination events that span sites within neighbouring gene loci ofNeurospora

Chromosomal loci of Neurospora crassa

Amino Acid Replacements Resulting From Suppression and Missense Reversion of a Chain-Terminator Mutation in Neurospora

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