Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Salo, Piia; Salomies, Hannele; Harju, Kirsi; Ketola, Raimo A.; Kotiaho, Tapio; Yli‐Kauhaluoma, Jari; Kostiainen, Risto

doi:10.1016/j.jasms.2005.02.025

Cited by 59 publications

(36 citation statements)

References 41 publications

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“…The authors do not report any plate height measurements. An emerging use of monolithic layers is in mass spectrometry where an increase in mass detection limits of an order or magnitude or more have been obtained using matrix-assisted laser desorption ionization (MALDI) [86,87] and surface desorption electrospray ionization techniques [88] compared with high performance stabilized particle layers. These improvements result mainly from a reduction in the layer thickness rather than an increase in separation performance.…”

Section: Monolithic Filmsmentioning

confidence: 99%

High performance stationary phases for planar chromatography

Poole

2011

Journal of Chromatography A

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Section: Monolithic Filmsmentioning

confidence: 99%

High performance stationary phases for planar chromatography

Poole

2011

Journal of Chromatography A

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“…Thus, it seems reasonable to assume that a reduced thickness of the stationary phase would enhance the detectability of the complete analyte. Thus, standard TLC plates were replaced by ultra-thin-layer chromatography (UTLC) plates (Salo et al 2005) and it could be shown that due to the thinner adsorbent layer the monolithic UTLC plates provide 10-100 times better sensitivities. Although the majority of commercially available MALDI devices use high vacuum conditions, atmospheric pressure (AP) MALDI is increasingly used for two reasons: (a) these devices are less accident-sensitive and (b) provide the capability to record collisionally induced (CI) fragment ion spectra without the need of an extra collision cell.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

Schiller

Fuchs

Teuber

et al. 2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Thin-layer chromatography (TLC) is a widely used, fast and inexpensive method. Even minor constituents of complex mixtures can be often characterized. Unfortunately, the unambiguous assignment of the spots to defined compounds is often difficult because common staining methods normally reveal only (at best) a substance class, not a specific compound. For instance, in the case of phospholipids, the lipid class, but not the detailed fatty acyl composition, can be determined (normal phase TLC). Nowadays mass spectrometry (MS) seems to be the most suitable method for analyte characterization due to its high sensitivity and mass accuracy. This became possible by the invention of soft ionization methods such as matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) MS and electrospray ionization (ESI) although many further desorption techniques are available nowadays. This review summarizes the so far available knowledge about direct TLC/MS couplings and gives an overview about selected compounds that could be successfully analyzed. Chromatographic Methods and Product IdentificationLiquid chromatographic methods, especially high-performance liquid chromatography (HPLC) and planar chromatography [in particular thin-layer (TLC) or highperformance thin-layer chromatography (HPTLC)], are indispensable tools of modern analytical chemistry (Hahn-Deinstrop 2006 l TLC is a very robust system and to a much lesser extent affected by impurities potentially present in the sample. Thus, TLC may also be used for the analysis of "suspicious" samples (for instance, crude tissue or food extracts) that might easily damage or plug the column of an HPLC system. l HPLC columns are normally rather expensive and are, thus, many times reused subsequent to careful washing to remove previous analytes. However, it can never be completely ruled out that there is some residual material on the column. Thus, it is often more difficult to certify an HPLC assay than a TLC assay because TLC does not provide any "memory" effects as a completely new stationary phase is used in all cases. The consumption of solvents is much lower than in the case of HPLC. This makes TLC less expensive and particularly less harmful and more environment friendly.l Although nowadays there are also "multiplexing" HPLC systems available (Woodbury et al. 1995) that allow the simultaneous analysis of several samples in parallel, the simple analysis of a multitude of samples is also a clear advantage of TLC.There is hardly a class of small molecules that have not yet been analyzed by TLC, and thus this review will not deal with improvements of the achievable separation quality, neither by optimizing the stationary phase nor the solvent system nor sample application.However, separation is only one (unequivocally very important) step of successful analysis, and the detection of the different analytes on the TLC plate and their structural elucidation is also very important. This particularly holds because the retardation factor (R f value) by which a com...

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“…Moreover, the TLC plate size is not limited by the dimensions of a vacuum chamber. Techniques, such as electrospray ionization (ESI) [17], [18], [19], electrospray-assisted laser desorption ionization (ELDI) [20], desorption electrospray ionization (DESI) [21], laser desorption/atmospheric pressure chemical ionization (LD-APCI) [22], atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) [23], [24] and direct analysis in real time (DART) [25], [26] have been adopted to generate mass spectra of compounds directly from TLC plates.…”

Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates

et al. 2014

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A new method for on-spot detection and characterization of organic compounds resolved on thin layer chromatography (TLC) plates has been proposed. This method combines TLC with dielectric barrier discharge ionization (DBDI), which produces stable low-temperature plasma. At first, the compounds were separated on TLC plates and then their mass spectra were directly obtained with no additional sample preparation. To obtain good quality spectra the center of a particular TLC spot was heated from the bottom to increase volatility of the compound. MS/MS analyses were also performed to additionally characterize all analytes. The detection limit of proposed method was estimated to be 100 ng/spot of compound.

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Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry

Cited by 59 publications

References 41 publications

High performance stationary phases for planar chromatography

High performance stationary phases for planar chromatography

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates

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