Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry

Zhao, Jingtong; Liu, Meng; Shi, Tongfei; Gao, Mingyu; Lv, Yuqian; Zhao, Yawei; Li, Jing; Zhang, Ming; Zhang, Hansi; Guan, Fengying; He, Kan; Chen, Li

doi:10.3390/molecules26237181

Cited by 9 publications

(19 citation statements)

References 40 publications

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“…Based on this technology, our study found that the levels of Glutathione (GSH), Ornithine, Aminoadipic acid, Alpha-dimorphecolic acid, Carnosine (CA), and Anserine in KOA rats were return to normal after either T or S treatment. Previous research (35) suggested possible endogenous metabolite biomarkers of the development and progression of KOA, also including Aminoadipic acid, which was partially consistent with our results.…”

Section: Discussionsupporting

confidence: 93%

Metabonomics study on the effects of acupuncture and moxibustion in a rat knee osteoarthritis model

Li,

Tan,

Liang

et al. 2023

Preprint

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For elucidating effects of acupuncture and moxibustion on the metabolism of knee osteoarthritis (KOA) in rats, A method for metabolite analysis based on high throughput liquid chromatography-tandem mass spectrometry (LC-MS) was used to investigate overall changes in serum between groups. Rats were divided into four groups: control (C), model (M), KOA plus traditional acupuncture and moxibustion (T) and KOA plus syndrome differentiation acupuncture and moxibustion (S). The paw withdrawal threshold, open-field test and Hematoxylin and eosinstaining were applied to assess the transformations of knee hyperalgesia and histopathology. The serum metabolic fingerprints were obtained by using metabonomics. Orthogonal partial least squares-discriminant analysis was evaluated to highlight the differences and identify possible biomarkers. The changing trends of potential serum biomarkers and biological pathways of differential metabolites among different groups were analyzed. The results showed that acupuncture and moxibustion alleviate hyperalgesia and cartilage degeneration in KOA rats. A total of 11 metabolites were annotated between C and M. Among the 11 metabolites, the level of 5 metabolites were reversed in group S, including Glutathione, Ornithine, Alpha-dimorphecolic acid, Carnosine, Anserine, and group T only reversed the contents of 2 metabolites, including Aminoadipic acid and Carnosine. T versus M, beta-Alanine metabolism, Histidine metabolism, Tryptophan metabolism, mTOR signaling pathway, and Chagas disease were the affected pathways. Therefore, beta-Alanine metabolism, Histidine metabolism, Tryptophan metabolism were the main differential metabolic pathways after T treatment. In the comparison of the S and M groups, beta-Alanine metabolism, mTOR signaling pathway, D-arginine and D-ornithine metabolism, PPAR signaling pathway, and Chagas disease were the affected pathways. In conclusion, acupuncture and moxibustion can ameliorate the symptoms of KOA. Compared with traditional acupuncture and moxibustion, treatment based on syndrome differentiation obviously exerted an effect on KOA-induced metabolic alterations, which is related to the improvement of oxidative damage, inflammatory, cell autophagy, and energy metabolism.

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Section: Discussionsupporting

confidence: 93%

Metabonomics study on the effects of acupuncture and moxibustion in a rat knee osteoarthritis model

Li,

Tan,

Liang

et al. 2023

Preprint

Self Cite

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“…Furthermore, increased abundance of metabolites in the tryptophan metabolic pathway, such as tryptophan, kynurenic acid, and anthranilic acid, have been found in the synovial fluid of patients with rheumatoid arthritis (42). Additionally, increased tryptophan metabolism has been found in the serum of rats with OA and has been suggested as a biomarker for OA (43). In the current study, we also used Seahorse respirometry, and for the first time, discovered that GH promoted long‐chain FAO in chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Promotion of Joint Degeneration and Chondrocyte Metabolic Dysfunction by Excessive Growth Hormone in Mice

Zhu

Liu

Davis

et al. 2023

Arthritis & Rheumatology

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Objective. Many patients with acromegaly, a hormonal disorder with excessive growth hormone (GH) production, report pain in joints. We undertook this study to characterize the joint pathology of mice with overexpression of bovine GH (bGH) or a GH receptor antagonist (GHa) and to investigate the effect of GH on regulation of chondrocyte cellular metabolism.Methods. Knee joints from mice overexpressing bGH or GHa and wild-type (WT) control mice were examined using histology and micro-computed tomography for osteoarthritic (OA) pathologies. Additionally, cartilage from bGH mice was used for metabolomics analysis. Mouse primary chondrocytes from bGH and WT mice, with or without pegvisomant treatment, were used for quantitative polymerase chain reaction and Seahorse respirometry analyses.Results. Both male and female bGH mice at 13 months of age had increased knee joint degeneration, which was characterized by loss of cartilage structure, expansion of hypertrophic chondrocytes, synovitis, and subchondral plate thinning. The joint pathologies were also demonstrated by significantly higher Osteoarthritis Research Society International and Mankin scores in bGH mice compared to WT control mice. Metabolomics analysis revealed changes in a wide range of metabolic pathways in bGH mice, including beta-alanine metabolism, tryptophan metabolism, lysine degradation, and ascorbate and aldarate metabolism. Also, bGH chondrocytes up-regulated fatty acid oxidation and increased expression of Col10a. Joints of GHa mice were remarkably protected from developing age-associated joint degeneration, with smooth articular joint surface.Conclusion. This study showed that an excessive amount of GH promotes joint degeneration in mice, which was associated with chondrocyte metabolic dysfunction and hypertrophic changes, whereas antagonizing GH action through a GHa protects mice from OA development.

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“…Moreover, NO also alters the function of mitochondria in chondrocytes in OA, which leads to a decrease in cell survival by suppressing the activity of the mitochondrial respiratory chain and ATP synthesis [ 131 ]. The concentration of NO is significantly elevated in the synovial fluid in a model of OA in dogs [ 132 ] and humans [ 133 ]. However, there is evidence that NO has a beneficial effect on some cell types, including osteoblasts.…”

Section: Molecular Mechanisms Of Gasotransmitter-dependent Apoptosis ...mentioning

confidence: 99%

The Role of Gasotransmitter-Dependent Signaling Mechanisms in Apoptotic Cell Death in Cardiovascular, Rheumatic, Kidney, and Neurodegenerative Diseases and Mental Disorders

Rodkin

Nwosu

Sannikov

et al. 2023

IJMS

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Cardiovascular, rheumatic, kidney, and neurodegenerative diseases and mental disorders are a common cause of deterioration in the quality of life up to severe disability and death worldwide. Many pathological conditions, including this group of diseases, are based on increased cell death through apoptosis. It is known that this process is associated with signaling pathways controlled by a group of gaseous signaling molecules called gasotransmitters. They are unique messengers that can control the process of apoptosis at different stages of its implementation. However, their role in the regulation of apoptotic signaling in these pathological conditions is often controversial and not completely clear. This review analyzes the role of nitric oxide (NO), carbon monoxide (CO), hydrogen sulfide (H2S), and sulfur dioxide (SO2) in apoptotic cell death in cardiovascular, rheumatic, kidney, and neurodegenerative diseases. The signaling processes involved in apoptosis in schizophrenia, bipolar, depressive, and anxiety disorders are also considered. The role of gasotransmitters in apoptosis in these diseases is largely determined by cell specificity and concentration. NO has the greatest dualism; scales are more prone to apoptosis. At the same time, CO, H2S, and SO2 are more involved in cytoprotective processes.

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Analysis of Serum Metabolomics in Rats with Osteoarthritis by Mass Spectrometry

Cited by 9 publications

References 40 publications

Metabonomics study on the effects of acupuncture and moxibustion in a rat knee osteoarthritis model

Metabonomics study on the effects of acupuncture and moxibustion in a rat knee osteoarthritis model

Promotion of Joint Degeneration and Chondrocyte Metabolic Dysfunction by Excessive Growth Hormone in Mice

The Role of Gasotransmitter-Dependent Signaling Mechanisms in Apoptotic Cell Death in Cardiovascular, Rheumatic, Kidney, and Neurodegenerative Diseases and Mental Disorders

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