Analysis of Secretion and Expression of Platelet‐derived Growth Factor in Cultured Endothelial Cells From Stroke‐prone Spontaneously Hypertensive Rat

Iihara, Koji; Sasahara, Masakiyo; Saeki, Yukikazu; Hashimoto, Nobuo; Kikuchi, Hiroe; Hazama, Fumitada

doi:10.1111/j.1440-1681.1993.tb01734.x

Cited by 4 publications

(1 citation statement)

References 21 publications

(18 reference statements)

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“…The estimated half-lives based on these plots were = 3, in SHRSP and in WKY, respectively, in quiescent cells. There Total cellular RNA was isolated from the cultured AEC from SHRSP and W K Y following the method of Chomczynski and Sacchi (1987), and northern blot was performed as shown previously (Iihara 1993).…”

Section: Northern Blot Analysismentioning

confidence: 99%

Platelet‐derived Growth Factor B‐chain Comprises the Major Part of Enhanced Released Mitogen From Aortic Endothelial Cells of Stroke‐prone Spontaneously Hypertensive Rats

Sasahara

Hayase

Yang

et al. 1995

Clin Exp Pharma Physio

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1. The present study was conducted to analyse the release and production of mitogen in cultured aortic endothelial cells of stroke-prone spontaneously hypertensive rats (SHRSP), for the further understanding of the role of arterial endothelial cells in the genesis of vascular lesions in hypertension.2. The cultured aortic endothelial cells derived from SHRSP increased released mitogens were compared with those from control Wistar-Kyoto rats (WKY) with respect to cultured vascular medial smooth muscle cells and fibroblasts.3. Biochemical analyses determined that the major part of mitogen released from aortic endothelial cells of both SHRSP and controls was the platelet-derived growth factor B-chain. 4.Further northern analyses revealed that the transcripts of PDGF B-chain were constitutively accumulated three-to fourfold in quiescent aortic endothelial cells from SHRSP, compared with those from WKY through passages 2 to 5. 5. However, the half-lives of the transcripts after actinomycin D treatment were 1.12h (s.d. = 0.14, n = 4) and 1.28h (s.d. = 0.08, n = 3), in SHRSP and in WKY, respectively, showing no significant difference. 6. These suggest that the increased accumulated transcripts of PDGF B-chain in SHRSP are due to an enhanced transcriptional rate. These enhanced release and production of PDGF-B chain in arterial endothelial cells, which may be induced under chronic hypertensive conditions, is suggested to contribute to the genesis of vascular lesion in hypertension, through the stimulation of vascular smooth muscle cell proliferation and hypertrophy.

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Section: Northern Blot Analysismentioning

confidence: 99%

Platelet‐derived Growth Factor B‐chain Comprises the Major Part of Enhanced Released Mitogen From Aortic Endothelial Cells of Stroke‐prone Spontaneously Hypertensive Rats

Sasahara

Hayase

Yang

et al. 1995

Clin Exp Pharma Physio

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Expression of platelet-derived growth factor B-chain in the mature rat brain and pituitary gland

Sasahara

Sato

Iihara

et al. 1995

Molecular Brain Research

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Increased plasma levels of platelet-derived growth factor (PDGF-BB + PDGF-AB) in patients with never-treated mild essential hypertension

Rossi

Casali

Regolisti

et al. 1998

American Journal of Hypertension

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Platelet-derived growth factor (PDGF) could play a role in both vascular hypertrophy and atherosclerotic disease associated with hypertension. To assess whether plasma PDGF level is increased in mild essential hypertension, we measured plasma PDGF concentration in 25 never-treated patients with uncomplicated mild essential hypertension and in 22 normotensive healthy subjects. To evaluate the contribution of platelets to plasma PDGF in the two groups, we also measured plasma beta-thromboglobulin (BTG). Measurement of PDGF was carried out through an enzyme-linked immunoadsorbent assay, which detects two PDGF dimers, namely PDGF-BB and PDGF-AB. Both plasma PDGF and BTG were higher in the hypertensive than in the normotensive subjects. The ratio of PDGF to BTG was similar in the two groups. Plasma PDGF was weakly correlated with plasma BTG in the normotensive subjects, whereas this relationship was lost in the hypertensive patients. Our results suggest that the increase in plasma PDGF (PDGF-AB + PDGF-BB) in never-treated essential hypertension is mainly due to platelet activation. The increased circulating level of PDGF could play a role in the vascular structural changes associated with hypertension.

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Analysis of Secretion and Expression of Platelet‐derived Growth Factor in Cultured Endothelial Cells From Stroke‐prone Spontaneously Hypertensive Rat

Cited by 4 publications

References 21 publications

Platelet‐derived Growth Factor B‐chain Comprises the Major Part of Enhanced Released Mitogen From Aortic Endothelial Cells of Stroke‐prone Spontaneously Hypertensive Rats

Platelet‐derived Growth Factor B‐chain Comprises the Major Part of Enhanced Released Mitogen From Aortic Endothelial Cells of Stroke‐prone Spontaneously Hypertensive Rats

Expression of platelet-derived growth factor B-chain in the mature rat brain and pituitary gland

Increased plasma levels of platelet-derived growth factor (PDGF-BB + PDGF-AB) in patients with never-treated mild essential hypertension

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