Analysis of circular RNA expression profile of pathological bone formation in ankylosing spondylitis

Abstract

Objective: To screen and analyze the function of specific CircRNAs involved in pathological bone formation in patients with ankylosing spondylitis (AS).Methods: From September 2019 to October 2020, hip capsule tissues obtained from 3 patients with AS developed hip joint fusion and 3 patients with femoral neck fracture (FNF) were obtained. The circular RNA expressions of hip capsule were analyzed by Arraystar CircRNA chip. qRT-PCR analysis wan performed to identify the expression patterns of differentially expression CircRNAs.Results: Our findings showed that there were 25 upregulated and 39 down-regulated differential CircRNAs.Among these CircRNAs, we screened 10 highest up-regulatedCircRNAs and 13 lowest down-regulated CircRNAs (Fold Change≥2,P<0.05). In further verification analysis, hsa_circ_0067103, hsa_circ_0004496, and hsa_circ_0002649, ACTG1 were significantly upregulated, while hsa_circ_0020273, hsa_circ_0005699, and hsa_circ_0048764 were markly downregulated in AS tissue than FNF controls.