Analysis of Ruminant Respiratory Syncytial Virus Isolates by RNAse Protection of the G Glycoprotein Transcripts

Alansari, Huda; Duncan, Robert B.; Baker, John C.; Potgieter, L. N. D.

doi:10.1177/104063879901100302

Cited by 10 publications

(8 citation statements)

References 32 publications

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“…The cell types infected by BRSV-GFP were considered to be the same as in the bovine system and therefore, cross-infection of goats may also be an important issue for BRSV. Nucleotide sequence analysis of the G-protein, the most variable among the RSV surface glycoproteins, revealed strong relatedness between bovine and caprine virus isolates as compared to human RSV (HRSV) and ovine RSV (ORSV) isolates (Duncan and Potgieter, 1993;Alansari et al, 1999). Also other reports provided evidence for cross-infection of non-bovine species by BRSV.…”

Section: Discussionmentioning

confidence: 95%

Infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex

Kirchhoff

Uhlenbruck

Keil

et al. 2014

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 95%

Infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex

Kirchhoff

Uhlenbruck

Keil

et al. 2014

Veterinary Microbiology

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“…3,19 Results of an RNase protection assay also support the conclusion that 2 ruminant RSV subgroups exist, 1 representing BRSV and 1 representing ORSV isolates. 2 The bovine and caprine isolates tested were closely related based on G-glycoprotein nucleotide sequences suggesting that bovine and caprine RSV may belong to the same unique subgroup and distinct from the subgroup represented by an ovine RSV. 11 Ruminant RSV isolates include BRSV, ORSV, and caprine RSV.…”

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confidence: 91%

Prevalence of Ovine and Bovine Respiratory Syncytial Virus Infections in Cattle Determined with a Synthetic Peptide-Based Immunoassay

2001

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Abstract. Subgroup-specific peptide-based enzyme-linked immunosorbent assays from the G-protein of the ovine and bovine respiratory syncytial virus (RSV), respectively, were used to determine the prevalence of the ovine and bovine subgroup strains of RSV infections in cattle. A total of 1,102 bovine serum samples were obtained from 6 diagnostic laboratories located in the northwestern and the southeastern USA and were tested for antibody to either the bovine or ovine subgroups of RSV. Antibody to viruses from each subgroup was present in samples from each region and all states tested. The Southeast had a higher prevalence of the bovine subgroup strains (69.5%). Then did the Northwest (40.9%). The prevalence of the ovine strain was similar for the two regions (16.7% in the southeast, 14.9% in the northwest). The overall prevalence was 56.6% for the bovine strain and 15.9% for the ovine strain. These results suggest members of the ovine subgroup of RSV circulate in the cattle population but with less frequency than those viruses of the bovine subgroup.Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract disease in human infants and calves. [3][4][5]7,8,11 Human RSV (HRSV) strains have been divided into 2 major subgroups (A and B) based on their reactions with monoclonal antibodies. 1 The Gglycoprotein from an ovine RSV (ORSV) strain has been cloned and sequenced, and G-protein base sequence comparisons with a bovine RSV (BRSV) strain supports the existence of 2 subgroups of an ungulate RSV. 3,19 Results of an RNase protection assay also support the conclusion that 2 ruminant RSV subgroups exist, 1 representing BRSV and 1 representing ORSV isolates. 2 The bovine and caprine isolates tested were closely related based on G-glycoprotein nucleotide sequences suggesting that bovine and caprine RSV may belong to the same unique subgroup and distinct from the subgroup represented by an ovine RSV. 11 Ruminant RSV isolates include BRSV, ORSV, and caprine RSV. These viruses are members of the genus Pneumovirus in the family Paramyxoviridae and are enveloped, single-stranded, nonsegmented, negativesense RNA viruses. 20 The RNA contains 10 genes that are transcribed to 10 unique mRNAs, each of which encodes a unique protein. 4,[10][11][12] The fusion (F) protein is broadly cross-reactive among strains, whereas the attachment protein G has been the most variable among viral strains. 11,20 Considerable progress has been made concerning the nature of interspecies transmission of RSV

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“…This confirms previous studies suggesting that caprine RSV is closely related to bovine RSV isolates. 1 Nucleotide sequencing of amplified fragments of F and G genes of Bighorn sheep isolate WSU 87-6750 (data not shown) indicated very high level of identity with ovine RSV isolate WSU 83-1578, suggesting that the Bighorn RSV may be considered a member of the ovine RSV subgroup. However, unlike ovine RSV isolate WSU 83-1578, the bighorn sheep RSV strain WSU 87-6750 resisted cleavage using EcoO109I after RT-PCR G because of a point mutation in the cleavage site (data not shown), but they had the same cleavage pattern with EcoRI of their respective F gene amplified products.…”

mentioning

confidence: 99%

“…2 It has been proposed that ungulate RSVs in the United States be divided into 2 subgroups, one representing bovine RSV and the other representing ovine RSV. 1,2,11,14 Experimental infection with ovine RSV in calves resulted in mild to moderate clinical respiratory disease. 3 Also, experimental infection of lambs with bovine RSV produced mild clinical signs and some pulmonary lesions.…”

mentioning

confidence: 99%

Comparison of Targeting F and G Protein Genes to Detect Bovine and Ovine Respiratory Syncytial Viruses

et al. 2003

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In this study, 2 reverse transcriptase–polymerase chain reaction (RT-PCR) assays were developed and compared for simultaneous detection of bovine and ovine respiratory syncytial viruses (RSVs). One assay was based on a set of primers, which amplified a 426-bp fragment of either bovine or ovine RSV F gene (RT-PCR F). The F products could be distinguished by EcoRI or BstYI restriction endonuclease cleavage. In the other assay, a set of primers amplified a 542-bp fragment of either ovine or bovine RSV G gene (RT-PCR G). EcoO1091 and RsaI restriction enzymes were used to differentiate between the ovine and bovine PCR-G products. Sequencing of the PCR products confirmed the fidelity of both assays. The 2 assays were evaluated using 18 bovine RSV isolates, 1 ovine RSV, 1 bighorn sheep RSV isolate, 1 caprine RSV isolate, 2 human RSV isolates, and several other viruses associated with bovine respiratory tract disease. RT-PCR G may be more sensitive in detecting viral RNA. Because the target sequence of the F gene is more conserved than that of the G gene, RT-PCR F followed by the appropriate restriction enzyme cleavage may be superior to RT-PCR G to discriminate between the 2 ruminant RSV subgroups. This assay should prove useful for determining the relative contribution of ovine and bovine RSV to the pathogenesis of bovine respiratory tract disease.

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Analysis of Ruminant Respiratory Syncytial Virus Isolates by RNAse Protection of the G Glycoprotein Transcripts

Cited by 10 publications

References 32 publications

Infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex

Infection of differentiated airway epithelial cells from caprine lungs by viruses of the bovine respiratory disease complex

Prevalence of Ovine and Bovine Respiratory Syncytial Virus Infections in Cattle Determined with a Synthetic Peptide-Based Immunoassay

Comparison of Targeting F and G Protein Genes to Detect Bovine and Ovine Respiratory Syncytial Viruses

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