Analysis of residual oxytetracycline in fresh milk using polymer reversed-phase column

Kaale, Eliangiringa; Chambuso, Mhina; Kitwala, John

doi:10.1016/j.foodchem.2007.08.075

Cited by 30 publications

(18 citation statements)

References 12 publications

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“…The detection limits when validating the HPLC method were set from the standard deviation of the blank sample analysis and the slopes of calibration curves and were 5, 5, and 20 μg/l for oxyteracycline, tetracycline, and chlortetracycline, respectively. Kaale et al (2008) developed a rapid reversed phase high performance liquid chromatography method for OTC analysis in raw milk samples. They obtained average recoveries greater than 92% with RSD ranging between 0.8% and 6.6%.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk

Navrátilová¹,

Borkovcová²,

Dračková³

et al. 2009

Czech J. Food Sci.

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Navrátilová P., Borkovcová I., Dračková M., Janštová B., Vorlová L. (2009): Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk. Czech J. Food, Sci., 27: 379-385.The objective of this study was the detection of tetracycline, chlortetracycline and oxytetracycline residues in raw cow's milk. When analysing bulk milk (n = 57) and tanker trailer'a (n = 113) samples, two methods were used simultaneously: a specific rapid test Milk Tetrasensor Kit and high performance liquid chromatography (HPLC) with ultraviolet detection and isocratic elution. For HPLC analysis, Breeze (Waters, USA), a liquid chromatographic system, was used. The samples underwent solid phase extraction before the HPLC analysis. The Nova Pack C8 column (3.9 × 150 mm, 4 μm, Waters) and mobile phase (0.8 ml/min) consisting of acetonitrile, methanol, and 0.05 mol/l of oxalic acid in a 13:13:74 ratio were used. None of the samples analysed with the use of the specific rapid test displayed the presence of tetracycline antibiotics. In all of the samples analysed by means of HPLC, low concentrations of tetracycline antibiotics residues were detected. None of the samples displayed the presence of chlortetracycline. All of the analysed samples displayed residues of tetracycline. Oxytetracycline residues were detected only in 50.6% of analysed samples.

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Section: Resultsmentioning

confidence: 99%

Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk

Navrátilová¹,

Borkovcová²,

Dračková³

et al. 2009

Czech J. Food Sci.

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“…The other one also provides the determination of 4 epioxytetracycline (4-epiOTC) with OTC, in edible animal tissues such as muscle, liver, kidney, and fat, (Fletouris, Papapanagiotou, & Nakos, 2008). And in the literature there is a LC method with UV detection using polymer reversed-phase column for analysis of OTC was developed and applied in the determination of the antibiotic in fresh milk sample without requiring ion pairing reagents (Kaale, Chambuso, & Kitwala, 2008). The method showed good application for analysis of 100 milk samples.…”

Section: Ultraviolet Detectionmentioning

confidence: 99%

Overview on liquid chromatographic analysis of tetracycline residues in food matrices

Önal

2011

Food Chemistry

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“…[3,4] Consequently, the development of new method is of particular significance for selective removal and separation of TCs in animal-derived foods. Many different methods have been described for the determination of TCs in animal tissues, including liquid chromatography, [5][6][7][8][9][10][11] liquid chromatography-mass spectrometry (LC-MS) [12][13][14][15] thin-layer chromatography (TLC), [16] capillary electrophoresis (CE), [17] and enzyme-linked immunosorbent assay (ELISA) method. [18] Although these methods can produce satisfactory results for detecting TCs, these instrumental analytical procedures usually are not fit for highcapacity quick analysis of TCs.…”

Section: Introductionmentioning

confidence: 99%

Determination of Tetracyclines Residues in Egg, Milk, and Milk Powder by Online Coupling of A Precolumn Packed with Molecular Imprinted Hybrid Composite Materials to RP-HPLC-UV

Zhang

Guo

et al. 2014

Journal of Liquid Chromatography & Related Technologies

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A rapid, specific, and sensitive method was developed for determination of tetracyclines (TCs) in egg, milk, and milk powder samples by online molecularly imprinted solid-phase extraction (MISPE) coupled to a reversed-phase high-performance liquid chromatography (HPLC). A molecularly imprinted polymer hybrid composite material (MIP-HCM) was prepared using TC as the template, methacrylic acid as the functional monomer, tetraethoxysilane as inorganic precursor, and methacryloxypropyltrimethoxysilane as the coupling agent. A new technique of online solid-phase extraction (SPE) coupled with HPLC was established. The conditions of online SPE were optimized. Under the optimal conditions, three TCs residues in egg, milk, and milk powder were separated and detected by the proposed method with better clean-up and recoveries. The recoveries of TCs ranged from 85.3% to 98.3%. The limits of detection and quantitation of the proposed method were in the range of 0.76-1.13 mg=kg and 2.53-3.77 mg=kg, respectively. The MIP-HCM was first used as online SPE packing and the hyphenated technique is promising as a general strategy for direct determination of veterinary drug residues in milk and other biological samples.

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Analysis of residual oxytetracycline in fresh milk using polymer reversed-phase column

Cited by 30 publications

References 12 publications

Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk

Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk

Overview on liquid chromatographic analysis of tetracycline residues in food matrices

Determination of Tetracyclines Residues in Egg, Milk, and Milk Powder by Online Coupling of A Precolumn Packed with Molecular Imprinted Hybrid Composite Materials to RP-HPLC-UV

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