Analysis of Relations between NMDA Receptors and GABA Release at Olfactory Bulb Reciprocal Synapses

Chen, Wei R.; Xiong, Wenhui; Shepherd, Gordon M.

doi:10.1016/s0896-6273(00)81065-x

Cited by 215 publications

(211 citation statements)

References 69 publications

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“…influx through NMDA receptors alone can trigger GABA release at dendrodendritic synapses in the olfactory bulb 12,13 . The study by Llano and colleagues indicates that calcium stores can also supply sufficient calcium to drive fast multivesicular release.…”

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confidence: 99%

Maximinis

Xu‐Friedman

Regehr

2000

Nat Neurosci

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mentioning

confidence: 99%

Maximinis

Xu‐Friedman

Regehr

2000

Nat Neurosci

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“…M/T cells of the same glomerulus receive primary inputs from OSNs of the same type, but each M/T cell also extends additional dendrites and gets complex secondary inputs from local interneurons (4,5), top-down projections (6,7), and from other M/T cells (8,9). M/T cells are also connected through gap junctions within glomeruli (9)(10)(11).…”

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confidence: 99%

Odor coding by modules of coherent mitral/tufted cells in the vertebrate olfactory bulb

Chen

Lin

Schild

2009

Proc. Natl. Acad. Sci. U.S.A.

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calcium imaging ͉ mitral cell ͉ synchronous activity T he glomeruli of the olfactory bulb (OB) constitute an important interface for the coding of odor information. Every individual glomerulus collects convergent inputs from olfactory sensory neurons (OSN) expressing the same olfactory receptor (OR) (1), and it delivers output information to higher brain regions via a number of M/T cells connected to it (2). This modular architecture is thought to play a fundamental role in odor coding (3).M/T cells of the same glomerulus receive primary inputs from OSNs of the same type, but each M/T cell also extends additional dendrites and gets complex secondary inputs from local interneurons (4, 5), top-down projections (6, 7), and from other M/T cells (8, 9). M/T cells are also connected through gap junctions within glomeruli (9-11). While the intraglomerular gap junctions could produce a certain degree of synchronization (9,11,12), the synaptic interactions could transform the initial OSN inputs into spatiotemporal patterns of M/T cell output activity (13-15). As the secondary inputs presumably differ from M/T cell to M/T cell, every M/T cell could process its primary and secondary inputs differentially, which would allow for a large information capacity of M/T cell activity patterns.Three major signal sources thus determine the M/T cell activity patterns conveyed to higher brain centers: primary OSN inputs, currents through intraglomerular gap junctions, and complex secondary synaptic inputs of various origins. As all three input sources of M/T cells depend, directly or indirectly, on odor stimulation, the physiological M/T cell activity patterns must be measured while applying odorants to the olfactory epithelium.Here, we use a nose-brain preparation of the Xenopus tadpole to analyze M/T cell activity pattern following natural odor stimulation. Using a method for identifying neurons that are connected to the same glomerulus, we can observe how natural odorants are represented by modules of glomerulus-specific M/T cells. Results Identification of Glomerulus-specific M/T Cells Using [Ca 2؉ ] Imaging.We investigated the neuronal network of the Xenopus tadpole OB using a combination of [Ca 2ϩ ] imaging and the patch clamp technique. Recordings were obtained from a nose-brain preparation that preserves the connections between the olfactory epithelium and the OB (16, 17) (Fig. S1). This allows us to analyze the circuitry and the response of the OB network to natural odor inputs. In the absence of stimulation, neurons in the M/T cell layer show a rich pattern of spontaneous [Ca 2ϩ ] activities ( Fig. 1A and B) that reflect their spontaneous firing patterns (Fig. S2) (18). The correlation of these activities was low in randomly chosen cell pairs (activity correlation index r ϭ 0.0069 Ϯ 0.0003; n ϭ 50325 pairs). However, a complete search in a correlation matrix (Fig. 1C) revealed specific pairs of neurons that showed strongly correlated activities (r Ͼ 0.6, Fig. 1D, E, and G). These neurons were synchronized for almost every ...

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“…Although recent in vitro studies have elucidated the mechanisms underlying transmitter release at dendrodendritic synapses, the functional role of dendrodendritic inhibition in vivo remains unclear (17)(18)(19)(20). In particular, the relationship between the firing rate of a mitral cell and the strength of recurrent inhibition evoked is not known.…”

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confidence: 99%

“…Propagation of APs into M͞T cell dendrites is thought to be required for evoking glutamate release (17)(18)(19)(20) onto granule cell dendrites. Thus, the propagation of APs in mitral cell lateral dendrites could determine the spatial extent of lateral inhibition evoked by M͞T cell firing.…”

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Action potential propagation in mitral cell lateral dendrites is decremental and controls recurrent and lateral inhibition in the mammalian olfactory bulb

Margrie¹

2000

Proceedings of the National Academy of Sciences

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In the mammalian main olfactory bulb (MOB), the release of glutamate from lateral dendrites of mitral cells onto the dendrites of granule cells evokes recurrent and lateral inhibition of mitral cell activity. Whole-cell voltage recordings in the mouse MOB in vivo and in vitro show that recurrent and lateral inhibition together control the number, duration, and onset of odor-evoked action potential (AP) firing in mitral cells. APs in mitral cells propagate into the lateral dendrites and evoke a transient increase in dendritic calcium concentration ([Ca2+]), which is decremental with distance from the soma, and increases with AP number. These results suggest that the extent of AP propagation in lateral dendrites of mitral cells, along with the concomitant dendritic Ca2+ transient, controls the amplitude of lateral and recurrent inhibition and thus is a critical determinant of odor-specific AP patterns in the MOB

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Analysis of Relations between NMDA Receptors and GABA Release at Olfactory Bulb Reciprocal Synapses

Cited by 215 publications

References 69 publications

Maximinis

Maximinis

Odor coding by modules of coherent mitral/tufted cells in the vertebrate olfactory bulb

Action potential propagation in mitral cell lateral dendrites is decremental and controls recurrent and lateral inhibition in the mammalian olfactory bulb

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