Analysis of red blood cell parameters by Talbot-projected fringes

Abstract

Red blood cell (RBC) anomalies are significant symptoms for identification of health disorders and several blood diseases, which involve the modification of the parameters and biophysical characteristics of such cells. The aim of this study is to measure the three-dimensional phase information of healthy RBCs and their parameters, such as cell diameter, thickness, and hemoglobin (Hb) content, using Talbot-projected fringes. The Talbot image of linear grating is projected onto an RBC slide. The deformed grating lines due to the shape and refractive index of RBCs are recorded by a CCD camera through a 20× microscope objective. Hilbert transform is used to extract the phase image from the deformed projected grating lines. Experimentally calculated values of diameter (8.2 μm), thickness (2.7 μm), and Hb content (28.7 pg/cell) are well within the limits available in the literature. The proposed system is robust and user-friendly and performs the imaging of RBCs with high axial and lateral resolution (2.19 μm).

Low-coherence quantitative differential phase-contrast microscopy using Talbot interferometry

“…The other combination of this structure has also been employed for single-shot phase-shifting interferometry [17]. Talbot projection microscopy has also been investigated for studying the red blood cells morphological properties [18]. Also, the Talbot effect has found applications in rough surface parameters determination [19,20].…”

A Calibration Method for System Parameters in Direct Phase Measuring Deflectometry

“…The past several decades have seen revolutionary developments in three-dimensional (3D) optical shape measurement techniques [1,2], which are now widely used across many different fields, including reverse engineering, 3D games, digitization of cultural relics, clothing, biometrics and other biological fields [3][4][5][6][7][8][9]. In the area of structured light techniques, phase-calculation-based fringe pattern projection has made rapid progress so far, because it has the advantages of full-field measurement, non-contact operation, automatic data processing, and fast data acquisition [10][11][12][13].…”