Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y-STRs)

Abstract

Rapidly mutating (RM) Y-STRs were recently identified and can help to differentiate between paternally related males in paternity and forensic casework. Normal Y-STRs are often used in casework due to their paternal inheritance, which can help to resolve kinship cases, and identify male components in male/female mixtures; however, more discriminating profiles are obtained if rapidly mutating Y-STRs are used. Previously two or three multiplex PCRs have been used to amplify 13 RM Y-STRs; here, an assay amplifying these 13 markers in a single multiplex PCR is described. Commercially available male control DNA samples have been genotyped during the validation of this assay, thus providing a tool for calibrating genotyping results. It is expected that the assay will provide a niche tool for genotyping casework samples.

Monogenic Disorders: An Overview.

“…Hence, there are very limited Y-Linked genetic disorders which are too rare as well (Amberger, J. et al, 2015), which mainly cause the fertility problems and are not usually inherited to the offspring but in some of the cases may be inherited but just affects the sons whereas, the daughters are entirely healthy as they does not carry any Y-Chromosome. Hence, it can be easily distinguished (Hadi, S. 2015). Pedigree for the Y-Linked Inheritance Patterns of the Monogenic Disorders is shown in [Fig.…”