Analysis of Quality-Related Parameters in Mature Kernels of Polygalacturonase Inhibiting Protein (PGIP) Transgenic Bread Wheat Infected with Fusarium graminearum

Abstract

Fusarium head blight, caused by the fungus Fusarium graminearum, has a detrimental effect on both productivity and qualitative properties of wheat. To evaluate its impact on wheat flour, we compared its effect on quality-related parameters between a transgenic bread wheat line expressing a bean polygalacturonase inhibiting protein (PGIP) and its control line. We have compared metabolic proteins, the amounts of gluten proteins and their relative ratios, starch content, yield, extent of pathogen contamination, and deoxynivalenol (DON) accumulation. These comparisons showed that Fusarium significantly decreases the amount of starch in infected control plants, but not in infected PGIP plants. The flour of PGIP plants contained also a lower amount of pathogen biomass and DON accumulation. Conversely, both gluten and metabolic proteins were not significantly influenced either by the transgene or by fungal infection. These results indicate that the transgenic PGIP expression reduces the level of infection, without changing significantly the wheat seed proteome and other quality-related parameters.

Improving Yield Components and Desirable Eating Quality of Two Wheat Genotypes Using Si and NanoSi Particles under Heat Stress

“…Notably, the SDS-PAG analysis revealed the occurrence of marked differences between the two investigated genotypes during the two manipulated dates. The two wheat genotypes showed reductions in the glutenin subunits during the late sowing date, which may play a role in determining the quality characteristics of wheat grain flour, as determined by [ 89 ], who postulated the positive relation between glutenin subunits, particularly low-molecular-weight glutenin, and the good quality of bread wheat. The detected variation in electrophoretic bands of glutenin subunits of the investigated genotypes may be due to the differences in their genetic composition [ 90 ].…”

Time‐resolved dissection of the molecular crosstalk driving Fusarium head blight in wheat provides new insights into host susceptibility determinism

“…Although proteomics already yielded informative data in either the wheat or the fungal side (Chetouhi et al, ; Dornez et al, ; Gunnaiah, Kushalappa, Duggavathi, Fox, & Somers, ), no dual proteomics have been successful. The few proteomics dealing with this pathosystem have failed to identify Fg proteins in planta (Chetouhi et al, ; Ding et al, ; Dornez et al, ; Eggert & Pawelzik, ; Masci et al, ; Zhang, Fu, Hiromasa, Pan, & Bai, ) or have identified less than a dozen (Zhou, Eudes, & Laroche, ). The identification of pathogen's proteins is challenging, because the abundances between the host and the pathogen proteins are strongly unbalanced during the interaction.…”