Time‐resolved dissection of the molecular crosstalk driving Fusarium head blight in wheat provides new insights into host susceptibility determinism

Abstract

Fungal plant diseases are controlled by a complex molecular dialogue that involves pathogen effectors able to manipulate plant susceptibility factors at the earliest stages of the interaction. By probing the wheat–Fusarium graminearum pathosystem, we profiled the coregulations of the fungal and plant proteins shaping the molecular responses of a 96‐hr‐long infection's dynamics. Although no symptoms were yet detectable, fungal biomass swiftly increased along with an extremely diverse set of secreted proteins and candidate effectors supposed to target key plant organelles. Some showed to be early accumulated during the interaction or already present in spores, otherwise stored in germinating spores and detectable in an in vitro F. graminearum exudate. Wheat responses were swiftly set up and were evidenced before any visible symptom. Significant wheat protein abundance changes co‐occurred along with the accumulation of putative secreted fungal proteins and predicted effectors. Regulated wheat proteins were closely connected to basal cellular processes occurring during spikelet ontogeny, and particular coregulation patterns were evidenced between chloroplast proteins and fungal proteins harbouring a predicted chloroplast transit peptide. The described plant and fungal coordinated responses provide a resourceful set of data and expand our understanding of the wheat–F. graminearum interaction.

Unbalanced Roles of Fungal Aggressiveness and Host Cultivars in the Establishment of the Fusarium Head Blight in Bread Wheat

“…Many other studies have also identified F. graminearum genes involved in pathogenicity and some appeared to have only a quantitative effect (i.e., aggressiveness-related genes) (Pariaud et al, 2009). These genes encode secreted proteins and effectors that may play roles in the infection course (Krijger et al, 2014;Lu and Edwards, 2015;Chetouhi et al, 2016;Fabre et al, 2019). Furthermore, previous genomics studies have identified more than 600 genes coding for secreted proteins (Brown et al, 2012;King et al, 2015).…”

“…Furthermore, previous genomics studies have identified more than 600 genes coding for secreted proteins (Brown et al, 2012;King et al, 2015). Some have been identified at the proteome level (Lowe et al, 2015;Fabre et al, 2019) suggesting that F. graminearum could synthesize a large number of proteinous effectors.…”

Identification of Candidate Effector Proteins from Fusarium graminearum during Infection of Arabidopsis thaliana Using Proximity-Dependant Biotin Identification (BioID)

“…A summary of these is provided in Table 2. (Yang et al 2012) or secreted proteins present both in planta and in vitro (Fabre et al, 2019). Paper et al (2007) identified F.…”

“…CEPs were compared with previously published literature on F. graminearum secretomes/proteomes (Brown et al, 2012;Fabre et al, 2019;Ji et al, 2013;Lu & Edwards, 2016;Lysenko et al, 2013;Paper et al, 2007;Phalip et al, 2005;Rampitsch et al, 2013;Taylor et al, 2008;Yang et al, 2012;W. Zhou et al, 2006) and transcriptomes (Brown et al, 2017;Lysøe et al, 2011;Pan et al, 2018;Qinhu Wang et al, 2017).…”

“…Six CEPs were not identified in any of the proteome datasets, and no CEPs were present in all twelve proteome datasets (External Files Table S2. The proteome dataset from Fabre et al (2019) and the exoproteome generated by the Subramaniam lab had the largest overlaps with the identified CEPs, at 83% and 88% respectively (External Files Table S2).…”