Analysis of Proinflammatory and Anti-Inflammatory Cytokine Serum Concentrations in Patients With Multiple Sclerosis by Using a Multiplexed Immunoassay

Martins, Thomas B.; Rose, John; Jaskowski, Troy D.; Wilson, Andrew; Husebye, Dee; Seraj, Hanieh S.; Hill, Harry R.

doi:10.1309/ajcp7ubk8ibvmvnr

Cited by 136 publications

(110 citation statements)

References 32 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These data are supportive of the possible involvement of TMEM66 in the pathological events of MS in the patients, as MS is considered to be mainly mediated by immune response (31). The proinflammatory and anti-inflammatory responses were shown to have clear effects over the time in the progression of the disease (12).…”

Section: Discussionsupporting

confidence: 71%

“…A multiplexed immunoassay was used to assess the concentrations of 13 cytokines/inflammatory markers, including IFN-γ; IL-1β, -2, -4, -5, -6, -8, -10, -12 and -13; TNF-α; IL-2 receptor; and soluble CD40 ligand. Significant increases in the patients compared to the control subjects for IFN-γ, IL-2, IL-1β, TNF-α, IL-4, IL-10 and IL-13 were noted (12).…”

Section: Discussionmentioning

confidence: 87%

“…Adhesion molecules, chemokines, cytokines, T-cell and macrophage infiltration are the main immune mediators that are involved in active MS lesions. Additionally, it is considered that the proinflammatory and anti-inflammatory responses have clear effects over the time of MS progression (12).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential upregulation of the hypothetical transmembrane protein 66 (TMEM66) in multiple sclerosis patients with potential inflammatory response

et al. 2014

View full text Add to dashboard Cite

Abstract. The prevalence of multiple sclerosis (MS) in the Gulf region has markedly increased during the last decade, but the mechanisms of the disease have not been investigated. The present study aimed to understand the molecular processes involved in the disease development of the recently emerged MS in this population using microarray technology to investigate differentially-expressed novel genes in MS patients compared to healthy-matched subjects. The expression of the upregulated genes was confirmed by quantitative polymerase chain reaction (qPCR). Furthermore, gene cloning, protein expression and purification were performed followed by testing of the obtained recombinant protein on biological assays, including cell proliferation and cytokine mRNA detection by reverse transcriptase-qPCR. The results showed that out of ~50,000 genes, the hypothetical transmembrane protein-66 gene (TMEM66) exhibited a 3 times higher expression in MS patients compared to healthy subjects. The TMEM66 gene was cloned and its protein showed marked immunological activity relevant to MS since significant proliferation (P<0.05) and augmented induction of the proinflammatory cytokines, interleukin (IL)-6, interferon-γ, tumor necrosis factor-α, and the chemokines, chemokine ligand 5/chemokine receptor 5, macrophage inflammatory protein 1α (MIP-1α) and MIP-1β were recorded, but not the anti-inflammatory cytokines, IL-4 or IL-2. In conclusion, TMEM66 may be associated with the molecular events of MS and may be considered as an MS biomarker for future personalized medicine management approaches.

show abstract

Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Differential upregulation of the hypothetical transmembrane protein 66 (TMEM66) in multiple sclerosis patients with potential inflammatory response

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Such a disruption might play a role in the increased incidence of various medical conditions in shift workers, such as the increased risk for infections, autoimmune diseases, cancer, and cardiovascular and metabolic disorders (2,(8)(9)(10)(11)(12)(13). Interestingly, an increased prevalence of autoimmune conditions, such as multiple sclerosis and autoimmune thyroid disorders, was associated with shift work (11,12,(60)(61)(62)(63). In autoimmune thyroid disorders, there is an increase in the recruitment of Th1 lymphocytes in thyroid tissue (based on changes in cell trafficking between blood and tissues), leading to an increase in the production of IFN-g and TNF-a, further stimulating the recruitment of Th1 lymphocytes and initiating an amplification feedback loop (63).…”

Section: Discussionmentioning

confidence: 99%

Simulated Night Shift Disrupts Circadian Rhythms of Immune Functions in Humans

Cuesta

Boudreau

Dubeau-Laramée

et al. 2016

The Journal of Immunology

107

View full text Add to dashboard Cite

Recent research unveiled a circadian regulation of the immune system in rodents, yet little is known about rhythms of immune functions in humans and how they are affected by circadian disruption. In this study, we assessed rhythms of cytokine secretion by immune cells and tested their response to simulated night shifts. PBMCs were collected from nine participants kept in constant posture over 24 h under a day-oriented schedule (baseline) and after 3 d under a night-oriented schedule. Monocytes and T lymphocytes were stimulated with LPS and PHA, respectively. At baseline, a bimodal rhythmic secretion was detected for IL-1β, IL-6, and TNF-α: a night peak was primarily due to a higher responsiveness of monocytes, and a day peak was partly due to a higher proportion of monocytes. A rhythmic release was also observed for IL-2 and IFN-γ, with a nighttime peak due to a higher cell count and responsiveness of T lymphocytes. Following night shifts, with the exception of IL-2, cytokine secretion was still rhythmic but with peak levels phase advanced by 4.5–6 h, whereas the rhythm in monocyte and T lymphocyte numbers was not shifted. This suggests distinct mechanisms of regulation between responsiveness to stimuli and cell numbers of the human immune system. Under a night-oriented schedule, only cytokine release was partly shifted in response to the change in the sleep–wake cycle. This led to a desynchronization of rhythmic immune parameters, which might contribute to the increased risk for infection, autoimmune diseases, cardiovascular and metabolic disorders, and cancer reported in shift workers.

show abstract

“…Experimental autoimmune encephalomyelitis (EAE) is an acceptable model for the study of MS which is induced by immunization with myelin antigens or by adoptive transfer of myelin-specifi c T cells (2). Investigating changes in proinfl ammatory and anti-infl ammatory cytokines over time of disease progression have led to an insight into the pathogenesis of MS (3).…”

Section: Introductionmentioning

confidence: 99%

Astaxanthin effectiveness in preventing multiple sclerosis in animal model

Bidaran¹,

Ahmadi²,

Yaghmaei³

et al. 2018

BLL

View full text Add to dashboard Cite

OBJECTIVE: The aim of the present study was to reveal the effect of therapeutic and prophylactic potential of astaxanthin in experimental autoimmune encephalomyelitis (EAE) as an acceptable model for the study of multiple sclerosis (MS). BACKGROUND: Astaxanthin has powerful antioxidant activities as well as several essential biological functions while multiple sclerosis prevention is highly regarded by researchers. METHODS: The astaxanthin potential in prevention of multiple sclerosis was examined in the chronic model of experimental autoimmune encephalomyelitis (EAE) by using female C57BL/6 mice induced with oligodendrocyte glycoprotein (MOG). Splenocytes were assessed to measure the levels of proinfl ammatory and antiinfl ammatory cytokines, proliferation rate and FoxP3+Treg cell frequency. Immunohistochemical examinations were performed on spinal cord and brain tissue. RESULTS: Astaxanthin reduced splenocytes proliferation index and proinfl ammatory cytokine levels, and vice versa increased the anti-infl ammatory cytokine levels. Immunohistochemical studies of the spinal cord and brain showed that the infi ltration with infl ammatory cells was highly confi ned in the central nervous system. Protective effects of astaxanthin were visible by assigning low score recording in clinical behavior and disease severity. CONCLUSION: Astaxanthin is a powerful tool for intervention in EAE on a model of multiple sclerosis, so it can be studied further to prevent and treat MS (Tab. 2, Fig. 3, Ref. 41). Text in PDF www.elis.sk.

show abstract

Analysis of Proinflammatory and Anti-Inflammatory Cytokine Serum Concentrations in Patients With Multiple Sclerosis by Using a Multiplexed Immunoassay

Cited by 136 publications

References 32 publications

Differential upregulation of the hypothetical transmembrane protein 66 (TMEM66) in multiple sclerosis patients with potential inflammatory response

Differential upregulation of the hypothetical transmembrane protein 66 (TMEM66) in multiple sclerosis patients with potential inflammatory response

Simulated Night Shift Disrupts Circadian Rhythms of Immune Functions in Humans

Astaxanthin effectiveness in preventing multiple sclerosis in animal model

Contact Info

Product

Resources

About