Analysis of prevalence and CRISPR typing reveals persistent antimicrobial-resistant Salmonella infection across chicken breeder farm production stages

Fei, Xiao; He, Xiaohong; Guo, Rong; Yin, Chao; Geng, Hui; Wu, Kaiyue; Yin, Kequan; Geng, Shizhong; Pan, Zhiming; Li, Qiuchun; Jiao, Xinan

doi:10.1016/j.foodcont.2017.01.023

Cited by 18 publications

(10 citation statements)

References 51 publications

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“…Of 37 S. Enteritidis isolates, 35 contained the point mutant in gyrA gene for nalidixic acid resistance. A previous study showed that S. Enteritidis were highly resistant to nalidixic acid (91.3%), ampicillin (39.13%), and streptomycin (28.70%) in Jiangsu province, China (42), which were confirmed with our antimicrobial genotype analysis. Moreover, a study from Thailand also demonstrated similar results, in which S. Enteritidis showed highest resistance rates to nalidixic acid (83.2%) and ampicillin (50.05%) (43).…”

Section: Discussionsupporting

confidence: 87%

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

Wang

Tian

et al. 2020

Front. Vet. Sci.

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Section: Discussionsupporting

confidence: 87%

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

Wang

Tian

et al. 2020

Front. Vet. Sci.

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“…Based on our results, the detection rate of Salmonella -positive eggs in the production (6.6%) and sales (5.1%) links were similar. A previous investigation provided a detailed description of contamination during five breeder farm production stages (i.e., laying, hatching, rearing, brooding, and post-hatching), with a considerably high prevalence of egg samples containing Salmonella found at the laying (29.2%) and hatching (21.6%) stages ( Fei et al, 2017 ). In the current study, a total of 304 eggs were collected from three different poultry farms of different scales in Yangling.…”

Section: Discussionmentioning

confidence: 99%

Prevalence, Serotype, Antibiotic Susceptibility, and Genotype of Salmonella in Eggs From Poultry Farms and Marketplaces in Yangling, Shaanxi Province, China

Zheng

et al. 2020

Front. Microbiol.

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Poultry products such as eggs provide essential nutrients to the human body and thus play vital roles in the human food network. Salmonella is one of the most notorious foodborne pathogens and has been found to be prevalent in eggs. To better understand the characteristics of Salmonella in eggs, we investigated the prevalence of Salmonella spp. in 814 fresh eggs collected from poultry farms and retail marketplaces in Yangling, Shaanxi Province, China. The serotype, genotype, and antibiotic susceptibilities of 61 Salmonella isolates recovered from the eggs were analyzed. The average detection rate of Salmonella -positive eggs was 5.6%, with 6.6% of the eggs collected from poultry farms and 5.1% from marketplaces. Thirteen serotypes were identified from the 61 isolates, among which Salmonella Typhimurium (24.5%) and Salmonella Indiana (22.9%) were the most prevalent serotypes. Other dominant serotypes included Salmonella Thompson (13.1%) and Salmonella Enteritidis (11.4%), with the remaining nine serotypes detected at low rates (1.6–4.9%). All the Salmonella isolates tested were resistant to sulfisoxazole (100.0%). The majority (77.1%) of the isolates were resistant to nalidixic acid, amoxicillin-clavulanate, and ampicillin, while nearly two-thirds (63.9–68.9%) were resistant to trimethoprim–sulfamethoxazole, kanamycin, tetracyclines, and chloramphenicol. The rate of resistance to ciprofloxacin was 40.1%; the resistance rates to streptomycin, ceftiofur, and ceftriaxone ranged from 21.3 to 26.2%; and those to gentamicin, amikacin, and cefoxitin were relatively low (3.3–16.4%). Forty-nine (80.3%) Salmonella isolates exhibited resistance to multiple antibiotics, 20 (32.8%) of which were resistant to at least 10 antibiotics. Subtyping by pulse-field gel electrophoresis revealed a close genetic relatedness of Salmonella isolates from poultry farms, in striking contrast to the high diversity of the isolates obtained from marketplaces. Isolates of the same serotype always shared identical genotype and antibiotic resistance profiles, even the ones that were recovered from eggs sampled at different locations and times. These findings indicate that diverse Salmonella spp. with high rates of multidrug resistance are prevalent in fresh eggs in the study area. More attention should be paid to egg production, transportation, and storage to prevent foodborne outbreaks caused by Salmonella .

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“…In the present study, the prevalence of S. enterica (16.4%) was higher in hatcheries than in its upstream breeder farm (3.0%), despite fumigation being routinely used during hatching ( Table S2 ) [ 16 ]. The prevalence of Salmonella in hatcheries varied widely from operation to operation (6.78–44.9%) and may have been associated with differences in hygiene and sanitation levels of each operation and the different detection methods used in each study [ 25 , 36 , 37 ]. Herein, the Salmonella isolation rate from breeder farms was relatively lower (3.0%) than in previous studies in Korea (14.7–19.0%) and China (10.53–18.15%); however, even infected breeder flocks have been shown to cause widespread Salmonella contamination [ 18 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

The Occurrence of Antimicrobial-Resistant Salmonella enterica in Hatcheries and Dissemination in an Integrated Broiler Chicken Operation in Korea

Shang

Wei

Cha

et al. 2021

Animals

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Positive identification rates of Salmonella enterica in hatcheries and upstream breeder farms were 16.4% (36/220) and 3.0% (6/200), respectively. Among the Salmonella serovars identified in the hatcheries, S. enterica ser. Albany (17/36, 47.2%) was the most prevalent, followed by the serovars S. enterica ser. Montevideo (11/36, 30.6%) and S. enterica ser. Senftenberg (5/36, 13.9%), which were also predominant. Thirty-six isolates showed resistance to at least one antimicrobial tested, of which 52.8% (n = 19) were multidrug resistant (MDR). Thirty-three isolates (enrofloxacin, MIC ≥ 0.25) showed point mutations in the gyrA and parC genes. One isolate, S. enterica ser. Virchow, carrying the blaCTX-M-15 gene from the breeder farm was ceftiofur resistant. Pulsed-field gel electrophoresis (PFGE) showed that 52.0% S. enterica ser. Montevideo and 29.6% S. enterica ser. Albany isolates sourced from the downstream of hatcheries along the broiler chicken supply chain carried the same PFGE types as those of the hatcheries. Thus, the hatcheries showed a high prevalence of Salmonella isolates with high antimicrobial resistance and no susceptible isolate. The AMR isolates from hatcheries originating from breeder farms could disseminate to the final retail market along the broiler chicken supply chain. The emergence of AMR Salmonella in hatcheries may be due to the horizontal spread of resistant isolates. Therefore, Salmonella control in hatcheries, particularly its horizontal transmission, is important.

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Analysis of prevalence and CRISPR typing reveals persistent antimicrobial-resistant Salmonella infection across chicken breeder farm production stages

Cited by 18 publications

References 51 publications

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

Prevalence of Salmonella Isolates and Their Distribution Based on Whole-Genome Sequence in a Chicken Slaughterhouse in Jiangsu, China

Prevalence, Serotype, Antibiotic Susceptibility, and Genotype of Salmonella in Eggs From Poultry Farms and Marketplaces in Yangling, Shaanxi Province, China

The Occurrence of Antimicrobial-Resistant Salmonella enterica in Hatcheries and Dissemination in an Integrated Broiler Chicken Operation in Korea

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