Analysis of predicted amino acid biosynthesis in Rv3344c in Mycobacterium tuberculosis H₃₇Rv using bioinformatics tools

Abstract

According to World Health Organization (WHO) report, Mycobacterium tuberculosis H37Rv (M. tuberculosis) affects one‐third population of the world. Emergence of effective treatment/research against this disease is need of the hour. Therefore, we present some important aspects of Rv3344c, which is a PE_PGRS protein. Evidence shows that PE_PGRS proteins show fibronectin binding activity. This protein has affinity for calcium and also shows motifs of GTP‐binding protein. It also shows the presence of sites for ribose‐5‐phosphate binding and motifs of aspartate‐beta‐semialdehyde dehydrogenase, both of which are involved in amino acid biosynthesis. Thus, this protein might be targeted to block the amino acid biosynthesis in M. tuberculosis. This article takes into consideration some important aspects of Rv3344c protein as its function is still unknown. This study includes retrieval of protein sequence database, multiple sequence alignment, protein–protein interaction, epitope prediction, localization, function prediction, phosphorylation site prediction, model building and its validation, ligand‐binding prediction along with mutational analysis. Hence, this study might be an important step in the development of new drugs and treatment of tuberculosis.

Design of a Multi-Epitope Vaccine against Tuberculosis from Mycobacterium tuberculosis PE_PGRS49 and PE_PGRS56 Proteins by Reverse Vaccinology

“…The first, an in-silico study on PE_PGRS49, predicted MHC-II binding sites as well as the presence of GTP-binding motifs, which might serve as a recognition target for human immune cells. PE_PGRS49 also exhibits motifs of an enzyme involved in amino acid biosynthesis and ligand-binding sites that are involved in amino acid and nucleotide biosynthesis, characteristics that suggest the participation of PE_PGRS49 in amino acid biosynthesis [ 55 ]. The second, a systematic study of the genetic determinants of TB transmission at the level of individual strains, was performed by molecular and conventional epidemiology with in vitro immunological assays to identify bacterial factors associated with tuberculosis transmission.…”