Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle

Yan, Lin; Fang, Xinggen; Zhang, R.F.; Zhang, C.L.; Chen, H.

doi:10.1080/09712119.2011.607920

Cited by 10 publications

(5 citation statements)

References 10 publications

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“…Zhou et al (2016) found an association between the POU1F1/AluI locus and the LMY, while other authors have reported associations between different parts of the POU1F1 gene and the LMY (Özmen et al, 2014;Lan et al, 2013;Mura et al, 2012) and milk production traits in cattle (Khaizaran and Al-Razem, 2014). However, in the study conducted by Huang et al (2008), a significant effect of the POU1F1 gene on the LMY was observed only for exon 3, whereas no effect was observed for intron 5 nor exon 6 (Trakovicka et al, 2014;Yan et al, 2011). In our study, the TC genotype of POU1F1 was associated with a longer lactation length compared with the CC and TT genotypes.…”

Section: Discussioncontrasting

confidence: 59%

Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats

Gündüz,

Biçer

2023

Arch. Anim. Breed.

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Abstract. This study aimed to investigate the impact of single-nucleotide polymorphisms (SNPs) on milk production traits in Kilis dairy goats by analyzing the genotypes of POU1F1, PRLR, β-Lg, GH1, and GH2 genes and their association with lactation milk yield (LMY), lactation length (LL) and average daily milk yield (ADMY). Blood samples were collected from 227 goats, and genotyping was performed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results revealed that the frequencies of the genotypes varied among the genes. The polymorphisms were found to be significantly linked with milk production traits. These findings suggest that SNPs of POU1F1/AluI, PRLR/RsaI and β-Lg/SacII are significantly associated with LMY and that the POU1F1-TC genotype, PRLR-TT genotype and β-Lg-AB genotype are associated with higher LMY and ADMY. Additionally, the POU1F1-TC genotype was found to have a longer LL. However, no significant association was found between the GH1 and GH2 genotypes and LMY, LL and ADMY. Overall, this study provides valuable insights into the genetic factors influencing milk production traits in Kilis dairy goats, which can be utilized for the selection of high-yielding animals in breeding programs.

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Section: Discussioncontrasting

confidence: 59%

Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats

Gündüz,

Biçer

2023

Arch. Anim. Breed.

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“…Previous studies evaluating Pit-1-HinfI polymorphism in Holsteins bred in Iran (9,12), Poland (10), Italy (36) and China (43) found that the frequency of the Pit-1-B allele was much higher than that of the A allele frequency at 0.830 and 0.70, respectively. In the present study of Holstein cattle, BB was genotype found at the highest frequency with a value between 0.569 and 0.742 and the AA genotype was present at the lowest frequency, between 0.007 and 0.054.…”

Section: Discussionmentioning

confidence: 90%

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Bayram¹,

Arslan²,

Akyüz³

et al. 2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X 2 :40.75, p<0.001and X 2 :8.4, p<0.01). Türkiye'de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü-1 (PIT-1) ve leptin (LEP) gen polimorfizmlerinin belirlenmesiÖzet: Bu çalışmada Türkiye'de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç'lik ve LEP geni için 422 bç'lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X 2 :40.75, p<0.001ve X 2 :8.4, p<0.01).

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“…Further, it was significantly linked with milk production in goat and sheep (Lan et al, 2009 c ;Ozmen et al, 2014); with litter size in Awassi sheep and Shaanbei W hite Cashmere goat (Al-Khuzai and Al-Anbari 2018; Zhu et al, 2019). The POU1F1 gene mutations also exhibited a positive association with milk protein in cattle (Yan et al, 2011); growth and carcass traits of swine (Getmantseva et al, 2017).…”

Section: Association Of Exon-6 Pou1f1 Gene Polymorphism With Body Wei...mentioning

confidence: 99%

POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat

Pawar

Sawane

Doiphode

2021

IJAR

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Background: Osmanabadi is the premium meat type goat breed of Maharashtra, known for its adaptability and reproductive efficiency. Identification of superior germplasm and incorporating them into breeding programme is the need of hour for augmenting productivity of Osmanabadi as well as other indigenous goat breeds. Hence, the present research was aimed to study Pituitary Specific Transcription Factor I (POU1F1) gene polymorphism and its associations with body weight of Osmanabadi goat. Methods: Genomic DNA was extracted from 217 blood samples of randomly selected Osmanabadi goats. The 450 bp fragment of POU1F1 gene encompassing exon-6 and its flanking region (3’UTR) was PCR amplified. The PCR amplicon was subjected to RFLP using AluI and PstI restriction enzymes to identify polymorphism at nucleotide position 174 (T greater than C) in exon 6 and at nucleotide position 110 (T greater than C) in the 3’UTR of POU1F1 gene, respectively. The association of the observed allelic variants / genotypes with body weight of the animals was performed using One-way ANOVA. Result: The AluI PCR-RFLP of 450 bp amplicon of POU1F1 gene in Osmanabadi goats revealed polymorphism with three different genotypes viz., ‘CC’, ‘TT’ and ‘TC’. The observed frequencies for TT, TC and CC genotypes were 0.604, 0.355 and 0.041 respectively. The frequencies of T and C alleles were found to be 0.781 and 0.219, respectively. However, PstI PCR-RFLP revealed monomorphic 3’UTR of POU1F1 gene with single TT genotype. We observed significant differences (P less than 0.01) between various genotypes at AluI exon-6 locus of POU1F1 gene and body weights at birth, 3 and 6 months of Osmanabadi goat. Highest body weight was recorded for TT genotype followed by TC and CC genotypes in all three age groups. Our findings indicate that the AluI PCR-RFLP locus of the exon-6 of POU1F1 gene can be used as a potent molecular marker for selection of superior stock of Osmanabadi goats.

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Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle

Cited by 10 publications

References 10 publications

Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats

Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat

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Analysis of pituitary specific transcription factor-1 gene polymorphism in several indigenous Chinese cattle and crossbred cattle

Cited by 10 publications

References 10 publications

Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats

Milk-y Way: the impact of single-nucleotide polymorphisms on milk production traits in Kilis dairy goats

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

​POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat

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POU1F1 Gene Polymorphism and its Associations with Body Weight of Osmanabadi Goat