Analysis of Phylogenetic Relationships of Main Citrus Germplasms Based on Ftir Spectra of Petals

Abstract

To develop a quick, accurate and reliable technique for studying phylogenetic relationship of Citrus, FTIR (Fourier transform infrared spectroscopy) technique was used. The petals spectra of eighteen varieties of citrus germplasms were investigated by FTIR. Pretreatment methods of raw spectra (2000-500 cm 21 ) were composed of baseline correction, normalize and first derivative (Savitzky-Golay). We used One-way ANOVA (analysis of variance) and Tukey's HSD (honestly significant difference) to extract effective wave bands, where the spectral absorbance values of different citrus germplasms were significantly different. The results showed that 2000 , 1831 cm 21 , 1763 , 1595 cm 21 , 1517 , 1090 cm 21 , 1035 , 1024 cm 21 , 950 , 935 cm 21 , 861 , 784 cm 21 , 744 , 721 cm 21 and 653 , 608 cm 21 were the effective wave bands. HCA (hierarchical cluster analysis) was adopted to classify citrus germplasms based on the above eight effective wave bands. It was found that eighteen citrus varieties were classified into six subgroups. The results of classification and citrus phylogenetic relationships between six subgroups were consistent of results from Morphology, Biochemistry, Cytology and Molecular Biology. The overall results demonstrated that fourier transform infrared spectroscopy technique with One-way ANOVA and Tukey's HSD and hierarchical cluster analysis model were promising for the rapid, accurate and reliable classification for citrus as well as studying citrus phylogenetic relationship.

Environmentally-related genotypic, phenotypic and metabolic diversity of oat (Avena sativa L.) landraces based on 67 Polish accessions

“…The biochemical characterization of germplasm was performed using infrared spectroscopy, a convenient approach for high-throughput analysis, which provided a metabolic fingerprint of a sample. The feasibility of this method for discriminating and taxonomic applications have been successfully proved in researches with various plant organs or parts including seeds (Demir et al 2015;Liu and Yu 2011), leaves (Kim et al 2004;Lu et al 2008), petals (Li et al 2015), roots (Naumann et al 2010), or pollens (Zimmermann and Kohler 2014).…”