Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells

Bystrov, F G; Makarov, Vladimir; Поминова, Д. В.; Рябова, А. В.; Лощенов, В. Б.

doi:10.24931/2413-9432-2016-5-1-3-8

Cited by 18 publications

(5 citation statements)

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“…Firstly, the surface molecules detached from the surface-thus, some nanoparticles disperse into the molecules. Secondly, it makes some surface molecules turn perpendicular to the surface of the nanoparticle [20] (more details are provided in the discussion section of this article). Additional activation allows the reduction of the time for FD of the tooth enamel.…”

Section: Research Resultsmentioning

confidence: 99%

Fluorescent diagnostics using aluminum phthalocyanine nanoparticles as a detection method for enamel microcracks

Zolotareva¹,

Loschenov²

2018

Laser Phys. Lett.

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Early diagnosis of caries and enamel microcracks is of great importance for the prevention of the destruction of healthy tooth enamel. It was reported that aluminum phthalocyanine in the form of nanoparticles (nAlPc) can be used for fluorescent diagnostics of tooth enamel microcracks. A model compound with the nAlPc colloid and Protelan MST-35 were prepared for the development of the method of laser-induced fluorescent diagnostics (FD). Two groups of human teeth were used to research the interaction between the nAlPc colloid and the model compound for the detection of microcracks and the areas of accumulation of pathogenic microflora on the enamel surface. A Student’s two-sample unequal variances t-test was used to check whether the means of the coefficient of diagnostics contrast (kdc) between two groups of samples was significantly different. Experimental studies were conducted using a laser electronic spectro-analyser and a fluorescence video imaging system. The results of the studies of the nAlPc colloid interaction with the surface enamel of the tooth enamel ex vivo in the first group showed that in 3 min a low-intensity nAlPc fluorescence was observed. This was due to a slight activation of the surface molecules of nAlPc by a pathogenic microflora. Measurements of the interaction of the model compound (nAlPc colloid with the Protelan MST-35 and supplementary components) with the tooth enamel has shown that the high-intensity AlPc molecule fluorescence appears in the enamel microcracks 3 min after application. The high intensity of the fluorescence is accounted for by a preliminary activation of nAlPc by the surfactant as they meet pathogenic microflora. Statistical analysis of the results revealed a statistically significant difference between the kdc of the studied groups (α = 0.05). It has been concluded that the developed method of laser-induced FD of tooth enamel makes it possible to detect the hidden microcracks on the enamel surface and the hidden accumulation areas of the pathogenic microflora using the model compound.

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Section: Research Resultsmentioning

confidence: 99%

Fluorescent diagnostics using aluminum phthalocyanine nanoparticles as a detection method for enamel microcracks

Zolotareva¹,

Loschenov²

2018

Laser Phys. Lett.

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“…The system works on a time-correlated single photon counting approach. The description of the system is reported in the [29]. The fiber was fixed on a tripod to maintain the working distance between the distal end and the tumor tissue.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Fluorescence Decay Kinetics of Indocyanine Green Monomers and Aggregates in Brain Tumor Model In Vivo

et al. 2021

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Spectroscopic approach with fluorescence time resolution allows one to determine the state of a brain tumor and its microenvironment via changes in the fluorescent dye’s fluorescence lifetime. Indocyanine green (ICG) is an acknowledged infra-red fluorescent dye that self-assembles into stable aggregate forms (ICG NPs). ICG NPs aggregates have a tendency to accumulate in the tumor with a maximum accumulation at 24 h after systemic administration, enabling extended intraoperative diagnostic. Fluorescence lifetime analysis of ICG and ICG NPs demonstrates different values for ICG monomers and H-aggregates, indicating promising suitability for fluorescent diagnostics of brain tumors due to their affinity to tumor cells and stability in biological tissue.

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“…For the evaluation of luminescence lifetime of PS the Hamamatsu streak-camera with a time-resolution was used [28]. It comprised a laser source Picosecond Light Pulser PLP-10 (Japan) with fiber optic output generating at the wavelength of 637 nm with a pulse duration of 65 ps and a polychromator with fiber optic input and Semrock optical filter LD 01-785/10-12.5 inlet that transmits only the spectral region of luminescence band of bacteriochlorins and minimized the impact of third-party flashes.…”

Section: Methodsmentioning

confidence: 99%

Photosensitizers for antibacterial photodynamic therapy based on tetracationic derivatives of synthetic bacteriochlorins

et al. 2018

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Antibacterial photodynamic therapy is a promising method of treating local infected foci, in particular, surgical and burn wounds, trophic and diabetic ulcers. Both Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Pseudomonas aeruginosa are found in most infected wounds. Photodynamic inactivation (PDI) is able to effectively destroy bacterial cells without developing resistance in response to treatment. This work is dedicated to the study of photophysical and antibacterial properties of new photosensitizers based on tetracationic synthetic bacteriochlorins with a reduced size of the molecule and molecular weight for PDI of S. aureus and P. aeruginosa bacteria and their biofilms.

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Analysis of photoluminescence decay kinetics of aluminum phthalocyanine nanoparticles interacting with immune cells

Cited by 18 publications

References 0 publications

Fluorescent diagnostics using aluminum phthalocyanine nanoparticles as a detection method for enamel microcracks

Fluorescent diagnostics using aluminum phthalocyanine nanoparticles as a detection method for enamel microcracks

Analysis of Fluorescence Decay Kinetics of Indocyanine Green Monomers and Aggregates in Brain Tumor Model In Vivo

Photosensitizers for antibacterial photodynamic therapy based on tetracationic derivatives of synthetic bacteriochlorins

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