Analysis of phosphorus-deficient responsive miRNAs and cis-elements from soybean (Glycine max L.)

Zeng, Houqing; Zhu, Yiyong; Huang, Shudong; Yang, Zhi Min

doi:10.1016/j.jplph.2010.04.017

Cited by 96 publications

(73 citation statements)

References 56 publications

(71 reference statements)

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“…We found that the putative promoter regions of miR399a, miR399b, and miR399c also contain GNA-TATNC elements (data not shown). Soybean (Glycine max) miRNA genes responsible for Pi starvation signaling contain several types of Pi-responsive ciselements in their promoters, including the PHR1-binding site (Zeng et al, 2010). This suggests that the induction of several miRNA399 family members, including miRNA399f, in response to Pi limitation could be mediated in part by PHR1.…”

Section: Discussionmentioning

confidence: 99%

Regulation of miR399f Transcription by AtMYB2 Affects Phosphate Starvation Responses in Arabidopsis

Baek

Kim²,

Chun³

et al. 2012

Plant Physiology

133

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Although a role for microRNA399 (miR399) in plant responses to phosphate (Pi) starvation has been indicated, the regulatory mechanism underlying miR399 gene expression is not clear. Here, we report that AtMYB2 functions as a direct transcriptional activator for miR399 in Arabidopsis (Arabidopsis thaliana) Pi starvation signaling. Compared with untransformed control plants, transgenic plants constitutively overexpressing AtMYB2 showed increased miR399f expression and tissue Pi contents under high Pi growth and exhibited elevated expression of a subset of Pi starvation-induced genes. Pi starvation-induced root architectural changes were more exaggerated in AtMYB2-overexpressing transgenic plants compared with the wild type. AtMYB2 directly binds to a MYB-binding site in the miR399f promoter in vitro, as well as in vivo, and stimulates miR399f promoter activity in Arabidopsis protoplasts. Transcription of AtMYB2 itself is induced in response to Pi deficiency, and the tissue expression patterns of miR399f and AtMYB2 are similar. Both genes are expressed mainly in vascular tissues of cotyledons and in roots. Our results suggest that AtMYB2 regulates plant responses to Pi starvation by regulating the expression of the miR399 gene.Phosphorus (P) is an essential component of all organisms, as it is found, among other compounds, in nucleic acids, ATP, and membrane phospholipids. It is an essential nutrient for plants. P can be acquired by plants only as inorganic phosphate (Pi). Therefore, most of the P content of soils is unavailable for plant growth and development (Hinsinger, 2001). To overcome the problem of Pi limitation, plants have developed a variety of adaptive responses that conserve internal P while activating mechanisms that enhance the accessibility and uptake of external P. The accompanying gene expression changes produce changes in root architecture, enhanced Pi uptake activity, secretion of organic acids, and secretion of phosphatases (Raghothama, 1999;Poirier and Bucher, 2002;Yuan and Liu, 2008;Péret et al., 2011). The synchronization of Pi availability with plant growth and development is orchestrated by several phytohormones, including abscisic acid, ethylene, auxin, and cytokinin (Hillwig et al., 2008;Devaiah et al., 2009;Lei et al., 2011).A few transcription factors have been characterized that appear to regulate subsets of the response to Pi stress, either positively or negatively. PHOSPHATE STARVATION RESPONSE1 (PHR1) is a MYB transcription factor that initiates the up-regulation of Pi starvation-responsive genes in plants and unicellular algae (Rubio et al., 2001). WRKY75, a WRKY transcription factor family member, has been identified as a key regulator of Pi acquisition and root architecture in response to Pi starvation (Devaiah et al., 2007a). MYB62, an R2R3-type MYB transcription factor, connects Pi homeostasis and GA signaling during Pi starvation (Devaiah et al., 2009). ZAT6, a C2H2-type zinc finger transcription factor, regulates Pi homeostasis and exerts some control over root development (...

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Section: Discussionmentioning

confidence: 99%

Regulation of miR399f Transcription by AtMYB2 Affects Phosphate Starvation Responses in Arabidopsis

Baek

Kim²,

Chun³

et al. 2012

Plant Physiology

133

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“…The expression of miR399 primary transcripts is also positively regulated by the overexpression of the rice PHR1 homolog, OsPHR2 (Zhou et al, 2008). To investigate the transcriptional regulation of PSR miRNAs, a computational analysis of Pi-responsive cis-elements in promoter sequences was conducted in soybean (Glycine max; Zeng et al, 2010). The analysis revealed an overrepresentation of P1BS, PHO, W-box, and NIT2 elements.…”

Section: Regulation Of P Starvation-responsive Mirnasmentioning

confidence: 99%

The Role of MicroRNAs in Phosphorus Deficiency Signaling

2011

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“…The remaining miRNAs found unique upon glyphosate treatment, including miR159, miR166, miR167, miR169 and miR396 were also shown to be differentially expressed under Pi starvation in Arabidopsis, wheat, maize, soybean and white lupin (Lundmark et al 2010;Pei et al 2013;Zeng et al 2010;Zhao et al 2013;Zhu et al 2010). …”

Section: Discussionmentioning

confidence: 99%

Expression of miRNAs involved in phosphate homeostasis and senescence is altered in glyphosate-treated maize

et al. 2015

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Glyphosate is a systemic, nonselective and most widely used herbicide in the world. The introduction of glyphosate-resistant crops in the mid-1990s resulted in a dramatic increase in the use of glyphosate herbicide making it most widely used herbicide in the world. The average maize yield loss in the field caused by pests is around 20 % but in many regions it is much higher. It is now clear that glyphosate causes broader range of physiological alterations than previously assumed and some plants gain higher level of resistance to glyphosate without the need to use genetic engineering methods. To understand the mechanisms of such heightened resistance we must first know the processes mediating the plants' death in response to glyphosate treatment. Here, we show that 12 miRNAs, belonging to miR167, miR396, miR159, miR156, miR169, miR444 and miR827 families, are significantly upregulated, and one, miR166, downregulated following glyphosate treatment. These miRNAs have been previously shown to be involved in abiotic stress responses and implicated in senescence. Strikingly, two of the induced miRNAs, miR444 and miR827, have been shown to regulate phosphate transport pathways, which seem to be common for Pi and glyphosate uptake.

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Analysis of phosphorus-deficient responsive miRNAs and cis-elements from soybean (Glycine max L.)

Cited by 96 publications

References 56 publications

Regulation of miR399f Transcription by AtMYB2 Affects Phosphate Starvation Responses in Arabidopsis

Regulation of miR399f Transcription by AtMYB2 Affects Phosphate Starvation Responses in Arabidopsis

The Role of MicroRNAs in Phosphorus Deficiency Signaling

Expression of miRNAs involved in phosphate homeostasis and senescence is altered in glyphosate-treated maize

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