The Role of MicroRNAs in Phosphorus Deficiency Signaling

Kuo, Hui‐Fen; Chiou, Tzyy‐Jen

doi:10.1104/pp.111.175265

Cited by 149 publications

(114 citation statements)

References 60 publications

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“…Hsieh et al (2009) also showed that phosphate deficiency increases the upregulation of PAP/MYB75, leading to higher levels of TAS4-siR81 (-) via the activation of miRNA828, which in turn suppressed the expression of MYB transcription factors. Therefore, it was suggested that under phosphate deficiency a mechanistic cross talk could modulate anthocyanin biosynthesis in Arabidopsis shoots (Hsieh et al 2009;Kuo and Chiou 2011).…”

Section: Induction Mechanism Under Drought Stressmentioning

confidence: 99%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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Main conclusion ABA is involved in anthocyanin synthesis through the regulation of microRNA156, augmenting the level of expression of anthocyanin synthesisrelated genes and, therefore, increasing anthocyanin level.Drought stress is the main cause of agricultural crop loss in the world. However, plants have developed mechanisms that allow them to tolerate drought stress conditions. At cellular level, drought stress induces changes in metabolite accumulation, including increases in anthocyanin levels due to upregulation of the anthocyanin biosynthetic pathway. Recent studies suggest that the higher anthocyanin content observed under drought stress conditions could be a consequence of a rise in the abscisic acid (ABA) concentration. This plant hormone crosses the plasma membrane by specific transporters, and it is recognized at the cytosolic level by receptors known as pyrabactin resistance (PYR)/regulatory component of ABA receptors (PYR/RCARs) that regulate downstream components. In this review, we discuss the hypothesis regarding the involvement of ABA in the regulation of microRNA156 (miRNA156), which is upregulated as part of dehydration stress responsiveness in different species. The miRNA156 upregulation produces a greater level of anthocyanin gene expression, forming the multienzyme complex that will synthesize an increased level of anthocyanins at the cytosolic face of the rough endoplasmic reticulum (RER). After synthesis, anthocyanins are transported from the RER to the vacuole by two possible models of transport: (1) membrane vesicle-mediated transport, or (2) membrane transporter-mediated transport. Thus, the aim was to analyze the recent findings on synthesis, transport and the possible mechanism by which ABA could increase anthocyanin synthesis under drought stress conditions potentially throughout microRNA156 (miRNA156).

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Section: Induction Mechanism Under Drought Stressmentioning

confidence: 99%

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

González‐Villagra

Kurepin

2017

Planta

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“…These miRNAs are also responsive to other nutrients and abiotic stresses such as N, copper, sulfur, and drought, suggesting stress that signal transduction pathways interconnect with each other (Kuo and Chiou 2011). Elucidation of their specific role in P-metabolism requires further work.…”

Section: Micro Rnas (Mirnas) and Targetsmentioning

confidence: 99%

“…The miRNAs function as riboregulators for many downstream genes involved in P starvation responses (PSR) and also act as signal-transmitting molecules for systemic adaptive responses (Kuo and Chiou 2011). The MiR399 plays an important role in maintaining Pi homeostasis at the level of Pi acquisition, distribution, and remobilisation.…”

Section: Micro Rnas (Mirnas) and Targetsmentioning

confidence: 99%

“…The MiR399 plays an important role in maintaining Pi homeostasis at the level of Pi acquisition, distribution, and remobilisation. Upon P deficiency, miR399 acts as a positive regulator to promote Pi uptake and root-toshoot translocation of Pi (Kuo and Chiou 2011). The target gene of miR399 encodes a ubiquitin-conjugating E2 enzyme, PHO2/ UBC24, which functions mainly in the roots to regulate Pi acquisition and root-to-shoot Pi translocation (Aung et al 2006).…”

Section: Micro Rnas (Mirnas) and Targetsmentioning

confidence: 99%

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Genetic approaches to enhancing phosphorus-use efficiency (PUE) in crops: challenges and directions

2013

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Abstract. Many soils have intrinsically low concentrations of available phosphorus (P), which is a major limitation to crop and pasture growth. Regular applications of P have underpinned agricultural productivity internationally, and fertiliser use now constitutes one of the largest variable input costs to farming. Globally, high-quality reserves of P are being depleted and price increases are likely in the future. In addition, the effects of P pollution on water quality are attracting legislative regulation. Hence, there is a need to improve P-use efficiency (PUE) in farming systems.Progress in improving PUE has been limited for several reasons, including: inconsistent definitions of PUE, inappropriate phenotyping, incomplete understanding of the controls of P uptake, lack of field validation, and little consideration of genotype Â environment interactions that affect the expression of PUE. With greater consideration of these limitations, the powerful array of molecular and genomic tools currently available promises considerable advances in developing more P-efficient crops. Stronger interaction between molecular science and the traditional disciplines of plant breeding, crop physiology, soil science, and agronomy will allow new opportunities to study genetic differences in PUE, bringing P-efficient crops closer to reality.

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“…Similarly, miR399 plays an important role during inorganic phosphate (Pi) starvation by down regulating PHO2/UBC24 (a gene encoding a putative ubiquitin conjugating enzyme) mRNA 11 . PHO2 maintains proper levels of phosphate transporter activities 12 and its down regulation increases phosphate acquisition. These examples demonstrate that mere transcriptional regulation is not sufficient to achieve proper spatial and temporal expression of target genes and miRNA regulation is crucial for additional fine-tuning of gene expression.…”

mentioning

confidence: 99%

Quantitative Amplification of Cleaved Ends (qACE) to assay miRNA-directed target cleavage

et al. 2014

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microRNA (miRNA) regulation is crucial to achieve precise spatio-temporal expression patterns of their target genes. This makes it crucial to determine the levels of cleavage of a particular target mRNA in different tissues and under different conditions. We developed a quantitative PCR method "quantitative Amplification of Cleaved Ends (qACE)" to assay levels of specific cleavage products in order to determine the extent of miRNA regulation for a specific target gene. qACE uses cDNA generated from adapter-ligated RNA molecules and relies on a carefully designed fusion primer that spans the adapter-cleaved RNA junction in qPCR to specifically amplify and quantify cleaved products. The levels of full-length transcripts can also be assayed in the same cDNA preparation using primers that span across the miRNA cleavage site. We used qACE to demonstrate that soybean roots over-expressing miR164 had increased levels of target cleavage and that miRNA deficient Arabidopsis mutants had reduced levels of target cleavage. We used qACE thaliana hen1-1 to discover that differential cleavage by miR164 in nodule adjacent root vs. tissue contributed to nodule-specific expression of NAC1 transcription factors in soybean. These experiments show that qACE can be used to discover and demonstrate differential cleavage by miRNAs to achieve specific spatio-temporal expression of target genes in plants.

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The Role of MicroRNAs in Phosphorus Deficiency Signaling

Cited by 149 publications

References 60 publications

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

Evaluating the involvement and interaction of abscisic acid and miRNA156 in the induction of anthocyanin biosynthesis in drought-stressed plants

Genetic approaches to enhancing phosphorus-use efficiency (PUE) in crops: challenges and directions

Quantitative Amplification of Cleaved Ends (qACE) to assay miRNA-directed target cleavage

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