Analysis of phenolic compounds in Epimedium plants using liquid chromatography coupled with electrospray ionization mass spectrometry

Zhao, Haiyu; Sun, Jianghao; Fan, Miao-Xuan; Li, Fan; Zhou, Lei; Zhi, Li; Han, Jian; Wang, Baorong; Guo, Dan

doi:10.1016/j.chroma.2008.02.109

Cited by 86 publications

(68 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The COSY spectra showed cross peaks indicating direct 2D-shift-correlations between these hydrogen signals. Singlet signal at δH 12.5 was attributed to the hydroxyl group at carbon atom C-5, and the presence of a carbonyl group at carbon atom C-4 was confirmed by 13 − caused by the cleavage of isopentenyl [23] . …”

Section: Resultsmentioning

confidence: 98%

Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras

Farhan

Rammal

Hijazi

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 98%

Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras

Farhan

Rammal

Hijazi

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

“…As the major bioactive constituents of Epimedium spp. plants, the total flavonoids showed androgenic, antioxidant, antidepressant, antiosteoporosis, and antitumor activities (Wu et al, 2012;Zhao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

The growth and medicinal quality ofEpimedium wushanenseare improved by an isolate of dark septate fungus

Zhu

Fan

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Seven dark-septate endophytic (DSE) fungi have been isolated from the roots of Epimedium wushanense T. S. Ying (Berberidaceae), an important medicinal plant with various pharmacological activities. Objective: The current study explores the effects of seven DSE fungi on the growth and accumulation of bioactive compounds in E. wushanense. Materials and methods: Each 1-month-old E. wushanense seedling was inoculated with one of the seven DSE fungi and was grown under greenhouse conditions for 90 d. The molecular identification of the fungi was based on the ITS1-5.8S-ITS2 nuclear ribosomal gene cluster. Results:The results showed that the influence of DSE fungi inoculation varied between strains. Inoculation with DSE8 not only significantly enhanced plant height, root length, leaf area, leaf number, and shoot and root biomass but also improved the total flavonoid and icariin content, with an increase ranging from 20.24% to 237.97%. Three of the seven DSE fungi caused the inoculated plants to die, and the remaining three DSE strains showed neutral or negative effects on plant growth and the accumulation of bioactive compounds. According to the ITS sequence, DSE8 is congeneric to the genus Leptodontidium. Discussion and conclusion: The findings indicate that application of DSE8 may be valuable to facilitate the cultivation of E. wushanense with a higher biomass and improved medicinal quality.

show abstract

“…According to their retention time (RT), accurate mass measurement and literature reports [21], they were identified in accordance with their chromatographic peaks in the BPC which are shown in Fig. 1c.…”

Section: Identification Of Four Target Compoundsmentioning

confidence: 84%

“…were identified rapidly by TOFMS within 30 min. Focus is on the major flavonoids (not all components) in Epimedium in vitro, the method is faster and more effective compared with the other literatures [19][20][21]，which need 1 h or even longer analysis time. In the 31 common components, epimedin A (15), epimedin B (16), epimedin C (17) and icariin (19) are still the main ingredients with a high content in Epimedium which were always used as a basis for quality control, consistent with the reported literature [1].…”

Section: Differentiation Of Components In Two Kinds Of Epimedium Extractmentioning

confidence: 90%

“…It can also distinguish isomers according to their characteristic fragmentations [16][17][18]. There were already some reports about compounds analysis in Herba Epimedii by LC/MS methods, but little attention has been devoted to the composition difference between the species in vitro and metabolic changes of flavonoids in vivo [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and differentiation of major flavonoids in two kinds ofEpimediumextracts and rat plasma by rapid-resolution liquid chromatography combined with time-of-flight mass spectrometry

Zhao

Wang

et al. 2015

Acta Chromatographica

View full text Add to dashboard Cite

Summary. Epimedium pubescens Maxim. and Epimedium koreanum Nakai. are two common and confused species of Herba Epimedii in Chinese Pharmacopoeia 2010 edition. Different species and growing conditions lead to chemical differences between the two species which may result in the improper clinical usage. In this work, a new method based on rapid-resolution liquid chromatography combined with time-of-flight mass spectrometry (RRLC/TOFMS) has been developed for identification and differentiation of major flavonoids in two kinds of Epimedium extract and rat plasma. The compounds were identified effectively based on the accurate extract masses and formulae acquired by RRLC/TOFMS. The fragmentation rules deduced by collision-induced dissociation (CID) were successfully implemented in distinguishing some of the isomers, further validating the results. By using the combined analytical techniques, a total of 40 major flavonoids in extracts of two kinds of Epimedium were identified within 30 min, including 31 common components and 9 characteristic components. After oral administration, three prototype compounds in rat plasma were detected by comparing the constituents measured in vitro with those in vivo, and five metabolites were identified by contrasting the fragmentation rules. The identification and structural elucidation of the chemical constituents provided essential data for further pharmacological and clinical studies on different species of Epimedium.

show abstract

Analysis of phenolic compounds in Epimedium plants using liquid chromatography coupled with electrospray ionization mass spectrometry

Cited by 86 publications

References 34 publications

Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras

Chemical composition and antioxidant activity of a Lebanese plant Euphorbia macroclada schyzoceras

The growth and medicinal quality ofEpimedium wushanenseare improved by an isolate of dark septate fungus

Identification and differentiation of major flavonoids in two kinds ofEpimediumextracts and rat plasma by rapid-resolution liquid chromatography combined with time-of-flight mass spectrometry

Contact Info

Product

Resources

About