Analysis of Pfcrt, Pfmdr1, Dhfr, and DHPS Mutations and Drug Sensitivities in Plasmodium Falciparum Isolates From Patients in Vietnam Before and After Treatment With Artemisinin

Ngo, Thanh; Duraisingh, Manoj T.; Reed, Michael B.; Hipgrave, David; Biggs, Beverley‐Ann; Cowman, Alan F.

doi:10.4269/ajtmh.2003.68.350

Cited by 62 publications

(51 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo resistance to QN was confirmed by an in vitro test and by molecular identification of an mdr1 Tyr-86 point mutation. The direct correlation between the presence of that mutation and QN resistance has been reported by Duraisingh et al (1), although it is not consistently found (7,9). As expected, in vitro testing showed CQ resistance, in line with the presence of mutated alleles at codons 76 and 220 of the pfcrt gene.…”

Section: Vol 42 2004 Case Reports 5425mentioning

confidence: 79%

Genetic Confirmation of Quinine-Resistant Plasmodium falciparum Malaria Followed by Postmalaria Neurological Syndrome in a Traveler from Mozambique

et al. 2004

View full text Add to dashboard Cite

A case of quinine-resistant Plasmodium falciparum malaria, followed by a postmalaria neurological syndrome and a recurrence episode, is described. Genetic characterization of the P. falciparum isolate obtained by analysis of msp1 and msp2 amplicons revealed the coexistence of two genotypes causing the first malaria episode and the presence of a unique isolate responsible for the recurrence. CASE REPORTFifteen days after returning from Bilene (Maputo Province, Mozambique), a previously healthy 42-year-old man was admitted to the National Institute for Infectious Diseases L. Spallanzani, Rome, Italy, with a 4-day history of headache and febrile illness. Plasmodium falciparum malaria was diagnosed on the basis of blood film examination; the initial level of parasitemia was Ͼ100,000 parasites/l. The patient had not taken antimalarial prophylaxis during a business trip to Mozambique. On examination, the patient was fully conscious and had a temperature of 38°C. Hepatosplenomegaly was detected. Acute complications included hemolysis and severe thrombocytopenia. The patient was treated with intravenous (i.v.) quinine (QN; 20 mg of the hydrochloride salt per kg initially and then 10 mg/kg three times a day) plus oral doxycycline (100 mg two times a day). The parasitemia was cleared by day 4, whereupon the patient was treated with oral QN (8 mg of base/kg three times daily) plus doxycycline until day 8. He was discharged from the hospital on day 9.Nine days later, however, the patient developed a low-grade fever with acute confusion (inappropriate speech and markedly disturbed behavior), postural tremor, and nominal aphasia. He was readmitted to our hospital on day 20. On examination, the patient had a temperature of 38.2°C with no clinically detectable focus of infection and without meningism. He was in an acute confusional state with nominal aphasia and showed a fine postural tremor of the arms that worsened when he tried to move his arms. The lowest Glasgow coma score was 12. No abnormalities were found in the cardiovascular and respiratory systems. No previous history of neurological or psychiatric illness was ascertained. No medication had been taken by the patient at home. Simultaneous thick and thin blood film tests on 3 different days were negative for malarial parasites. Computerized tomography and gadolinium-enhanced T 1 -and T 2 -weighted magnetic resonance imaging scans of the brain were also normal except for the presence of maxillary sinus exudate. On day 20, treatment with i.v. ceftriaxone (2,000 mg once a day) was begun. Analysis of a cerebrospinal fluid sample obtained by lumbar puncture revealed mild lymphocytic pleocytosis (45 lymphocytes/l), a normal glucose concentration, and an elevated protein concentration of 1.29 g/liter (normal range, 0.2 to 0.4 g/liter). Pending herpes simplex virus testing, i.v. acyclovir (10 mg/kg three times a day) was empirically added to the treatment regimen on day 24 and stopped on day 28. Subsequent tests of cerebrospinal fluid for viral, bacterial, and fungal infection...

show abstract

Section: Vol 42 2004 Case Reports 5425mentioning

confidence: 79%

Genetic Confirmation of Quinine-Resistant Plasmodium falciparum Malaria Followed by Postmalaria Neurological Syndrome in a Traveler from Mozambique

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Wild-type N86 allele in Pfmdr1 has been found with increased tolerance to both artemether and lumefantrine separately. [52][53][54] Moreover, the presence of N86 wild-type allele is a probable factor in the development of resistance against lumefantrine, and frequency of this allele varies according to different malarial transmission settings. 55,56 Pfmdr1 Y184F allele has either minor impact on drug response or weaker association with antimalarial effectiveness as compared with N86Y mutation, and expression of wild-type or mutant allele in codon 184 primarily depends on codon 86 status, class of drug to which parasite expose and genetic background of Pfcrt gene.…”

Section: Discussionmentioning

confidence: 99%

K13 Kelch Propeller Domain and mdr1 Sequence Polymorphism in Field Isolates From Northeast Region, India

Dutta¹,

Chetry²,

Kalita³

et al. 2017

HPD

View full text Add to dashboard Cite

Emergence of artemisinin-resistant Plasmodium falciparum in Southeast Asia has made an intense warning to global malaria control programme since Southeast Asia is playing a key role in the evolution of drug resistant malaria parasites. Several polymorphisms in K13 gene have been designated with developing resistance either in vitro or in vivo. In this study, polymorphisms within K13 kelch propeller domain and Pf mdr1 gene were investigated in a total of 134 P. falciparum field isolates circulating in 3 states of Northeast region of India, namely, Assam, Arunachal Pradesh, and Tripura because information regarding this is limited from this region. Six polymorphisms were detected, out of which 2 were new. A481V mutation was found in 4.5% of the total field isolates. A675V and D702N new mutations were present in 3.7% and 1.5% isolates, respectively. Synonymous polymorphism at codon 703 was highly observed (6.7%) than other polymorphisms. N86Y allele in Pf mdr1 gene was also noticed in isolates that contained mutation in K13 propeller domain, but N86 wild-type allele was found comparatively higher in frequency within overall isolates. Moreover, both nucleotide and haplotype diversities in K13 gene were high in Arunachal Pradesh isolates than other 2 states. This is the first report from Northeast region of India with evidence of A675V candidate mutation along with 2 other new mutations which pays attention for further molecular surveillance in this region to document detailed scenario of K13 polymorphism pattern corresponding to artemisinin resistance.

show abstract

“…Since the site of the Cambodian study was within close proximity to our study site, these isolates may have been generated by low-level clonal spread across these areas. In Vietnam, Ngo (14).…”

Section: Discussionmentioning

confidence: 99%

“…In Vietnam, chloroquine-resistant P. falciparum was reported for the first time in the 1960s (12,14). Ngo et al reported in 2003 that all of the isolates acquired from 18 adult rubber plantation workers residing in southern Vietnam demonstrated the pfcrt 76T mutation (14).…”

mentioning

confidence: 99%

“…Ngo et al reported in 2003 that all of the isolates acquired from 18 adult rubber plantation workers residing in southern Vietnam demonstrated the pfcrt 76T mutation (14). In contrast, Phuc et al reported that the prevalence of the mutant was only 38.5% when the strains from 39 malaria patients in the Quang Tri Province of central Vietnam were investigated (15).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Longitudinal Survey of Plasmodium falciparum Infection in Vietnam: Characteristics of Antimalarial Resistance and Their Associated Factors

et al. 2010

View full text Add to dashboard Cite

Plasmodium falciparum is the main cause of human malaria and is one of the important pathogens causing high rates of morbidity and mortality. The total number of malaria patients in Vietnam has gradually decreased over the last decade. However, the spread of pathogens with drug resistance remains a significant problem. Defining the trend in genotypes related to drug resistance is essential for the control of malaria in Plasmodium falciparum has long been one of the most important pathogens, causing severe illness and large numbers of deaths worldwide. In the 1990s, more than 1 million people living in Vietnam suffered from P. falciparum infections, resulting in thousands of deaths per year. Since then, the National Institute of Malariology, Parasitology, and Entomology (NIMPE) and the government of Vietnam have focused a great deal of time and effort on a malaria control program. As a result,

show abstract

Analysis of Pfcrt, Pfmdr1, Dhfr, and DHPS Mutations and Drug Sensitivities in Plasmodium Falciparum Isolates From Patients in Vietnam Before and After Treatment With Artemisinin

Cited by 62 publications

References 39 publications

Genetic Confirmation of Quinine-Resistant Plasmodium falciparum Malaria Followed by Postmalaria Neurological Syndrome in a Traveler from Mozambique

Genetic Confirmation of Quinine-Resistant Plasmodium falciparum Malaria Followed by Postmalaria Neurological Syndrome in a Traveler from Mozambique

K13 Kelch Propeller Domain and mdr1 Sequence Polymorphism in Field Isolates From Northeast Region, India

Longitudinal Survey of Plasmodium falciparum Infection in Vietnam: Characteristics of Antimalarial Resistance and Their Associated Factors

Contact Info

Product

Resources

About