Analysis of organophosphorus pesticides in whole blood by GC-MS-μECD with forensic purposes

Valente, Nuno I.P.; Tarelho, Sónia; Castro, André L.; Silvestre, Armando J. D.; Teixeira, Helena M.

doi:10.1016/j.jflm.2015.03.006

Cited by 45 publications

(22 citation statements)

References 25 publications

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“…Despite the advantage possessed by blood specimens, most researchers tend to collect urine, instead of blood, for biomonitoring probably because it is an easy and non-invasive procedure. There are a few studies focusing on blood sample analysis for insecticides [34][35][36]; the results, however, are non-comparable to that of studies using urine sampling and vice versa. Little is known about the relation between insecticide data derived from blood and urine, although it is presumably present.…”

Section: Introductionmentioning

confidence: 91%

Relationship between Organophosphate and Pyrethroid Insecticides in Blood and Their Metabolites in Urine: A Pilot Study

Simaremare

Hung

Hsieh

et al. 2019

IJERPH

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We conducted a pilot study to examine the relationship between organophosphate (OP) and pyrethroid (PYR) insecticides in blood and their metabolites in urine. A total of 30 pregnant women were enrolled in the study, and blood and urine was sampled from each subject during a regular clinic visit. Two OP and nine PYR insecticides were selected for blood sample analysis, while six OP and five PYR metabolites were analyzed for urine specimens. Both types of samples were processed and analyzed on gas chromatography-mass spectrometry. For OPs in blood, chlorpyrifos had a higher mean concentration (73.33 µg/L) than terbufos. For PYRs in blood, cypermethrin and imiprothrin were the most frequently detected species with the highest mean concentrations (151.25 and 141.25 µg/L). The concentrations of PYRs appeared to be higher than that of OPs, and the most frequently detected PYRs were commonly used in domestic products, suggesting that the exposure could mostly originate from use of domestic insecticides. The correlation between insecticides in blood and their metabolites in urine was significantly high (r = 0.795 for OPs and 0.882 for PYRs, p < 0.001), indicating routine exposure at a steady state. Residents should be cautious with domestic use of insecticide products to lower their exposure.

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Section: Introductionmentioning

confidence: 91%

Relationship between Organophosphate and Pyrethroid Insecticides in Blood and Their Metabolites in Urine: A Pilot Study

Simaremare

Hung

Hsieh

et al. 2019

IJERPH

View full text Add to dashboard Cite

show abstract

“…Several articles have been published using different work‐up procedures designed for the detection of different analytes of interest, and a wide variety of substances can be detected in blood or also in urine in a single analysis . In some cases, there is no previous knowledge about the poisoning history of the patient.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9][10][11] Several articles have been published using different work-up procedures designed for the detection of different analytes of interest, and a wide variety of substances can be detected in blood or also in urine in a single analysis. [12][13][14][15][16][17][18] In some cases, there is no previous knowledge about the poisoning history of the patient. Then the preliminary screening procedures are necessary for the identification of the possible substance(s) involved.…”

mentioning

confidence: 99%

A fast and simple approach for the quantification of 40 illicit drugs, medicines, and pesticides in blood and urine samples by UHPLC‐MS/MS

Oliveira

Zucoloto

Oliveira³

et al. 2019

J Mass Spectrom

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A fast and simple approach to overcome challenges in emergency toxicological analysis, using ultra‐high performance liquid chromatography–tandem mass spectrometry (UHPLC‐MS/MS) has been developed, for the detection of analytes in blood and urine samples from the following drug classes: analgesics, benzodiazepines, antidepressants, anticonvulsants, drugs of abuse, and pesticides. These substances are relevant in the context of emergency toxicology in Brazil. The sample preparation procedure was relatively easy and fast to perform. The method was fully validated giving limits of in the range of 0.5 and 20 ng mL−1 for blood and urine samples. The intraday and interday precision and accuracy were considered adequate for all analytes once the relative standard deviation (RSD) (%) was lower than 20% for quality control (QC) low and lower than 15% for CQ medium and high. The developed method was successfully applied to 320 real samples collected at the Poison Control Center of São Paulo, and 89.1% have shown to be positive for some of the analytes. This confirms its applicability and importance to emergency toxicological analysis, and it could be very useful in both fields of clinical and forensic toxicology.

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“…Among the analyzed pesticides, organophosphate pesticides, which account for over 38% of totally used pesticides, have been targets for decades due to their main contribution and toxicity. 2,3 Conventional pesticide analysis mainly relies on UV-HPLC, 4,5 GC-MS, 6 fluorescent and chemiluminescent assays. 7,8 However, these methods require bulky instruments together with fully equipped laboratory infrastructure and well-trained operators.…”

Section: Introductionmentioning

confidence: 99%

Flow Control-based 3D μPADs for Organophosphate Pesticide Detection

et al. 2018

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Flow control-based paper devices have recently shown great potential for point-of-need analysis, since they allow for the easy operation of multi-step assays by minimizing user operation. In this work, a wax printing method was evaluated as a means to control liquid flow in 3D microfluidic paper-based analytical devices (μPADs). The resulting flow controlbased 3D μPADs were applied to determine paraoxon-ethyl as a typical organophosphate pesticide model system. The analytical procedure is as simple as applying a 200-μL sample solution, resulting in reproducible (relative standard deviation of colorimetric signals from 6 independently fabricated devices, 2.63%) colorimetric signals within 1 h of the assay time with the limit of detection (LOD) reaching 25.0 μg/L. Finally, results obtained for pesticide-spiked water samples analyzed by flow control-based 3D μPADs showed good agreement with those from a conventional HPLC analysis with UV detection.

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Analysis of organophosphorus pesticides in whole blood by GC-MS-μECD with forensic purposes

Cited by 45 publications

References 25 publications

Relationship between Organophosphate and Pyrethroid Insecticides in Blood and Their Metabolites in Urine: A Pilot Study

Relationship between Organophosphate and Pyrethroid Insecticides in Blood and Their Metabolites in Urine: A Pilot Study

A fast and simple approach for the quantification of 40 illicit drugs, medicines, and pesticides in blood and urine samples by UHPLC‐MS/MS

Flow Control-based 3D μPADs for Organophosphate Pesticide Detection

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