Analysis of Oligonucleotide Probe Affinities Using Surface Plasmon Resonance: A Means for Mutational Scanning

Persson, Björn; Stenhag, Karin; Nilsson, Peter; Larsson, Anita; Uhlén, Mathias; Nygren, Per‐Åke

doi:10.1006/abio.1996.9988

Cited by 89 publications

(55 citation statements)

References 21 publications

(8 reference statements)

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“…3 and 4) were performed with 75-l injections at a slightly elevated temperature (14°C) to reduce the time to reach hybridization equilibrium. The temperatures were chosen in accordance with results from previous experiments (22) involving hybridizations of short oligonucleotides, to achieve fast interactions and enabling analysis of reliable and significant equilibrium responses.…”

Section: Methodsmentioning

confidence: 98%

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Quantitative Investigation of the Modular Primer Effect for DNA and Peptide Nucleic Acid Hexamers

Nilsson

O’Meara

Edebratt

et al. 1999

Analytical Biochemistry

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Section: Methodsmentioning

confidence: 98%

“…Due to the fast interaction kinetics of hexamer hybridizations, relatively short interaction times are needed to reach steady-state levels, suitable for determination of apparent affinities using nonlinear fitting of equilibrium responses (22).…”

Section: Biosensor System Setupmentioning

confidence: 99%

Quantitative Investigation of the Modular Primer Effect for DNA and Peptide Nucleic Acid Hexamers

Nilsson

O’Meara

Edebratt

et al. 1999

Analytical Biochemistry

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“…Biosensor technology has recently been shown to be an attractive approach to study nucleic acid interactions (11)(12)(13)(14)(15), allowing kinetic evaluation of the hybridization properties of oligonucleotides (16,17) as well as facilitating the application of this technique to mutation analysis of PCR products (18). The detection principle in the Biacore system relies on the use of surface plasmon resonance (SPR 2 ) to measure changes in the refractive index at a sensor surface.…”

mentioning

confidence: 99%

Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules

O’Meara

Nilsson

Nygren

et al. 1998

Analytical Biochemistry

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“…Here several fiber-optic DNA biosensors based surface plasmon resonance (SPR) [2,49,50,51], ellipsometry, evanescent wave are depicted below.…”

Section: Label-free Fiber-optic Dna Biosensorsmentioning

confidence: 99%

Recent advances in fiber-optic DNA biosensors

Wang¹,

Pang²,

Zhang³

2009

JBiSE

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Fiber-optic DNA biosensors are a kind of analytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen's triplex (T/A-T, C/G-C) formation into a readable analytical signals when functionalized singlestrands DNA (ssDNA) or double-strands DNA (dsDNA) of interest are immobilized on the surface of fiber-optic hybrids with target DNA or interacts with ligands. This review will provide the information about the fiber-optic DNA biosensors classified into two categories depending on the end fiber and side fiber with or without the labels-label-free fiber-optic DNA biosensors and labeled fiber-optic DNA biosensor in recent years. Both are dissertated, and emphasis is on the label-free fiber-optic DNA biosensors. Fiber-optic DNA biosensors had got great progresses because fiber-optic has more advantages over the other transducers and are easily processed by nanotechnology. So fiberoptic DNA biosensors have increasingly attracted more attention to research and develop the new fiber-optic DNA biosensors that integrated with the "nano-bio-info" technology for in vivo test, single molecular detection and on-line medical diagnosis. Finally, future prospects to the fiber-optic DNA biosensors are predicted.

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Analysis of Oligonucleotide Probe Affinities Using Surface Plasmon Resonance: A Means for Mutational Scanning

Cited by 89 publications

References 21 publications

Quantitative Investigation of the Modular Primer Effect for DNA and Peptide Nucleic Acid Hexamers

Quantitative Investigation of the Modular Primer Effect for DNA and Peptide Nucleic Acid Hexamers

Capture of Single-Stranded DNA Assisted by Oligonucleotide Modules

Recent advances in fiber-optic DNA biosensors

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