Analysis of NK Cells in Peripheral Blood and Tumor Infiltrating Lymphocytes in Cervical Cancer Patients

Cespedes, Maria A.; Rodríguez, Josefa Antonia; Medina, Mónica; Bravo, María Mercedes; Cómbita, Alba Lucía

doi:10.1016/s0123-9015(12)70027-6

Cited by 7 publications

(9 citation statements)

References 37 publications

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“…We speculated that our test sample and method may have led to different results. Studies have reported that the percentage of NK cells in the peripheral blood was not inconsistent between the core of the lung cancer [30] and cervical cancer [31]. We also observed that the TNM stage had no significant association with the survival of CRC patients.…”

Section: Discussioncontrasting

confidence: 44%

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Tang

Xie

et al. 2020

BMC Gastroenterol

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Background: The association between natural killer (NK) cells and survival in colorectal cancer (CRC) patients remains controversial. This study aimed to clarify the prognostic value of peripheral blood NK cells in CRC patients.Methods: A total of 447 CRC patients who underwent radical surgery and chemotherapy were retrospectively analyzed. Cox regression analyses were used to identify independent prognostic indicators. Correlation between NK cell percentage and other clinicopathological features (gender, age, histological grade, tumor stage, immune cells, and inflammatory indicators) was analyzed. The prognostic values of the combinations of NK cell percentage and other clinicopathological features were also determined. Results: Multivariate Cox regression analysis revealed that NK cell percentage in the peripheral blood was an independent prognostic indicator in CRC patients. A higher percentage of NK cells indicated a longer survival time than a lower percentage. NK cell percentage was positively correlated to the T and B lymphocyte counts and negatively correlated to the patients' age and albumin levels. With an area of 0.741 under a receiver operating characteristic curve, NK cells have a moderate predictive value for 3rd-year survival in CRC. This area increased to 0.851 by combining NK cell percentage with the B lymphocyte count. Elderly patients and those at an advanced clinical stage presented a lower percentage of NK cells than younger patients and those at an early clinical stage.Conclusions: This study demonstrated that NK cells in the blood were an independent predictor of survival in CRC patients, and the combined count of NK cells and B lymphocytes could increase the prognostic value.

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Section: Discussioncontrasting

confidence: 44%

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Tang

Xie

et al. 2020

BMC Gastroenterol

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“…However, similar to other cancers, cervical tumors and advanced dysplasias have also been characterized by various ‘dysfunctions’ of the immune system; that is, failure of immunosurveillance [21]. Interestingly, while down‐regulated MHC‐I has been found in cervical cancer and increased NK cell percentages have been found in patient blood, this increase was not reflected in the tumor, suggesting that this inefficient migration may be due to inhibition of the NK cells [22]. NK cell activity in the HPV‐infected cervix and cervical cancer may be affected in different ways [23,24].…”

Section: Introductionmentioning

confidence: 99%

Immune checkpoint expression on peripheral cytotoxic lymphocytes in cervical cancer patients: moving beyond the PD-1/PD-L1 axis

Solorzano‐Ibarra

Alejandre-Gonzalez

Ortiz‐Lazareno

et al. 2021

Clinical and Experimental Immunology

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Summary Immune checkpoint therapy to reverse natural killer (NK) and T cell exhaustion has emerged as a promising treatment in various cancers. While anti‐programmed cell death 1 (PD‐1) pembrolizumab has recently gained Food and Drug Administration (FDA) approval for use in recurrent or metastatic cervical cancer, other checkpoint molecules, such as T cell immunoreceptor with immunoglobulin (Ig) and immunoreceptor tyrosine‐based inhibition motif (ITIM) domains (TIGIT) and T cell immunoglobulin and mucin‐domain containing‐3 (Tim‐3), have yet to be fully explored in this disease. We report expression of TIGIT, Tim‐3 and PD‐1 on subsets of peripheral blood NK (CD56dim/negCD16bright/dim/neg and CD56brightCD16dim/neg) and T cells. The percentages of these cells were increased in women with cervical cancer and pre‐malignant lesions. PD‐1+ NK and T cells were likely to co‐express TIGIT and/or Tim‐3. These cells, with an apparently ‘exhausted’ phenotype, were augmented in patients. A subset of cells were also natural killer group 2 member D (NKG2D)‐ and DNAX accessory molecule 1 (DNAM‐1)‐positive. PD‐1int and PD‐1high T cells were notably increased in cervical cancer. Soluble programmed cell death ligand 1 (PD‐L1) was higher in cancer patient blood versus healthy donors and we observed a positive correlation between sPD‐L1 and PD‐1+ T cells in women with low‐grade lesions. Within the cancer group, there were no significant correlations between sPD‐L1 levels and cervical cancer stage. However, when comparing cancer versus healthy donors, we observed an inverse association between sPD‐L1 and total T cells and a correlation between sPD‐L1 and CD56dim NK cells. Our results may show an overview of the immune response towards pre‐cancerous lesions and cervical cancer, perhaps giving an early clue as to whom to administer blocking therapies. The increase of multiple checkpoint markers may aid in identifying patients uniquely responsive to combined antibody therapies.

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“…27 Another study also showed that only small numbers of NK cells infiltrated tumor tissue. 28 Therefore, the role of NKs cells in eliminating tumor cells was small. NK cells seemed unable to function and infiltrate tumor tissue in patients with cancer.…”

Section: Therapeutic Responsementioning

confidence: 99%

“…NK cells seemed unable to function and infiltrate tumor tissue in patients with cancer. 28 Development of precervical cancer lesions and cervical cancers are strongly related to the change of Th1 cytokine to Th2, a response that is marked by increases of IL-4 and IL-10 and decreases of IL-12 and IFN-c. IL-10 decreases specific tumor immune responses by downregulating IFN-c and IL-12 production. These will inhibit migration and activation of NK cells and cytotoxic T-lymphocytes (CTLs).…”

Section: Therapeutic Responsementioning

confidence: 99%

Effect of Electroacupuncture on Natural-Killer Cells and Tumor Size in Patients with Cervical Squamous-Cell Carcinoma: A Randomized Controlled Trial

Saraswati

Dahlan

Saputra

et al. 2019

Medical Acupuncture

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Background: A natural-killer (NK) cell is a cytotoxic lymphocyte that responds to tumor formation. Electroacupuncture (EA) in patients with cancer, who had chemotherapy, enabled them to maintain their T-cell counts (CD3+, CD4+, and CD8+) and NK-cell activity. Objective: To evaluate NK-cell levels and tumor sizes in patients with cervical squamous-cell carcinoma (SCC) after EA on ST 36 (Zusanli). Design: This study was a randomized, experimental clinical study with a pre-post-test, control group design. Setting The study was performed in the obstetrics and gynecology department, Gynecology Oncology Division of the

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Analysis of NK Cells in Peripheral Blood and Tumor Infiltrating Lymphocytes in Cervical Cancer Patients

Cited by 7 publications

References 37 publications

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Immune checkpoint expression on peripheral cytotoxic lymphocytes in cervical cancer patients: moving beyond the PD-1/PD-L1 axis

Effect of Electroacupuncture on Natural-Killer Cells and Tumor Size in Patients with Cervical Squamous-Cell Carcinoma: A Randomized Controlled Trial

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