Analysis of Nitrite/Nitrate in Biological Fluids: Denitrification of 2-Nitropropane in F344 Rats

Sohn, Ock Soon; Fiala, Emerich S.

doi:10.1006/abio.1999.4471

Cited by 14 publications

(6 citation statements)

References 24 publications

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“…These nitrite excretion rates correspond to nitrite concentrations of 14 ± 5 M and are approximately 10-20 times higher than those measured by GC-MS [147,155,166]. The nitrite concentrations repoprted by Kurioka et al [163] are not supported by the findings of Li et al [169] who found by HPLC analysis and fluorescence detection using the DAN reagent that nitrite is present in urine of untreated rats at 682 ± 66 nM, nor by the findings of Sohn and Fiala [123] who could not measure nitrite in urine of untreated rats above 2000 nM, i.e. the LOD of the method.…”

Section: Nitrite Concentrations In Urinecontrasting

confidence: 67%

Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research

Tsikas

2007

Journal of Chromatography B

601

355

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Section: Nitrite Concentrations In Urinecontrasting

confidence: 67%

Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research

Tsikas

2007

Journal of Chromatography B

601

355

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“…Nitrite was quantified similarly but in the absence of nitrate reductase. NADPH was removed by zinc acetate precipitation (75,76). To each sample, 100 l of 0.5 M zinc acetate in 50% ethanol was added, and the samples were vortexed.…”

Section: Nitrate and Nitrite Measurement By Griess Assaymentioning

confidence: 99%

A nitric oxide synthase–like protein from Synechococcus produces NO/NO3− from l-arginine and NAPDH in a tetrahydrobiopterin- and Ca2+-dependent manner

Picciano

Crane

2019

Journal of Biological Chemistry

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“…The addition of NaNO 2 did not alter the pH of the reaction mixture significantly. After incubating the reaction mixtures at 37 °C for 60 min, the NO 2 – level in solution was determined by the Griess method, and protein tyrosine nitration was analyzed by western blotting. , …”

Section: Methodsmentioning

confidence: 99%

Key Roles for Tyrosine 10 in Aβ–Heme Complexes and Its Relevance to Oxidative Stress

Tian

et al. 2014

Chem. Res. Toxicol.

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Amyloid β-peptide (Aβ) aggregation in the brain, known as amyloid plaques, is a pathological feature of Alzheimer's disease (AD). Recent studies show that heme binds to the His residue of Aβ with the iron center and subsequently forms an Aβ-heme complex, which can inhibit Aβ aggregation. Although Tyr-10 was not the residue binding heme, the key roles for Tyr-10 in Aβ-heme complexes and its potential biological relevance to AD etiology under oxidative stress were not sufficiently evaluated. Here, we used wild-type and Tyr-10-mutated human Aβ peptides and studied the impact of the mutation on Aβ-heme peroxidase activity, heme-bound Aβ aggregation, and oxidation and nitration under oxidative and nitrative stresses. Our results indicate that the mutation of Tyr-10 in Aβ16 and Aβ40 decreased the peroxidase activity of Aβ-heme complexes and that the mutation of Tyr-10 could inhibit Aβ40 self-assembly aggregation. Under oxidative (H2O2) and nitrative (H2O2/NaNO2) stress conditions, the Aβ40-heme complexes caused oxidation and nitration of the Aβ Tyr-10 residue through promoting peroxidase-like reactions, which were different from the classic inhibitive effect of heme on Aβ aggregation. To our knowledge, this is the first time that the formation of a heme-to-protein cross-linked Aβ40-heme complex under oxidative stress has been reported; in addition, the mutation of Tyr-10 could inhibit the cross-link formation. Therefore, Tyr-10 is pivotal in Aβ-heme complexes and plays key roles in Aβ aggregation under oxidative and nitrative stresses, demonstrating a novel significance of Tyr-10 as well as Aβ-heme complexes in the pathology of AD.

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Analysis of Nitrite/Nitrate in Biological Fluids: Denitrification of 2-Nitropropane in F344 Rats

Cited by 14 publications

References 24 publications

Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research

Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research

A nitric oxide synthase–like protein from Synechococcus produces NO/NO3− from l-arginine and NAPDH in a tetrahydrobiopterin- and Ca2+-dependent manner

Key Roles for Tyrosine 10 in Aβ–Heme Complexes and Its Relevance to Oxidative Stress

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