Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research

Tsikas, Dimitrios

doi:10.1016/j.jchromb.2006.07.054

Cited by 603 publications

(372 citation statements)

References 174 publications

Supporting

Mentioning

355

Contrasting

Unclassified

Order By: Relevance

“…After 10 min of incubation at room temperature the ELISA reading at 540 nm was performed. The amount of nitrite from each sample was calculated using the absorbance linear regression equation from the known quantity of nitrite from the NaNO 2 solution (200µM to 0.097µM) (Tsikas, 2007).…”

Section: Nitric Oxide (No) Concentrationmentioning

confidence: 99%

Cycling above rather than below lactate threshold is more effective for nitric oxide release and post-exercise blood pressure reduction in individuals with type-2 diabetes

Asano

Browne

Sotero

et al. 2013

Motriz: rev. educ. fis.

View full text Add to dashboard Cite

Abstract-The purposes of this study were to analyze and compare the effects of exercise performed in different intensities, above and below lactate threshold (LT) on post-exercise blood pressure (BP) and nitric oxide (NO) responses in individuals with type 2 diabetes (T2D). For this, 11 T2D underwent the following sessions: 1) control session; 2) 20-min of moderate cycling (80% LT); and 3) 20-min of high intensity cycling (120%LT) on a cycle ergometer. Plasma NO and BP measurements were carried out at rest and at 15 and 45 min of post-sessions. When compared to rest, only the exercise session performed at 120%LT elicited an increase of NO (from 7.2 to 9.5 µM, p<0.05), as well as a decrease in systolic BP (from 126.6±7.9 to 118.7±3.9 mmHg, p<0.05) during the post-exercise period. In conclusion, the results suggest that NO release and post-exercise BP decrease are intensity-dependent for individuals with T2D.Keywords: nitric oxide, type-2 diabetes, high intensity exercise Resumo-"Ciclismo acima do limiar de lactato é mais efetivo que abaixo para liberação de óxido nítrico e redução da pressão arterial pós-exercício em indivíduos com diabetes tipo 2." Os objetivos do presente estudo foram analisar e comparar os efeitos do exercício realizado em diferentes intensidades, acima e abaixo do limiar de lactato (LL), sobre a resposta da pressão arterial (PA) e óxido nítrico (NO) em indivíduos com diabetes tipo 2 (DM2). Para tanto, 11 DM2 foram submetidos às seguintes sessões: 1) sessão controle; 2) 20 min de exercício em cicloergômetro em intensidade moderada (80%LL); e 3) 20 min de exercício em cicloergômetro em alta intensidade (120%LL). O NO plasmático e as medidas de PA foram realizadas no repouso, bem como aos 15 e 45 min do período de recuperação de todas as sessões. Quando comparado ao período de repouso, somente a sessão de exercício realizado a 120%LL promoveu aumento do NO (de 7,2 para 9,5 µM, p<0,05) e, por conseguinte, uma diminuição na pressão arterial sistólica (de 126,6±7,9 para 118,7±3,9 mmHg, p<0,05) durante o período de recuperação pós-exercício. Em conclusão, os resultados sugerem que a liberação de NO e a diminuição da PA parecem ser intensidade-dependente para indivíduos com DM2.Palavras-chaves: óxido nítrico, diabetes tipo 2, exercício de alta intensidadeResumen-"Ciclismo arriba del umbral de lactato es más efectivo que abajo para la liberación de óxido nítrico y reducción de la presión arterial después del ejercicio en individuos con diabetes tipo 2." Los objetivos del presente estudio fueron examinar y comparar los efectos del ejercicio realizado en diferentes intensidades, arriba y abajo del umbral de lactato (UL), sobre la respuesta de la presión arterial (PA) y el óxido nítrico (NO) en individuos con diabetes tipo 2 (DM2). Por lo tanto, 11 DM2 se sometieron las siguientes sesiones: 1) sesión control; 2) 20 min de ejercicio en cicloergómetro a una intensidad moderada (80%UL); y 3) 20 min de ejercicio en cicloergómetro a una intensidad alta (120%UL). El NO plasmático y las mediciones de la PA fuer...

show abstract

Section: Nitric Oxide (No) Concentrationmentioning

confidence: 99%

Cycling above rather than below lactate threshold is more effective for nitric oxide release and post-exercise blood pressure reduction in individuals with type-2 diabetes

Asano

Browne

Sotero

et al. 2013

Motriz: rev. educ. fis.

View full text Add to dashboard Cite

show abstract

“…Nitrite/nitrate concentration in RBC suspensions was quantified using a colorimetric kit (No: 78001, nitrite/nitrate calorimetric assay kit; Cayman Chemical, Ann Arbor, MI, USA); the principle of the method has been previously described (18). Blood samples were centrifuged at 800 g for 6 min to isolate plasma.…”

Section: Quantification Of Nitrite/nitrate Concentrationmentioning

confidence: 99%

Influence of tourniquet application on venous blood sampling for serum chemistry, hematological parameters, leukocyte activation and erythrocyte mechanical properties

Cengiz¹,

Ülker

Meiselman

et al. 2009

Clinical Chemistry and Laboratory Medicine

View full text Add to dashboard Cite

Background: Venous blood sampling is usually performed using a tourniquet to help locate and define peripheral veins to achieve successful and safe venipuncture. Despite widespread usage of tourniquets for venipuncture by medical and laboratory staff, very few are aware of the effects of tourniquet application on laboratory parameters. In addition, definitive guidelines regarding when and how to use a tourniquet for blood sampling are lacking. The aim of the present study was to define the optimal sampling time after tourniquet removal to avoid adverse impact on laboratory analytes. Methods: Blood oxygen and carbon dioxide partial pressure, pH, oxyhemoglobin saturation (satO 2 ), hematological parameters, serum electrolyte concentrations, erythrocyte, deformability and aggregation, leukocyte activation and nitrite/nitrate concentrations obtained 180 s after tourniquet release were compared with baseline values for 10 healthy subjects. Results: Blood gases, hematological parameters and serum electrolyte levels were not affected by the application and removal of a tourniquet. However, there were significant decreases in erythrocyte deformability at 90, 120, 180 s, and increases in erythrocyte aggregation at 5 and 30 s following removal of the tourniquet. A significant increase in granulocyte respiratory burst at 60 s was observed, confirming leukocyte activation due to application of the tourniquet. There were no significant alterations of blood nitrite/nitrate levels. Conclusions: Our blood sampling technique which mimicked the application and release of a tourniquet indicated unaltered values for routine blood gases, hematological testing and serum electrolyte levels. Conversely, hemorheological measurements can be

show abstract

“…The limit of detection of the present HPLC method for NO 2 − and NO 3 − analyses is much higher than that of the Griess colorimetric assay (limit of detection of 2 μmol.L −1 ) (Jobgen et al 2007). The limits of detection of the chemiluminescence method for NO 2 − and NO 3 − have been reported to be approximately 1.0 × 10 6 μmol.L −1 and 8.0×10 5 μmol.L −1 , respectively (Tsikas 2007). Thus, this assay is less sensitive than the RP-HPLCfluorescence method for NO 3 − analysis.…”

Section: Limit Of Detectionmentioning

confidence: 93%

Quantitative and Comparative Contents of Nitrate and Nitrite in Beta vulgaris L. by Reversed-Phase High-Performance Liquid Chromatography-Fluorescence

et al. 2015

View full text Add to dashboard Cite

The consumption of beetroot, a food rich in nitrate, has been recommended to enhance blood perfusion, restore endothelial function, and improve exercise performance. These properties may be explained by the possible effect of nitrate present in beetroot in stimulating the endogenous synthesis of nitric oxide, a potent vasodilator. However, there is limited evidence giving detailed information about how nitrate contents from beetroot used in studies have been analyzed. The purpose of this study was to evaluate nitrate and nitrite contents of beetroot from different regions of Brazil and from the USA. Nitrate and nitrite contents were quantified by using a reversed-phase high-performance liquid chromatography system with fluorescence detection. Beetroots from the USA showed the highest nitrate (31.2±0.010 mmol.L −1 ) and nitrite (0.45±0.005 mmol.L −1 ) contents when compared to beetroots from Brazil. Rio de Janeiro was the region that showed the highest nitrate content (17.1±0.020 mmol.L −1 ), while Rio Grande do Norte presented the highest nitrite content (0.13±0.010 mmol.L −1 ). The reversed-phase high-performance liquid chromatography-fluorescence method may be applied for the quantification of nitrate and nitrite contents in beetroot samples. The results of the present study demonstrate that beetroots may vary in their nitrate and nitrite contents.This data represents a useful tool to encourage researchers to analyze the nitrate and nitrite contents in beetroots utilized in interventional clinical studies.

show abstract

Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: Appraisal of the Griess reaction in the l-arginine/nitric oxide area of research

Cited by 603 publications

References 174 publications

Cycling above rather than below lactate threshold is more effective for nitric oxide release and post-exercise blood pressure reduction in individuals with type-2 diabetes

Cycling above rather than below lactate threshold is more effective for nitric oxide release and post-exercise blood pressure reduction in individuals with type-2 diabetes

Influence of tourniquet application on venous blood sampling for serum chemistry, hematological parameters, leukocyte activation and erythrocyte mechanical properties

Quantitative and Comparative Contents of Nitrate and Nitrite in Beta vulgaris L. by Reversed-Phase High-Performance Liquid Chromatography-Fluorescence

Contact Info

Product

Resources

About