Analysis of new aphid lethal paralysis virus (ALPV) isolates suggests evolution of two ALPV species

Liu, Sijun; Vijayendran, Diveena; Carrillo-Tripp, Jimena; Miller, W. Allen; Bonning, Bryony C.

doi:10.1099/vir.0.069765-0

Cited by 27 publications

(34 citation statements)

References 24 publications

(32 reference statements)

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“…Sequence data suggested that three viruses, ALPV [20], RhPV, and ApGIV2, were present in the laboratory colony of A. glycines . In order to isolate A. glycines infected only by ApGlV2, we established single-family aphid colonies.…”

Section: Methodsmentioning

confidence: 99%

Aphis Glycines Virus 2, a Novel Insect Virus with a Unique Genome Structure

Liu

Vijayendran

Chen

et al. 2016

Viruses

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The invasive soybean aphid, Aphis glycines, is a major pest in soybeans, resulting in substantial economic loss. We analyzed the A. glycines transcriptome to identify sequences derived from viruses of A. glycines. We identified sequences derived from a novel virus named Aphis glycines virus 2 (ApGlV2). The assembled virus genome sequence was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and Sanger sequencing, conserved domains were characterized, and distribution, and transmission examined. This virus has a positive sense, single-stranded RNA genome of ~4850 nt that encodes three proteins. The RNA-dependent RNA polymerase (RdRp) of ApGlV2 is a permuted RdRp similar to those of some tetraviruses, while the capsid protein is structurally similar to the capsid proteins of plant sobemoviruses. ApGlV2 also encodes a larger minor capsid protein, which is translated by a readthrough mechanism. ApGlV2 appears to be widespread in A. glycines populations and to persistently infect aphids with a 100% vertical transmission rate. ApGlV2 is susceptible to the antiviral RNA interference (RNAi) pathway. This virus, with its unique genome structure with both plant- and insect-virus characteristics, is of particular interest from an evolutionary standpoint.

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Section: Methodsmentioning

confidence: 99%

Aphis Glycines Virus 2, a Novel Insect Virus with a Unique Genome Structure

Liu

Vijayendran

Chen

et al. 2016

Viruses

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“…Sequence comparison of ALPV isolates (Table 2) suggests two separate species of ALPV. ALPV-AP and ALPV-AM group separately from other ALPV isolates [5].…”

Section: Dicistroviridaementioning

confidence: 99%

“…Aphid lethal paralysis virus (ALPV, Genus: Cripavirus) was first isolated from the bird cherry-oat aphid, Rhopalosiphum padi [21]. Recently, ALPV isolates associated with the honey bee [11,12] and western corn rootworm [5] were discovered. In addition, ALPV sequences were identified in RNA from bat fecal samples [8], although these sequences were most probably derived from viruses infecting insects that were ingested by the bat.…”

Section: Dicistroviridaementioning

confidence: 99%

“…NGS has been used to investigate arthropod viral sequence populations from insects and from insect-derived cell lines [6], as well as from insectivorous bat species [7,8]. As an additional benefit, assembly of RNA virus genomes from NGS data can result in more complete sequence for 5 0 -untranslated regions (UTR) than the use of 5 0 -RACE, which can be blocked by RNA secondary structure in the 5 0 UTR [5,9] (see also Carrillo-Tripp et al, this volume). In this review, we describe recently discovered insect RNA viruses and discuss limitations associated with the use of NGS for virus detection.…”

Section: Introductionmentioning

confidence: 99%

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RNA virus discovery in insects

Liu

Chen

Bonning

2015

Current Opinion in Insect Science

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“…However, several tentative viruses have been identified that show sequence similarity to the members of the genus Iflavirus yet are classified as unassigned viruses at this time (12,16,21,70,71). Previous phylogenetic analysis suggested that iflaviruses have evolved from different origins since the viruses infecting insects from the same order do not form a single clade (3,17).…”

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confidence: 99%

Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

Nouri

Salem

Nigg

et al. 2016

J Virol

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The Asian citrus psyllid, Diaphorina citri, is the natural vector of the causal agent of Huanglongbing (HLB), or citrus greening disease. Together; HLB and D. citri represent a major threat to world citrus production. As there is no cure for HLB, insect vector management is considered one strategy to help control the disease, and D. citri viruses might be useful. In this study, we used a metagenomic approach to analyze viral sequences associated with the global population of D. citri. By sequencing small RNAs and the transcriptome coupled with bioinformatics analysis, we showed that the virus-like sequences of D. citri are diverse. We identified novel viral sequences belonging to the picornavirus superfamily, the Reoviridae, Parvoviridae, and Bunyaviridae families, and an unclassified positive-sense single-stranded RNA virus. Moreover, a Wolbachia prophage-related sequence was identified. This is the first comprehensive survey to assess the viral community from worldwide populations of an agricultural insect pest. Our results provide valuable information on new putative viruses, some of which may have the potential to be used as biocontrol agents. IMPORTANCEInsects have the most species of all animals, and are hosts to, and vectors of, a great variety of known and unknown viruses. Some of these most likely have the potential to be important fundamental and/or practical resources. In this study, we used highthroughput next-generation sequencing (NGS) technology and bioinformatics analysis to identify putative viruses associated with Diaphorina citri, the Asian citrus psyllid. D. citri is the vector of the bacterium causing Huanglongbing (HLB), currently the most serious threat to citrus worldwide. Here, we report several novel viral sequences associated with D. citri. Viruses are the most abundant microbes on our planet (1) and are found in all types of organisms. Insects are the largest and most diverse taxonomic class among animals, representing perhaps half of known animals, with more than one million species recognized worldwide (2). Insects are known to be hosts to viruses belonging to various viral taxa, including the Baculoviridae, Parvoviridae, Flaviviridae, Ascoviridae, Togaviridae, Bunyaviridae, and Rhabdoviridae (3). However, the number of currently described viral species infecting insects is relatively low compared to the number of viruses that have been discovered among prokaryotes, plants, and vertebrates. Furthermore, most of the insect viruses described to date have been discovered because of their pathogenic effects on their insect hosts or because they are pathogens of humans, other vertebrates, or economically important plants. Traditional viral detection methods that require prior knowledge of genome sequences may not be suitable for the discovery of new viruses and, in particular, viruses with a high level of genetic diversity. However, in the past decade, the development of high-throughput next-generation sequencing (NGS) technologies and bioinformatics applications has provided ne...

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Analysis of new aphid lethal paralysis virus (ALPV) isolates suggests evolution of two ALPV species

Cited by 27 publications

References 24 publications

Aphis Glycines Virus 2, a Novel Insect Virus with a Unique Genome Structure

Aphis Glycines Virus 2, a Novel Insect Virus with a Unique Genome Structure

RNA virus discovery in insects

Diverse Array of New Viral Sequences Identified in Worldwide Populations of the Asian Citrus Psyllid (Diaphorina citri) Using Viral Metagenomics

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