Analysis of mutational pattern in multidrug resistant tuberculosis (MDR TB) in a geographically isolated northeastern region of India

Singh, Leimapokpam Shivadutta; Mazumder, Pranab Behari; Sharma, Gauri Dutt

doi:10.9790/3008-09130410

Cited by 3 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mutation in the InhA gene was found to be 41.7% which was similar to the findings of the previous studies [23]. In contrast to our findings, other studies have reported low prevalence (5.4-21.1%) [23,[27][28][29][30][31]. In isoniazid hetero-resistant strains mutation S315T1 and S315T2 in the katG gene were equally prevalent however, variations have been reported in studies [23,27,31,32].…”

Section: Plos Neglected Tropical Diseasessupporting

confidence: 88%

See 1 more Smart Citation

Rapid detection of drug-resistant Mycobacterium tuberculosis by Modified MODS assay suitable for resource-poor settings

Fomda,

Bashir,

Baqal

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Background Cross contamination and biosafety are concerns with the microscopic observation drug susceptibility assay. To address these issues, we modified the MODS technique in the current study. Methodology/Principal findings Two hundred and seventy-five samples were processed on LJ media and drug susceptibility was performed by the Indirect agar proportion method. A modified MODS test was done in tissue culture bottles. GenoType MTBDRplus assay was performed to detect the resistance and mutational pattern associated with the resistances. Sensitivity, specificity, positive predictive value, and negative predictive value for the detection of tuberculosis by modified MODS were 97.44%, 80.00%, 97.44%, and 80.00% respectively. The perfect agreement was seen between modified MODS and the Indirect agar proportion method for drug susceptibility testing of isoniazid (kappa = 0.923) and rifampicin (kappa = 1). The contamination rate, cost and TAT for modified MODS were less as compared to the solid media. In the case of MDR-TB isolates S531L (66.66%) was the most prevalent mutation in the rpoB gene followed by S315T2 mutation (58.33%) and T8C (41.66%) in katG and inhA gene respectively. In hetero-resistant strains, C-15T mutation (37.50%) was the most common followed by A-16G (12.50%) in the inhA gene. In INH mono-resistant strains only two mutations were observed i.e., S-315T1(50%) and C-15T (50%) in the katG and inhA genes respectively. Conclusions/Significance Modified MODS proved to be cost-effective and user-friendly, with minimal risk to the handler and no cross-contamination between samples were observed. Hence, it can be used in low-income countries for early detection of tuberculosis and its resistance.

show abstract

Section: Plos Neglected Tropical Diseasessupporting

confidence: 88%

“…In line with our finding, this mutation (Ser531 ! Leu) was detected among rifampicin-resistant strains varying from 47% to 70.5% in various studies [23,[27][28][29][30]. The katG gene mutations account for the commonest mechanism of resistance for isoniazid.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Rapid detection of drug-resistant Mycobacterium tuberculosis by Modified MODS assay suitable for resource-poor settings

Fomda,

Bashir,

Baqal

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

show abstract

“…Nearly 100,000 cases of serious multi-drug-resistant TB (MDR-TB) are estimated to occur in the country annually, and each MDR TB case costs more than 1 lakh to diagnose and treat. (Singh et al,2014) The incidence:prevalence ratio in India is about 1:32. In case an efficient tuberculosis programme, targeting a sufficient number of sputum-positive prevalence cases in the community, is run for a sufficiently long period of time, it could bring down the prevalence, till probably the point when incidence and prevalence become equal in the community (NACO India, 2012).…”

Section: Introductionmentioning

confidence: 99%

http://www.iosrphr.org/pages/volume4-issue2(part-2)%20-.html

Singh¹

2014

iosrphr

View full text Add to dashboard Cite

About one third of the world's population are infected with tuberculosis and has become a serious global concern of public health. The major problem in controlling tuberculosis is the rapidity and efficacy of detection methods adopted. This study was conducted with the objective to compare the drug susceptibility pattern of Mycobacterium tuberculosis assayed by MODS (Microscopic-observation drugsusceptibility) with that of PM (proportion method) from clinical isolates of North East India. A total of 150 smear positive sputum specimens clinical isolates of M. tuberculosis referred to a clinical lab wa selected. Both the proportion method and MODS were conducted for the collected isolates. DST of MODS were compared with that of PM considering PM as gold standard. MODS detected INH, RIH, STR and EMB resistant isolates at 31% (n = 46/150), 29% (n = 49/150), 19.3& (n = 29/150) and 27.3% (n = 41/150), respectively .Specificity was very high for all the drugs resistance with more than 99% specificity. The accuracy, PPV and NPV of MODS in detection of the four drug resistant isolates was relatively high. DST assay by MODS is relatively simpler. The appreciable performance characteristic in detecting drug resistance including MDR TB may lead to its wider applications in different labs and general hospital for resource limited regions.

show abstract

“…According to the foreign author's researches, test system GenoType MTBDRplus, v.2 has high sensitivity, specificity, predictive value of positive and negative results allows to get results in the first 2 days of inspection, thereby increasing the efficiency of diagnosis chemo-resistant forms of tuberculosis [3,6,7,9,10]. But these researches regarding method`s sensitivity and specificity are different, there is a small amount of national investigations relating to the application GenoType MTBDRplus, v.2 method in the diagnosis of tuberculosis, this resulted in the necessity of our own research.…”

mentioning

confidence: 99%

The effectiveness of GenoType MTBDRplus using in the diagnosis of tuberculosis in Zaporizhzhia region

et al. 2017

View full text Add to dashboard Cite

The method GenoType MTBDRplus, v.2 allows to study clinical material from positive and negative smears, to examine patients with new and previously treated cases of tuberculosis pulmonary and extrapulmonary localization. But now there is a small amount of researches concerning the use of this method in the diagnosis of tuberculosis.Aim. To evaluate the effectiveness of GenoType MTBDRplus, v.2 method using in clinical material research due to the diagnosis of tuberculosis in the Zaporizhzhia region in comparison with standard methods of investigation. Materials and methods.The analysis of 52 results of studies of clinical material using the GenoType MTBDRplus test system, v.2 from patients who were examined and treated in dispensaries of the Zaporizhzhia region in 2016 was carried out. There were 67.3 % of men and 32.7 % of women among the patients. The mean age of the patients was 46.0 ± 1.9 years. The study of the clinical material using the GenoType MTBDRplus test, v.2 was performed according to the standard instructions. Results.The results were positive in 12.5 % of the cases when tested by GenoType MTBDRplus, v.2 (p ˂ 0.05) among negative results of the microscopy of the clinical material. The proportion of coincidence between the cultural and molecular genetic methods was 90 % for sputum and 100 % for other clinical material. In the presence of multiresistance, both methods of investigation coincided in the number of detected cases (50 % for the GenoType MTBDRplus method, v.2 and 42.9 % for the cultural method, respectively, p ˂ 0.05). In comparison with the results of the cultural method, the GenoType MTBDRplus test, v.2 made it possible to establish resistance to rifampicin in 35.7 % (p < 0.05) of patients whose resistance was not determined. False-positive and false-negative results are due, perhaps, to the heterogeneity of strains of mycobacterium tuberculosis in clinical specimens, the absence of viable mycobacteria, the absence of phenotypic manifestations of mycobacterium tuberculosis genetic mutations, and errors in the investigations. The sensitivity of the GenoType MTBDRplus test, v.2 is about 78 %, but the specificity reaches 97 %. At the same time, the sensitivity of the test for multidrug resistance determining reaches 100 %, the specificity is about 89 %. The greater positive predictive value for cases in which the DNA of mycobacteria is determined and the negative predictive value is for cases in which multidrug resistance is determined.Conclusions. This indicates that the using of the GenoType MTBDRplus test, v.2, together with the cultural method of investigation, has a high diagnostic value, high sensitivity (100 %) and specificity (89 %) for the definition of multiresistanc, and makes it possible to obtain a result in a short time compared to culture. Мета роботи -оцінити ефективність застосування методу дослідження клінічного матеріалу з використанням методу GenoType MTBDRplus, v.2 у діагностиці туберкульозу в Запорізькій області порівняно зі стандартними методами до-слідження. Ефективн...

show abstract

Analysis of mutational pattern in multidrug resistant tuberculosis (MDR TB) in a geographically isolated northeastern region of India

Cited by 3 publications

References 18 publications

Rapid detection of drug-resistant Mycobacterium tuberculosis by Modified MODS assay suitable for resource-poor settings

Rapid detection of drug-resistant Mycobacterium tuberculosis by Modified MODS assay suitable for resource-poor settings

http://www.iosrphr.org/pages/volume4-issue2(part-2)%20-.html

The effectiveness of GenoType MTBDRplus using in the diagnosis of tuberculosis in Zaporizhzhia region

Contact Info

Product

Resources

About