Analysis of Multiple Viral Genome Fragments in Adenovirus 7-transformed Hamster Cells

Fujinaga, Kei; Sekikawa, Kenji; Yamazaki, Hiroko; Green, M.

doi:10.1101/sqb.1974.039.01.076

Cited by 12 publications

(2 citation statements)

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“…against 1/Cot, where Cot is the product of the molar concentration of total labeled probe and the reaction time in seconds. These relationships were analyzed according to equations 1 and 2 derived previously, assuming that y copies of a fraction (x/f) and none of the other fraction (1-x/f) ofa probe sequence are present in transformed cells (6,7). The number of viral gene copies, y, can be expressed and calculated as follows: R(kCot)2 + kCot (nmR + nm + R -1)-1 (1) nkCot (1 -RkCot) where n is the molar ratio of unlabeled cellular DNA to labeled viral DNA, k is the reassociation rate constant, R = C../Cd., and m = yx/f.…”

Section: Methodsmentioning

confidence: 99%

Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12

Sawada¹,

Ojima²,

Shimojo³

et al. 1979

J Virol

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Rat cell lines transformed by viral DNA fragments, EcoRI-C and HindIII-G, of adenovirus type 12 DNA were analyzed for the viral transforming DNA sequences present in cell DNAs. Cell lines transformed by the EcoRI-C fragment of adenovirus type 12 DNA (leftmost 16.5% of the viral genome) contain most of the HindIII-G sequences (leftmost 6.8% of the viral genome; the smallest DNA fragment with complete transforming ability) at two to five copies per haploid cell DNA. Cell lines transformed by the HindIII-G fragment contain nearly all of the DNA sequences of the HindIII-G fragment, but at a different frequency depending on the portions of the fragment. The sequence of the AccI-H fragment of adenovirus type 12 DNA (the left part of the HindIII-G; leftmost 4.5% of the viral genome) was detected dominantly in cells transformed by the HindIII-G fragment. Southern blot analysis showed that viral DNA sequences are present at multiple integration sites in high-molecular-weight cell DNA from cells transformed by the EcoRI-C or HindIII-G fragment of adenovirus type 12 DNA. These results suggest that most of the HindIII-G sequences in cells transformed by the HindIII-G fragment are present as fragmented forms.

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Section: Methodsmentioning

confidence: 99%

Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12

Sawada¹,

Ojima²,

Shimojo³

et al. 1979

J Virol

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show abstract

“…Green (6) confirmed this, using highly purified preparations of several strains of these serotypes, and established that Adl4, 16, and 21 were tumorigenic. Cells transformed by members of the three oncogenic human Ad groups contain viral DNA sequences (7)(8)(9)(10)(11) in an integrated form (12) and synthesize viral mRNA (7,13,14 (12,(15)(16)(17). Viral DNA was isolated and purified by the method of Green and Pifia (15,16).…”

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confidence: 99%