Analysis of Monomer Release from Different Composite Resins after Bleaching by HPLC

Abstract

(1) Background: This study aimed to examine the effect of bleaching agents on the release of triethylenae glycol dimethacrylate, 2-hydroxyethyl methacrylate, bisphenol A, urethane dimethacrylate, and bisphenol A-glycidyl methacrylate monomers, which are released from different composite resins, using the high-performance liquid chromatography (HPLC) method. (2) Methods: Ninety disc-shaped specimens were produced and immersed in artificial saliva. After different bleaching applications [office type bleaching (OB) and home type bleaching (HB)], the specimens were immersed in a 75 wt% ethanol/water solution, and the released monomers were analyzed by HPLC at predefined time intervals: 1, 7, and 28 days. The Kruskal–Wallis and Mann–Whitney U tests were conducted for statistical analysis (p = 0.05). (3) Results: The monomers were released at all times from all composite specimens. The monomer release was increased over time. The highest monomer release was detected on day 28. Bleaching applications affected monomer release. No statistical difference was found between OB and HB applications (p > 0.05). The most released monomer was Bisphenol-A in all composites. (4) Conclusion: Given that a residual monomer release from composite resins has a toxic effect and that bleaching treatments increase this release, a treatment protocol should be made in accordance with the manufacturer’s instructions.

Detection of Leachable Components from Conventional and Dental Bulk-Fill Resin Composites (High and Low Viscosity) Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method

“…All measurements were performed once for each sample. The measurements were performed after 1, 7, and 28 days, respectively [ 20 ].…”

Bisphenol A analysis and quantification inconsistencies via HPLC-UV: a systematic review with technical notes

“…Moreover, approximately a quarter of studies (23.64%) utilized HPLC-UV as a technique for quantification of BPA in environmental samples [35][36][37][38][39][40][41][42][43][44][45], mostly from aquatic habitats. 12.73% of the analyzed papers studied the leaching of BPA from consumer products, mostly dentistry resins [31,[46][47][48][49][50][51][52], but also from plastic packaging and bottles [53,54]. The migration of BPA from food packaging could lead to food contamination, but only a few studies (3.64%) directly investigated this phenomenon [55,56].…”

“…Contamination of organisms with BPA was assessed by analyzing the saliva [31,47,77,78], blood [47,57], urine [63], and breast milk [59]. The literature search yielded evidence suggesting that certain dental and orthodontic products may contribute to BPA contamination [31,46,[49][50][51][52][77][78][79].…”

Effect of Different Bleaching Methods on Monomer Release from Aged Microhybrid and Nanohybrid Resin Composites

“…These unreacted monomers can be released from composite restorations [17][18][19][20] and cause irritation and allergic reactions in soft tissue as well as inducing bacterial growth [21,22]. Toxic effects of TEGDMA, UDMA, and BisGMA including cytotoxic, genotoxic, and mutagenic effects have been previously investigated [23]. Issa et al [24] reported that released monomers decreased mitochondrial activity, and Lefeuvre et al [25] found that TEGDMA monomer causes mitochondrial damage.…”