Analysis of microRNA functions

Remsburg, Carolyn; Konrad, Kalin D; Sampilo, Nina Faye; Song, Jia L.

doi:10.1016/bs.mcb.2018.10.005

Cited by 24 publications

(23 citation statements)

References 36 publications

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“…(2) Partial complementarity between lncRNAs and miRNAs. (3) lncRNAs can have multiple MREs for different miRNAs. (4) The overall influence of the sponging effect depends on the specific spatial-temporal distribution.…”

Section: Figurementioning

confidence: 99%

“…Since then, a number of evolutionarily conserved miRNAs have been identified, from plants and fungi to humans, and have been shown to play various roles in biological and pathophysiological processes. To date, thousands of studies on miRNAs using well-developed methods which are now performed routinely (3). Mature miRNAs are short, single-stranded RNA molecules,~22 nucleotides in length, processed from well-characterized precursors through a highly accurate pathway involving a fold-back hairpin structure (4).…”

Section: Introductionmentioning

confidence: 99%

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Research progress on the interactions between long non‑coding RNAs and microRNAs in human cancer (Review)

Binyu

Liu

et al. 2019

Oncol Lett

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Numerous types of molecular mechanisms mediate the development of cancer. Non-coding RNAs (ncRNAs) are being increasingly recognized to play important role in mediating the development of diseases, including cancer. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) are the two most widely studied ncRNAs. Thus far, lncRNAs are known to have biological roles through a variety of mechanisms, including genetic imprinting, chromatin remodeling, cell cycle control, splicing regulation, mRNA decay and translational regulation, and miRNAs regulate gene expression through the degradation of mRNAs and lncRNAs. Although ncRNAs account for a major proportion of the total RNA, the mechanisms underlying the physiological or pathological processes mediated by various types of ncRNAs, and the specific interaction mechanisms between miRNAs and lncRNAs in various physiological and pathological processes, remain largely unknown. Thus, further research in this field is required. In general, the interaction mechanisms between miRNAs and lncRNAs in human cancer have become important research topics, and the study thereof has led to the recent development of related technologies. By providing examples and descriptions, and performing chart analysis, the present study aimed to review the interaction mechanisms and research approaches for these two types of ncRNAs, as well as their roles in the occurrence and development of cancer. These details have far-reaching significance for the utilization of these molecules in the diagnosis and treatment of cancer. Contents 1. Introduction 2. Interactions between lncRNAs and miRNAs 3. Methods of research in to lncRNAs and miRNAs 4. lncRNAs and miRNAs in cancer 5. Conclusion

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Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research progress on the interactions between long non‑coding RNAs and microRNAs in human cancer (Review)

Binyu

Liu

et al. 2019

Oncol Lett

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“…In addition to the antimiRs, there is also the opportunity to mimic or re-express miRNAs by using synthetic RNA duplexes designed to mimic the endogenous functions of the miRNA of interest [107,111].…”

Section: Regulation Of Mirna Expression and Therapeutic Perspectivesmentioning

confidence: 99%

Renal Ca2+ and Water Handling in Response to Calcium Sensing Receptor Signaling: Physiopathological Aspects and Role of CaSR-Regulated microRNAs

Ranieri

2019

IJMS

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Calcium (Ca2+) is a universal and vital intracellular messenger involved in a diverse range of cellular and biological processes. Changes in the concentration of extracellular Ca2+ can disrupt the normal cellular activities and the physiological function of these systems. The calcium sensing receptor (CaSR) is a unique G protein-coupled receptor (GPCR) activated by extracellular Ca2+ and by other physiological cations, aminoacids, and polyamines. CaSR is the main controller of the extracellular Ca2+ homeostatic system by regulating parathyroid hormone (PTH) secretion and, in turn, Ca2+ absorption and resorption. Recent advances highlight novel signaling pathways activated by CaSR signaling involving the regulation of microRNAs (miRNAs). miRNAs are naturally-occurring small non-coding RNAs that regulate post-transcriptional gene expression and are involved in several diseases. We previously described that high luminal Ca2+ in the renal collecting duct attenuates short-term vasopressin-induced aquaporin-2 (AQP2) trafficking through CaSR activation. Moreover, we demonstrated that CaSR signaling reduces AQP2 abundance via AQP2-targeting miRNA-137. This review summarizes the recent data related to CaSR-regulated miRNAs signaling pathways in the kidney.

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“…MicroRNAs (miRNAs/miRs) are a class of small endogenous non-coding RNA molecules with 15-25 nucleotides in length (9,10). The first miRNA has been discovered in nematodes (11).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN

Wang

et al. 2020

Exp Ther Med

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Osteosarcoma (OS) is a malignant bone tumor with a poor prognosis. Accumulated evidence has suggested that microRNAs (miRNAs/miRs) may function as either oncogenes or tumor suppressors, which are associated with tumorigenesis and the progression of different types of cancer. In the present study, the role of miR-208a-3p in OS was investigated. The expression levels of miR-208a-3p in OS tissues and cell lines were determined via reverse transcription-quantitative PCR (RT-qPCR). MTT and colony formation assays were performed to verify the proliferation rate of OS cells. In addition, the effects of miR-208a-3p on the migration and invasion of OS cells were revealed using wound-healing and Transwell assays, respectively. Furthermore, the association between miR-208a-3p and phosphatase and tensin homolog (PTEN) 3'-untranslated region was determined via luciferase reporter assays, western blot and RT-qPCR analysis. The results indicated that miR-208a-3p was upregulated in OS tissues and cell lines compared with adjacent normal tissues and human osteoblastic cells, respectively. miR-208a-3p overexpression promoted and miR-208a-3p knockdown inhibited OS cells proliferation and metastatic potential. Additionally, PTEN was validated as a direct target of miR-208a-3p and its expression was negatively associate with that of miR-208a-3p in OS cells. Taken together, these results may suggest that miR-208a-3p promoted OS cells proliferation and metastatic potential via targeting PTEN. Therefore, miR-208a-3p may be considered as a diagnostic biomarker for OS.

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Analysis of microRNA functions

Cited by 24 publications

References 36 publications

Research progress on the interactions between long non‑coding RNAs and microRNAs in human cancer (Review)

Research progress on the interactions between long non‑coding RNAs and microRNAs in human cancer (Review)

Renal Ca2+ and Water Handling in Response to Calcium Sensing Receptor Signaling: Physiopathological Aspects and Role of CaSR-Regulated microRNAs

MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN

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