Analysis of Metallothionein Expression in Human Cancers.

Bay, Boon‐Huat; Jin, Rong; Jayasurya, Anita

doi:10.1267/ahc.34.171

Cited by 11 publications

(5 citation statements)

References 51 publications

(48 reference statements)

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“…The expression patterns of MT isoforms have been reported in various human tumors, such as the increase in mRNA levels of MT1A, MT1E, MT1F, MT1G, MT1H, and MT1X (but not MT1B), in breast cancer tissues (Bay et al, 2001). MT2A mRNA transcript has been reported to positively correlate with cell proliferation and histological grading of breast cancer (Jin et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of Intrahepatic Cholangiocarcinoma

Subrungruanga¹,

Thawornkunob²,

Chawalitchewinkoon-Petmitrc³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Intrahepatic cholangiocarcinoma (ICC) is ranked as one of the top five causes of cancer-related deaths. ICC in Thai patients is associated with infection with the liver fluke, Opisthorchis viverrini, but the molecular basis for development remains unclear. The present study employed a microarray approach to compare gene expression profiles of ICCs and normal liver tissues from the same patients residing in Northeast Thailand, a region with a high prevalence of liver fluke infection. In ICC samples, 2,821 and 1,361 genes were found to be significantly up-and down-regulated respectively (unpaired t-test, p<0.05; fold-change ≥2.0). For validation of the microarray results, 7 up-regulated genes (FXYD3, GPRC5A, CEACAM5, MUC13, EPCAM, TMC5, and EHF) and 3 downregulated genes (CPS1, TAT, and ITIH1) were selected for confirmation using quantitative RT-PCR, resulting in 100% agreement. The metallothionine heavy metal pathway contains the highest percentage of genes with statistically significant changes in expression. This study provides exon-level expression profiles in ICC that should be fruitful in identifying novel genetic markers for classifying and possibly early diagnosis of this highly fatal type of cholangiocarcinoma.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Profiling of Intrahepatic Cholangiocarcinoma

Subrungruanga¹,

Thawornkunob²,

Chawalitchewinkoon-Petmitrc³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Nevertheless, these case-control and cohort studies give us inconsistent results regarding the association of MTs and tumor histology, staging, grading, prognosis, and survival. Although there is a number of good systematic reviews [2] , [3] , [11] , [13] , [14] , [26] – [28] , [29,] with particular interest in breast tumors [30] , [31] , a meta-analytic approach has not been employed yet. Thus, the aim of this study is to evaluate the associations between immunohistochemical MT staining and clinicopathological conditions, tumor type, stage, grade, prognosis, and survival using the meta-analysis.…”

Section: Introductionmentioning

confidence: 99%

Metallothionein – Immunohistochemical Cancer Biomarker: A Meta-Analysis

et al. 2014

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Metallothionein (MT) has been extensively investigated as a molecular marker of various types of cancer. In spite of the fact that numerous reviews have been published in this field, no meta-analytical approach has been performed. Therefore, results of to-date immunohistochemistry-based studies were summarized using meta-analysis in this review.Web of science, PubMed, Embase and CENTRAL databases were searched (up to April 30, 2013) and the eligibility of individual studies and heterogeneity among the studies was assessed. Random and fixed effects model meta-analysis was employed depending on the heterogeneity, and publication bias was evaluated using funnel plots and Egger's tests.A total of 77 studies were included with 8,015 tissue samples (4,631 cases and 3,384 controls). A significantly positive association between MT staining and tumors (vs. healthy tissues) was observed in head and neck (odds ratio, OR 9.95; 95% CI 5.82–17.03) and ovarian tumors (OR 7.83; 1.09–56.29), and a negative association was ascertained in liver tumors (OR 0.10; 0.03–0.30). No significant associations were identified in breast, colorectal, prostate, thyroid, stomach, bladder, kidney, gallbladder, and uterine cancers and in melanoma. While no associations were identified between MT and tumor staging, a positive association was identified with the tumor grade (OR 1.58; 1.08–2.30). In particular, strong associations were observed in breast, ovarian, uterine and prostate cancers. Borderline significant association of metastatic status and MT staining were determined (OR 1.59; 1.03–2.46), particularly in esophageal cancer. Additionally, a significant association between the patient prognosis and MT staining was also demonstrated (hazard ratio 2.04; 1.47–2.81). However, a high degree of inconsistence was observed in several tumor types, including colorectal, kidney and prostate cancer.Despite the ambiguity in some tumor types, conclusive results are provided in the tumors of head and neck, ovary and liver and in relation to the tumor grade and patient survival.

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“…Three-dimensional structure of rat liver metallothionein. The molecular modelwas generated using RasMol software as previously described by Bay at al (49)…”

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confidence: 99%

Metallothionein as a Prognostic Biomarker in Breast Cancer

Bay

Jin

Huang

et al. 2006

Exp Biol Med (Maywood)

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Breast cancer is the most common cancer in women, with a general upward trend in incidence. Basic and clinical breast cancer research has continued at a rapid pace, in the endeavor to understand the biology of the disease so as to improve management of patients. Besides traditional pathological indicators, expression of molecular markers in breast cancer has also been comprehensively investigated. This paper will focus on the prognostic utility of metallothioneins (MTs), a family of low molecular weight metal binding proteins encoded by at least 10 functional MT genes that are associated with cell proliferation in breast cancer. Evidence that MT is a potential prognostic biomarker for breast cancer is supported by many reports in the literature. Expression of the MT protein has been detected by immunohistochemistry in a significant portion of invasive ductal breast cancers. MT expression has also been well studied in association with traditional clinico-pathological parameters of breast cancers. Generally, higher MT expression in breast cancers is predictive of worse patient outcomes. The relationship of MT isoforms to histological grade, estrogen receptor (ER) status, and prognosis will also be discussed.

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Analysis of Metallothionein Expression in Human Cancers.

Cited by 11 publications

References 51 publications

Gene Expression Profiling of Intrahepatic Cholangiocarcinoma

Gene Expression Profiling of Intrahepatic Cholangiocarcinoma

Metallothionein – Immunohistochemical Cancer Biomarker: A Meta-Analysis

Metallothionein as a Prognostic Biomarker in Breast Cancer

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