Analysis of mandipropamid residual levels through systematic method optimization against the matrix complexity of sesame leaves using HPLC/UVD

Abstract

An analytical method was developed to detect mandipropamid residues in sesame leaves using high-performance liquid chromatography-ultraviolet detection. Samples were extracted with acetonitrile and were prepurified using a solid-phase extraction (SPE) cartridge with an additional dispersive-SPE (d-SPE) sorbent application. The method was validated using an external calibration curve prepared using pure solvent. The linearity was excellent with determination coefficient = 1. The limits of detection and quantification were 0.003 and 0.01 mg/kg, respectively. Recoveries at three spiking levels - 0.1, 0.5, and 1.0 mg/kg - were in the range 80.3-90.7% with relative standard deviations <2%. This method was applied to field-treated samples collected from two different areas, Gwangju and Muan, in the Republic of Korea and the half-lives were similar, 5.10 and 5.41 days, respectively. The pre-harvest residue limit was also predicted for both sites. The proposed method is sensitive and able to quantify trace amounts of mandipropamid in leafy vegetables. The combination of SPE and d-SPE effectively removed the matrix components in sesame leaves. Copyright © 2015 John Wiley & Sons, Ltd.

Analytical approach, dissipation pattern and risk assessment of pesticide residue in green leafy vegetables: A comprehensive review

“…The complexity in sample treatment corresponds with the existing matrix interferences and the use of separation techniques, most commonly gas chromatography (Al Mahmud et al, ; D'Antuono et al, ; Farina et al, ; González‐Rodríguez, Rial‐Otero, Cancho‐Grande, & Simal‐Gándara, ; Ikeura, Kobayashi, & Tamaki, ; Park et al, ; Rahman, Sharma, et al, ; Srivastava, Trivedi, Srivastava, Lohani, & Srivastava, ; Tanaka et al, ; Van Dyk, Bouwman, Barnhoorn, & Bornman, ; Walorczyk, ) or liquid chromatography (Farha, Rahman, Abd El‐Aty, Jung, et al, ; Farha, Rahman, Abd El‐Aty, Kim, et al, ; S. W. Kim, Abd El‐Aty, et al, ; S. W. Kim, Rahman, et al, ; Lehotay & Cook, ; Liu et al, ; Pan, Xia, & Liang, ; Park et al, , ; Rahman et al, ; Wołejko et al, ).…”

“…All these factors led to complex matrices. To overcome the inference problems, liquid chromatography with ultraviolet absorbance detection (LC/UVD) is best (Farha, Rahman, Abd El‐Aty, Jung, et al, ; Farha, Rahman, Abd El‐Aty, Kim, et al, ; Liu et al, ), and to tackle suppression problems, the appropriate method might be LC associated with tandem mass spectrometry (LC–MS/MS) (S. W. Kim, Abd El‐Aty, et al, ; S. W. Kim, Rahman, et al, ; Na et al, ; Pan et al, ; Park et al, ; Rahman et al, ; Yang et al, ). Suppression problems increase the limits of quantification (LOQ) to equal to the MRL, which is expected to be 1/10.…”

Two chromatographic methods for the quantitative determination of some pesticides applied for cucumber pests in Egypt

“…Results obtained by the proposed chromatographic methods for the determination of pure samples of MAN, MET, THO and CAR were statistically compared to those obtained by the reported HPLC methods . The results showed no significant differences between them (Table ).…”

“…HPLC methods were used for determination of THO and CAR in vegetables . MAN and MET residues in vegetable leaves were determined each one individually by HPLC methods . All the previously mentioned methods cannot determine the selected pesticides as a quaternary mixture.…”

Quick, easy, cheap, effective, rugged, and safe sample preparation approach for pesticide residue analysis using traditional detectors in chromatography: A review

“…Even after using c‐SPE (silica/ Florisil®) to purify 5 g of leafy vegetables (perilla leaves), the target analyte was not protected against interference in the UV detection. Additional d‐SPE cleanup has been employed to overcome this problem, which requires additional time and labor . In this case, the double‐layer c‐SPE concept proposed by Lehotay et al.…”