Analysis of major milk whey proteins by immunoaffinity capillary electrophoresis coupled with MALDI‐MS

Gasilova, Natalia; Gassner, Anne‐Laure

doi:10.1002/elps.201200079

Cited by 39 publications

(32 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Detailed method description for the analysis of β‐lactoglobulin in infant foods by CE‐LIF using on‐capillary derivatization, was recently described by Garrido‐Medina et al . Using immunoaffinity CE, β‐lactoglobulin and α‐lactalbumin were immunocaptured inside the capillary (coated with hydroxypropylcellulose) through the use of magnetic beads functionalized with antibodies . After elution from the beads, analyte focusing and separation were performed by transient ITP.…”

Section: Peptides and Proteinsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

View full text Add to dashboard Cite

In this work, the analysis of foods and food components using capillary electromigration methods is reviewed. The present work presents and discusses the main CE applications performed in Food Science and Technology including the new field of Foodomics, reviewing recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food interactions and food processing. The CE analysis of a large variety of food-related molecules with different chemical properties, including amino acids, peptides, proteins, phenolic compounds, carbohydrates, DNA fragments, vitamins, toxins, pesticides, additives, and other minor compounds is described. The use of microchips, CE-MS, and chiral-CE in food analysis is also discussed as well as other current and foreseen trends in this area of research. Following the previous review by Castro-Puyana et al. (Electrophoresis, 2012, 33, 147–167), the current review covers the papers that were published from February 2011 to February 2013.

show abstract

Section: Peptides and Proteinsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In the last decade, many different magnetic beads (MBs) have become commercially available with a wide range of surface chemistries to easily and reproducibly couple many types of microorganisms, cells or biological molecules, including Ab . The robustness and versatility of the commercial MBs and the simplicity of operation are rapidly expanding the application areas, including IA‐SPE‐CE . Since the pioneering work of Rashkovetsky et al.…”

Section: Introductionmentioning

confidence: 99%

“…Since the pioneering work of Rashkovetsky et al. , several authors have described the use of IA‐MBs in capillary or microchip format with ultraviolet (UV) , fluorescence or MALDI‐MS detection, but not yet with fully on‐line ESI‐MS detection. One of the great advantages of MBs in IA‐SPE‐CE is that facilitate the packing procedures and preparation of fritless microcartridges or microrreactors, because permanent magnets or electromagnets can be used to trap or move the particles.…”

Section: Introductionmentioning

confidence: 99%

Analysis of serum transthyretin by on-line immunoaffinity solid-phase extraction capillary electrophoresis mass spectrometry using magnetic beads

et al. 2016

View full text Add to dashboard Cite

In this paper, an on-line immunoaffinity solid-phase extraction capillary electrophoresis mass spectrometry (IA-SPE-CE-MS) method using magnetic beads (MBs) is described for the analysis of serum transthyretin (TTR), which is a protein related to different types of amyloidosis. First, purification of TTR from serum was investigated by off-line immunoprecipitation and CE-MS. The suitability of three Protein A (ProA) MBs (Protein A Ultrarapid Agarose(TM) (UAPA), Dynabeads(®) Protein A (DyPA) and SiMAG-Protein A (SiPA) and AffiAmino Ultrarapid Agarose(TM) (UAAF) MBs to prepare an IA sorbent with a polyclonal antibody (Ab) against TTR, was studied. In all cases, results were repeatable and it was possible the identification and the quantitation of the relative abundance of the six most abundant TTR proteoforms. Although recoveries were the best with UAPA MBs, UAAF MBs were preferred for on-line immunopurification because Ab was not eluted from the MBs. Under the optimized conditions with standards in IA-SPE-CE-MS, microcartridge lifetime (>20 analyses/day) and repeatability (2.9 and 4.3% RSD for migration times and peak areas) were good, the method was linear between 5 and 25 μg/mL and LOD was around 1 μg/mL (25 times lower than by CE-MS, ≈25 μg/mL). A simple off-line sample pretreatment based on precipitation of the most abundant proteins with 5% (v/v) of phenol was necessary to clean-up serum samples. The potential of the on-line method to screen for familial amyloidotic polyneuropathy type I (FAP-I), which is the most common hereditary systemic amyloidosis, was demonstrated analysing serum samples from healthy controls and FAP-I patients.

show abstract

“…To avoid this negative effect, separation techniques are typically coupled with MS for unambiguous allergen detection [2,[83][84]. In this case, one possibility is to perform the direct MS detection of the allergen molecule itself, as it was realized by ultra-performance LC-MS [91] and IACE-MALDI-MS [92] for the quantification of milk whey allergens. Another approach, which is typically applied for LC-MS/MS, is based on the detection of target characteristic peptides from the allergenic molecule and not the entire allergen (Figure 6) [71].…”

Section: Separation Techniques and Msmentioning

confidence: 99%

Bioanalytical Methods for Food Allergy Diagnosis, Allergen Detection and New Allergen Discovery

Gasilova

2015

Bioanalysis

Self Cite

View full text Add to dashboard Cite

For effective monitoring and prevention of the food allergy, one of the emerging health problems nowadays, existing diagnostic procedures and allergen detection techniques are constantly improved. Meanwhile, new methods are also developed, and more and more putative allergens are discovered. This review describes traditional methods and summarizes recent advances in the fast evolving field of the in vitro food allergy diagnosis, allergen detection in food products and discovery of the new allergenic molecules. A special attention is paid to the new diagnostic methods under laboratory development like various immuno- and aptamer-based assays, including immunoaffinity capillary electrophoresis. The latter technique shows the importance of MS application not only for the allergen detection but also for the allergy diagnosis.

show abstract

Analysis of major milk whey proteins by immunoaffinity capillary electrophoresis coupled with MALDI‐MS

Cited by 39 publications

References 45 publications

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Analysis of serum transthyretin by on-line immunoaffinity solid-phase extraction capillary electrophoresis mass spectrometry using magnetic beads

Bioanalytical Methods for Food Allergy Diagnosis, Allergen Detection and New Allergen Discovery

Contact Info

Product

Resources

About