Analysis of major classes of plant lipids by high-performance liquid chromatography with flame ionization detection

Moreau, Robert A.; Asmann, Paul T.; Norman, H.D.

doi:10.1016/0031-9422(90)85167-e

Cited by 56 publications

(20 citation statements)

References 13 publications

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“…These results are consistent with in vitro CL synthase assays with mung bean mitochondrial inner membranes or with a protein extract containing CL synthase purified by guest, on May 11, 2018 www.jlr.org Downloaded from enzyme (56). The high abundance of highly unsaturated molecular species of CL, and their increase during phosphate deficiency and growth in darkness, point toward a critical role of these unsaturated CL forms in the inner mitochondrial membranes.…”

Section: Peak With the M/z Of [M-h]supporting

confidence: 83%

“…by guest, on May 11, 2018 www.jlr.org Downloaded from 500 mg fresh weight provided the most reproducible results (Fig. 6A).…”

Section: Validation Of CL Quantification From Spinach Leavesmentioning

confidence: 78%

“…CL, a crucial phospholipid in bacterial cells and in mitochondria of eukaryotes, has previously been analyzed in plants by chromatographic methods, including TLC, GC, and HPLC (15,22,24,25). These techniques provided data on the content of plant CL but failed to give the information of molecular species composition.…”

Section: Discussionmentioning

confidence: 99%

“…, were used for quantification in alternative approaches (28,30,31,35 by guest, on May 11, 2018 www.jlr.org Downloaded from DW of total CL (Fig. 8).…”

Section: mentioning

confidence: 99%

“…Combination of TLC and GC or GC/MS provides the means for quantification of individual fatty acids in CL (24). HPLC was used to separate and quantify major lipid classes in plants, and trace amount of CL can be detected in some plant tissues (25). MS/MS with LC separation or direct infusion was developed for profiling plant membrane lipids, providing detailed information on most lipids except CL (26,27).…”

mentioning

confidence: 99%

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Molecular species composition of plant cardiolipin determined by liquid chromatography mass spectrometry

Zhou

Peisker

Dörmann

2016

Journal of Lipid Research

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Section: Peak With the M/z Of [M-h]supporting

confidence: 83%

“…by guest, on May 11, 2018 www.jlr.org Downloaded from 500 mg fresh weight provided the most reproducible results (Fig. 6A).…”

Section: Validation Of CL Quantification From Spinach Leavesmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

“…, were used for quantification in alternative approaches (28,30,31,35 by guest, on May 11, 2018 www.jlr.org Downloaded from DW of total CL (Fig. 8).…”

Section: mentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular species composition of plant cardiolipin determined by liquid chromatography mass spectrometry

Zhou

Peisker

Dörmann

2016

Journal of Lipid Research

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A rapid quantitative method for the analysis of sesquiterpene phytoalexins by high performance liquid chromatography

Moreau

Preisig

Osman

1992

Phytochemical Analysis

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A new high performance liquid chromatographic technique has been developed for the rapid analysis of sesquiterpene phytoalexins such as capsidiol, rishitin, lubimin and phytuberol. This method employs a cyanopropyl‐bonded phase column with an isocractic mixture of hexane and isopropanol. When used with a flame ionization detector the lower limits of detection are about 0.1 μg and the response of the detector is linear in the range 0.1–30 μg. With the UV detector at 205 nm, the lower limits of detection are about 0.1 μg for capsidiol and rishitin and 0.5 μg for lubimin, phytuberol and debneyol. The response of the UV detector is linear in the range 0.5–30 μg. Although both detectors proved to be useful, the signal response with the flame ionization detector was proportional to the mass of each of the phytoalexins, while the signal with the UV detector was proportional to the number of carbon‐carbon double bonds in each of the compounds.

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Separation, identification and quantification of monomers from cutin polymers by high performance liquid chromatography and evaporative light scattering detection

Gérard

Osman

Fett

et al. 1992

Phytochemical Analysis

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A silicic acid high performance liquid chromatographic method which utilizes an evaporative light scattering detector has been developed for the separation of cutin monomers. This binary gradient system was successfully applied to separate underivatized monomers of several fruit cutins including those from apple cvs. Golden Delicious and Red Delicious (Malus pumila), tomato (Lycopersicon esculentum), grapefruit (Citrus paradisi) green pepper (Capsidum annum) and pumpkin (Cucurbita pepo). All cutin monomers, from the least polar, monohydroxy saturated and unsaturated fatty acids, to the most polar, trihydroxy saturated and unsaturated fatty acids, were separated. Monomer identity was confirmed by gas chromatography‐mass spectromety of collected peaks. The gradient was also able to separate positional isomers of dihydroxy fatty acids, a separation not yet achieved by gas chromatography. The relationship between detector response and the mass of five common cutin monomers was obtained.

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Analysis of major classes of plant lipids by high-performance liquid chromatography with flame ionization detection

Cited by 56 publications

References 13 publications

Molecular species composition of plant cardiolipin determined by liquid chromatography mass spectrometry

Molecular species composition of plant cardiolipin determined by liquid chromatography mass spectrometry

A rapid quantitative method for the analysis of sesquiterpene phytoalexins by high performance liquid chromatography

Separation, identification and quantification of monomers from cutin polymers by high performance liquid chromatography and evaporative light scattering detection

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