Analysis of long-term monomer elution from bulk-fill and conventional resin-composites using high performance liquid chromatography

Alshali, Ruwaida Z.; Salim, Nesreen A.; Sung, Rehana; Satterthwaite, Julian D.; Silikas, Nick

doi:10.1016/j.dental.2015.10.006

Cited by 76 publications

(122 citation statements)

References 55 publications

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“…This in vitro elution experiment could demonstrate the presence of these four monomers, together with CQ and the different forms of BisEMA (i.e., BisEMA‐3 and BisEMA‐10). These results are in accordance with previous studies . Venus Pearl is based on TCD‐DI‐HEA and UDMA.…”

Section: Resultssupporting

confidence: 93%

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

et al. 2017

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Resin-based dental materials have raised debates concerning their safety and biocompatibility, resulting in a growing necessity of profound knowledge on the quantity of released compounds into the oral cavity. In this context, the aim of this study was to develop a comprehensive and reliable procedure based on liquid chromatography with mass spectrometry for the simultaneous analysis of various leached compounds (including bisphenol A based compounds) in samples from in vitro experiments. Different experiments were performed to determine the optimal analytical parameters, comprising mass spectrometry parameters, chromatographic separation conditions, and sample preparation. Four internal standards were used as follows: deuterated diethyl phthalate and bisphenol A (commercially available), and deuterated analogues of triethylene glycol dimethacrylate and urethane dimethacrylate (custom-made). The optimized method was validated for linearity of the calibration curves and the associated correlation coefficient, lower limit of quantification, higher limit of quantification, and intra- and interassay accuracy and precision. Additionally, the developed liquid chromatography with tandem mass spectrometry method was applied to the analysis of leaching compounds from four resin-based dental materials. The results indicated that this method is suitable for the analysis of different target compounds leaching from dental materials. This method might serve as a valuable basis for quick and accurate quantification of leached compounds from resin-based dental materials in biological samples.

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Section: Resultssupporting

confidence: 93%

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

et al. 2017

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“…Dental biomaterials should ideally be nontoxic to both hard and soft oral tissues. Nevertheless many in vitro investigations have indicated that the polymerization reaction that generates the cross-linked polymer matrix from the dimethacrylate resin monomer is never complete and cytotoxic reactions occur due to the release of unpolymerized monomers 10,[22][23][24] .…”

Section: Discussionmentioning

confidence: 99%

“…Cytotoxicity mechanisms are predominantly associated with the leaching of these residual monomers formed during monomer-polymer conversion 9) . For bulk-fill composites a conversion rate higher than 55% is in the clinically acceptable range but is still lower than that of conventional composites 10) .…”

Section: Introductionmentioning

confidence: 86%

Cytotoxic effects of contemporary bulk-fill dental composites: A real-time cell analysis

et al. 2020

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The aim of this study was to evaluate the cytotoxicity of contemporary flowable and paste-like bulk-fill dental composites by using a real-time cell analysis. In the present paper, cytotoxicity levels of five flowable, five paste-like bulk-fill composite materials and one conventional flowable, one conventional paste-like resin composite were examined on L929 mouse fibroblast cell line. After seeding 25,000 cells/300 μL/well cell suspensions into the wells of an E-plate, test materials were added and observed at every 30 min intervals for 72 h. Kruskal Wallis H and Mann Whitney U multiple comparison tests were used to analyze the results. Pre-reacted glass-ionomer (PRG) containing bulk-fill composites were severely toxic at all time points (24, 48 and 72 h, p<0.05). None of the tested composites demonstrated high cell viability (>70%) at 48 and 72 h. Flowable and paste-like composites of the same brand exhibited similar cytotoxic properties (p>0.05).

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“…Furthermore, despite increased increment thickness of bulk-fill composites, monomer elution from these materials has been shown to be comparable to that of conventional composites, with the rate of elution being dependent on monomer molecular weight [51]. Beside the degree of conversion [8,52], the cross-link density of the polymer network affects the extent of monomer elution [53].…”

Section: Discussionmentioning

confidence: 99%

Genotoxic potential of dental bulk-fill resin composites

et al. 2017

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Analysis of long-term monomer elution from bulk-fill and conventional resin-composites using high performance liquid chromatography

Cited by 76 publications

References 55 publications

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

Cytotoxic effects of contemporary bulk-fill dental composites: A real-time cell analysis

Genotoxic potential of dental bulk-fill resin composites

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