Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS

Abstract

Resin-based dental materials have raised debates concerning their safety and biocompatibility, resulting in a growing necessity of profound knowledge on the quantity of released compounds into the oral cavity. In this context, the aim of this study was to develop a comprehensive and reliable procedure based on liquid chromatography with mass spectrometry for the simultaneous analysis of various leached compounds (including bisphenol A based compounds) in samples from in vitro experiments. Different experiments were performed to determine the optimal analytical parameters, comprising mass spectrometry parameters, chromatographic separation conditions, and sample preparation. Four internal standards were used as follows: deuterated diethyl phthalate and bisphenol A (commercially available), and deuterated analogues of triethylene glycol dimethacrylate and urethane dimethacrylate (custom-made). The optimized method was validated for linearity of the calibration curves and the associated correlation coefficient, lower limit of quantification, higher limit of quantification, and intra- and interassay accuracy and precision. Additionally, the developed liquid chromatography with tandem mass spectrometry method was applied to the analysis of leaching compounds from four resin-based dental materials. The results indicated that this method is suitable for the analysis of different target compounds leaching from dental materials. This method might serve as a valuable basis for quick and accurate quantification of leached compounds from resin-based dental materials in biological samples.

Bisphenol A in human saliva and urine before and after treatment with dental polymer‐based restorative materials

“…In the present study, we used LC/MS/MS with an isotopically labeled internal standard (D16‐BPA) for the analysis of BPA. This is the method of choice for measuring the content of BPA in biological samples . Quality control samples and field blanks were analyzed together with the samples of saliva and urine.…”

Bisphenol A concentration in human saliva related to dental polymer-based fillings

“…To achieve high sensitivity and high specificity, LC/MS/MS with an isotopically labeled internal standard (D16-BPA) was used. The LC/MS/MS is the method of choice for the determination of BPA in biological samples(139). BPA is a widespread chemical, and it might be found ubiquitously.…”

An Overview of Analytical Methods for Bisphenol A

“…For this purpose, scientists recovered BPA from samples by using various extraction techniques and worked on BPA determination with many different methods. They are various chromatographic methods such as gas chromatography-mass spectrometry GC-MS [34], high performance liquid chromatography-UV/Vis detector (HPLC-UV) [35,36], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [5,37] and ultra high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) [38,39], electrochemical methods such as amperometry [40,41] [47]. However, interference effects that may occur in the analysis of complex samples made with non-mass-based detectors such as UV, ECD and FL may cause erroneous results.…”