Analysis of Linear Macromolecule Transport through Aluminum Anodic Oxide Membranes by Pore Model.

Ichimura, Shigetoshi; Tsuru, Toshinori; Nakao, Shin-ichi; Shoji, K.

doi:10.1252/jcej.33.141

Cited by 7 publications

(17 citation statements)

References 30 publications

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“…The transport of long, linear molecules in solution through small pores has relevance for a number of processes including gel permeation chromatography [1-4], ultra filtration [5, 6], protein structural characterization [7, 8], gene transfer and RNA translocation [9-11] or the excretion and assembly of proteins and other molecules through cellular nanopores [12, 13]. The process of polymer translocation through nanoscale pores is also a powerful tool in the development of methods to separate and perhaps organize molecules.…”

Section: Introductionmentioning

confidence: 99%

“…The use of r H has led to discrepancies between experimentally-observed and theoretically-predicted macromolecular solute diffusivities [5]; and in misleading results from the calculation of the hydrodynamic resistance to solute motion in pores. Theoretically, flexible polymers with radius r H should transport through the pores faster than spheres of radius r H due to decreased predicted hydrodynamic resistances.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, numerous efforts to develop hydrodynamic models that more accurately describe the transport problem have been attempted [57, 58, 61-66]. As it turns out, the radius of gyration ( r F ) may be a better parameter to characterize the size of transporting flexible polymers [5]. r F is the radius of an isolated chain in the presence of excluded volume effects, and for a bulk dilute solution it is scaled as:

r_{F} ≅ a N^{ν}

…”

Section: Introductionmentioning

confidence: 99%

“…One model used to estimate the transport of flexible polymers through nanopore membranes is the “open pore model” [5, 73-76]. Originally developed for rigid spherical solutes, the open pore model has also been shown to accurately predict the transport of linear macromolecules of similar weight to collagen through membranes with highly regulated cylindrical pores of dimensions in the 10 1 nm range [5].…”

Section: Introductionmentioning

confidence: 99%

“…Originally developed for rigid spherical solutes, the open pore model has also been shown to accurately predict the transport of linear macromolecules of similar weight to collagen through membranes with highly regulated cylindrical pores of dimensions in the 10 1 nm range [5]. In the open pore model, r F is reportedly a better characteristic dimension for transport behaviour than r H [5].…”

Section: Introductionmentioning

confidence: 99%

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Optimizing collagen transport through track-etched nanopores

Bueno¹,

Ruberti²

2008

Journal of Membrane Science

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Polymer transport through nanopores is a potentially powerful tool for separation and organization of molecules in biotechnology applications. Our goal is to produce aligned collagen fibrils by mimicking cell-mediated collagen assembly: driving collagen monomers in solution through the aligned nanopores in track-etched membranes followed by fibrillogenesis at the pore exit. We examined type I atelo-collagen monomer transport in neutral, cold solution through polycarbonate track-etched membranes comprising 80-nm-diameter, 6-μm-long pores at 2% areal fraction. Source concentrations of 1.0, 2.8 and 7.0 mg/ml and pressure differentials of 0, 10 and 20 inH 2 O were used. Membrane surfaces were hydrophilized via covalent poly(ethylene-glycol) binding to limit solute-membrane interaction. Collagen transport through the nanopores was a non-intuitive process due to the complex behavior of this associating molecule in semi-dilute solution. Nonetheless, a modified open pore model provided reasonable predictions of transport parameters. Transport rates were concentration-and pressure-dependent, with diffusivities across the membrane in semi-dilute solution two-fold those in dilute solution, possibly via cooperative diffusion or polymer entrainment. The most significant enhancement of collagen transport was accomplished by membrane hydrophilization. The highest concentration transported (5.99±2.58 mg/ml) with the highest monomer flux (2.60±0.49 ×10 3 molecules s -1 pore -1 ) was observed using 2.8 mg collagen/ml, 10 inH 2 O and hydrophilic membranes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

r_{F} ≅ a N^{ν}

…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimizing collagen transport through track-etched nanopores

Bueno¹,

Ruberti²

2008

Journal of Membrane Science

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Diffusion of semi‐flexible polyelectrolyte through nanochannels

Faucher

Zhu

2009

AIChE Journal

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in Wiley InterScience (www.interscience.wiley.com).The diffusion of sodium polystyrene sulfonate through polycarbonate nanochanels was studied in salt-free dilute aqueous solution. A stronger molecular weight dependence of diffusion was observed compared to free diffusion in dilute solution. t . For charged polyelectrolytes, the transition to 1-D diffusion therefore begins before the polymer radius of gyration exceeds the channel size contrary to model assumptions. We attribute this to the charged nature of the polyelectrolytes causing extended chain conformations.

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